Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri
10.1128/jb.158.1.231-237.1984
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Carbon monoxide-dependent production of H2, CO2, and CH4 was detected in crude cell extracts of acetate-grown Methanosarcina barkeri. This metabolic transformation was associated with an active methyl viologen-linked CO dehydrogenase activity (5 to 10 U/mg of protein). Carbon monoxide dehydrogenase activity was inhibited 85% by 10 microM KCN and was rapidly inactivated by O2. The enzyme was nearly homogeneous after 20-fold purification, indicating that a significant proportion of soluble cell protein was CO dehydrogenase (ca. 5%). The native purified enzyme displayed a molecular weight of 232,000 and a two-subunit composition of 92,000 and 18,000 daltons. The enzyme was shown to contain nickel by isolation of radioactive CO dehydrogenase from cells grown in 63Ni. Analysis of enzyme kinetic properties revealed an apparent Km of 5 mM for CO and a Vmax of 1,300 U/mg of protein. The spectral properties of the enzyme were similar to those published for CO dehydrogenase from acetogenic anaerobes. The physiological functions of the enzyme are discussed.

Bibliography

Krzycki, J. A., & Zeikus, J. G. (1984). Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri. Journal of Bacteriology, 158(1), 231–237.

Authors 2
  1. J A Krzycki (first)
  2. J G Zeikus (additional)
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Dates
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Created 5 years, 8 months ago (Jan. 3, 2020, 12:56 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:34 p.m.)
Indexed 1 year, 7 months ago (Jan. 14, 2024, 4:31 a.m.)
Issued 41 years, 5 months ago (April 1, 1984)
Published 41 years, 5 months ago (April 1, 1984)
Published Print 41 years, 5 months ago (April 1, 1984)
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@article{Krzycki_1984, title={Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri}, volume={158}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.158.1.231-237.1984}, DOI={10.1128/jb.158.1.231-237.1984}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Krzycki, J A and Zeikus, J G}, year={1984}, month=apr, pages={231–237} }