Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae
10.1128/jb.156.1.19-29.1983
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

DNA derived from the methanogenic archaebacterium Methanococcus voltae was digested with PstI restriction endonuclease and cloned into the PstI site of pBR322. The recombinant plasmids generated were used to transform a multiply auxotrophic strain of Escherichia coli with selection for tetracycline resistance. Plasmids complementing the argG(pAW1) or hisA(pAW2) mutations were isolated and characterized. Nick-translated pAW1 and pAW2 hybridized to the predicted M. voltae PstI fragments but not to digested E. coli DNA. A novel 55,000-dalton protein was synthesized in UV-irradiated cells by pAW1, whereas pAW2 synthesized a novel 26,000-dalton protein. Derivatives of pAW1 carrying insertion elements no longer complemented the argG mutation and failed to produce the 55,000-dalton protein. When an AccI fragment was deleted from pAW2, complementation of hisA did not occur and no 26,000-dalton protein was synthesized. The effect of orientation of the cloned DNA within the vector on complementation and polypeptide synthesis was examined.

Bibliography

Wood, A. G., Redborg, A. H., Cue, D. R., Whitman, W. B., & Konisky, J. (1983). Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae. Journal of Bacteriology, 156(1), 19–29.

Authors 5
  1. A G Wood (first)
  2. A H Redborg (additional)
  3. D R Cue (additional)
  4. W B Whitman (additional)
  5. J Konisky (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 12:51 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:39 p.m.)
Indexed 1 year, 7 months ago (Jan. 26, 2024, 12:27 a.m.)
Issued 41 years, 11 months ago (Oct. 1, 1983)
Published 41 years, 11 months ago (Oct. 1, 1983)
Published Print 41 years, 11 months ago (Oct. 1, 1983)
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@article{Wood_1983, title={Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae}, volume={156}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.156.1.19-29.1983}, DOI={10.1128/jb.156.1.19-29.1983}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wood, A G and Redborg, A H and Cue, D R and Whitman, W B and Konisky, J}, year={1983}, month=oct, pages={19–29} }