D-erythrose supports nitrogenase activity in isolated Anabaena sp. strain 7120 heterocysts
10.1128/jb.157.2.350-356.1984
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Among organic compounds tested for their ability to support nitrogenase activity in isolated heterocysts of Anabaena sp. strain 7120 under argon, D-erythrose (5 mM) was unique in supporting acetylene reduction at 10 times the control rates. Higher concentrations of D-erythrose exhibited substrate inhibition. At 50 kPa of H2, all concentrations of D-erythrose inhibited H2-supported acetylene reduction. The effects of D-erythrose on nitrogenase activity were explored. Erythrose enhanced 15N2 incorporation by heterocysts, but NADP+ did not enhance erythrose-supported acetylene reduction. H2 protected nitrogenase from O2 inactivation, but erythrose did not; erythrose did not counter protection by H2. Tests with inhibitors of electron transport showed that erythrose-supported acetylene reduction requires electron flow through ferredoxin, a b-type cytochrome, and a 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone-sensitive transfer agent whose electron flow is not mediated through the plastoquinone and Rieske iron protein.

Bibliography

Privalle, L. S., & Burris, R. H. (1984). D-erythrose supports nitrogenase activity in isolated Anabaena sp. strain 7120 heterocysts. Journal of Bacteriology, 157(2), 350–356.

Authors 2
  1. L S Privalle (first)
  2. R H Burris (additional)
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Dates
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Created 5 years, 8 months ago (Jan. 3, 2020, 12:55 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:33 p.m.)
Indexed 1 year, 2 months ago (June 16, 2024, 7:34 p.m.)
Issued 41 years, 7 months ago (Feb. 1, 1984)
Published 41 years, 7 months ago (Feb. 1, 1984)
Published Print 41 years, 7 months ago (Feb. 1, 1984)
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@article{Privalle_1984, title={D-erythrose supports nitrogenase activity in isolated Anabaena sp. strain 7120 heterocysts}, volume={157}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.157.2.350-356.1984}, DOI={10.1128/jb.157.2.350-356.1984}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Privalle, L S and Burris, R H}, year={1984}, month=feb, pages={350–356} }