Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein
10.1128/jb.159.3.811-819.1984
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The genes for alkaline protease (apr[BamP]) and neutral protease (npr[BamP]) from Bacillus amyloliquefaciens have been isolated and expressed in Bacillus subtilis. The DNA sequences of apr[BamP] and npr[BamP] revealed, in each case, the presence of a large open reading frame. The inferred amino acid sequence of either gene contained a signal sequence and an additional polypeptide sequence ('pro' sequence) preceding the mature protein. Based on DNA sequence, the start point of translation has been identified as amino acid residue - 107 for apr[BamP] and -221 for npr[BamP]. To demonstrate that the start point of translation of apr[BamP] in vivo is probably at codon -107, codon -103 (AAA) was changed to an ochre (TAA) by site-directed mutagenesis. Alkaline protease was produced from this ochre mutant derivative of apr[BamP] only when the host strain was Su+. The presence of a pro sequence may be common to all of the secreted proteases from bacilli.

Bibliography

Vasantha, N., Thompson, L. D., Rhodes, C., Banner, C., Nagle, J., & Filpula, D. (1984). Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. Journal of Bacteriology, 159(3), 811–819.

Authors 6
  1. N Vasantha (first)
  2. L D Thompson (additional)
  3. C Rhodes (additional)
  4. C Banner (additional)
  5. J Nagle (additional)
  6. D Filpula (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 1 p.m.)
Deposited 4 years, 2 months ago (June 12, 2021, 10:13 p.m.)
Indexed 1 year ago (Aug. 13, 2024, 3:32 p.m.)
Issued 40 years, 11 months ago (Sept. 1, 1984)
Published 40 years, 11 months ago (Sept. 1, 1984)
Published Print 40 years, 11 months ago (Sept. 1, 1984)
Funders 0

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@article{Vasantha_1984, title={Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein}, volume={159}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.159.3.811-819.1984}, DOI={10.1128/jb.159.3.811-819.1984}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Vasantha, N and Thompson, L D and Rhodes, C and Banner, C and Nagle, J and Filpula, D}, year={1984}, month=sep, pages={811–819} }