DOI: 10.1128/jb.156.1.186-191.1983. Protein synthesis by intact Coxiella burnetii cells

Protein synthesis by intact Coxiella burnetii cells

10.1128/jb.156.1.186-191.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Coxiella burnetii was isolated from persistently infected fibroblast host cells by a rapid mechanical lysis technique. Macromolecular synthesis was initiated in these otherwise dormant cells by incubation at pH 4.5. The synthesis of protein proceeded for as long as 24 h. Initiation of protein synthesis in C. burnetii was dependent upon RNA synthesis. Approximately 24 species of polypeptides were synthesized, and some of these appeared to be major synthetic products. Increases in protein biomass of 15 to 30% were calculated to occur during incubation. Inhibition of DNA synthesis affected protein synthesis after 12 h of incubation. The results suggest that although these parasitic bacteria did not grow in the axenic media devised, significant biosynthetic processes occurred.

Bibliography

Zuerner, R. L., & Thompson, H. A. (1983). Protein synthesis by intact Coxiella burnetii cells. Journal of Bacteriology, 156(1), 186â191.

Authors 2

R L Zuerner (first)
H A Thompson (additional)

References 20 Referenced 29

Bonner , W. M. , and R. A. Laskey . 1974 . A film detection method for tritium labeled proteins and nucleic acids in polyacrylamide gels . Eur. J. Biochem. 46 : 83 - 88 . (10.1111/j.1432-1033.1974.tb03599.x) / Eur. J. Biochem. / A film detection method for tritium labeled proteins and nucleic acids in polyacrylamide gels by Bonner W. M. (1974)
Burton , P. R. , J. Stueckemann , R. M. Welsh , and D. Paretsky . 1978 . Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells . Infect. Immun. 21 : 556 - 566 . (10.1128/iai.21.2.556-566.1978) / Infect. Immun. / Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells by Burton P. R. (1978)
Chen , T. R. 1977 . In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 Stain. Exp. Cell. Res. 104 : 255 - 262 . (10.1016/0014-4827(77)90089-1) / Stain. Exp. Cell. Res. / In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 by Chen T. R. (1977)
De Duve C. 1978. An integrated view of Iysosome function p. 25-38. In R. D. Berlin H. Herrmann I. H. Lepow and J. M. Tanzer (ed.) Molecular basis of biological degradative processes. Academic Press Inc. New York. (10.1016/B978-0-12-092150-8.50006-0)
Donahue , J. P. , and H. A. Thompson . 1980 . Protein synthesis in cell-free extracts of Coxiella burnetii . J. Gen. Microbiol. 121 : 293 - 302 . / J. Gen. Microbiol. / Protein synthesis in cell-free extracts of Coxiella burnetii by Donahue J. P. (1980)
Hackstadt T. and J. C. Williams. 1981. Incorporation of macromolecular precursors by Coxiella burnetii in an axenic medium p. 431-440. In W. Burgdorfer and R. L. Anacker (ed.) Rickettsiae and rickettsial diseases. Academic Press Inc. New York.
Hackstadt , T. , and J. C. Williams . 1981 . Biochemical stratgem for obligate parasitism of eukaryotic cells by Coxiella burnetii . Proc. Natl. Acad. Sci. U.S.A. 78 : 3240 .- 3244. (10.1073/pnas.78.5.3240) / Proc. Natl. Acad. Sci. U.S.A. / Biochemical stratgem for obligate parasitism of eukaryotic cells by Coxiella burnetii by Hackstadt T. (1981)
Hackstadt , T. , and J. C. Williams . 1981 . Stability of the adenosine 5'-triphosphate pool in Coxiella burnetii: influence of pH and substrate . J. Bacteriol. 148 : 419 - 425 . (10.1128/jb.148.2.419-425.1981) / J. Bacteriol. / Stability of the adenosine 5'-triphosphate pool in Coxiella burnetii: influence of pH and substrate by Hackstadt T. (1981)
Khavkin , T. , V. Sukhinin , and N. Amosenkova . 1981 . Host-parasite interaction and development of infraforms in chicken embryos infected with Coxiella burnetii via the yolk sac . Infect. Immun. 32 : 1281 - 1291 . (10.1128/IAI.32.3.1281-1291.1981) / Infect. Immun. / Host-parasite interaction and development of infraforms in chicken embryos infected with Coxiella burnetii via the yolk sac by Khavkin T. (1981)
Laemmll , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 27 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmll U. K. (1970)
Lin , T. , and R. Losick . 1976 . The program of protein synthesis during sporulation in Bacillus subtilis . Cell 8 : 102 - 114 . / Cell / The program of protein synthesis during sporulation in Bacillus subtilis by Lin T. (1976)
Mans , R. J. , and G. D. Novelli . 1960 . A convenient rapid and sensitive method of measuring the incorporation of radioactive amino acids into protein . Biochem. Biophys. Res. Commun. 3 : 540 - 543 . (10.1016/0006-291X(60)90171-6) / Biochem. Biophys. Res. Commun. / A convenient rapid and sensitive method of measuring the incorporation of radioactive amino acids into protein by Mans R. J. (1960)
McCaul , T. F. , and J. C. Willams . 1981 . Developmental cycle of Coxiella burnetii: structure and morphogenesis of vegetative and sporogenic differentiations . J. Bacteriol. 147 : 1063 - 1076 . (10.1128/jb.147.3.1063-1076.1981) / J. Bacteriol. / Developmental cycle of Coxiella burnetii: structure and morphogenesis of vegetative and sporogenic differentiations by McCaul T. F. (1981)
Medelson , N. H. , and J. D. Gross . 1967 . Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication . J. Bacteriol. 94 : 1603 - 1608 . (10.1128/JB.94.5.1603-1608.1967) / J. Bacteriol. / Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication by Medelson N. H. (1967)
Myers , W. F. , 0. G. Baca , and C. L. Wleman , Jr. 1980 . Genome size of the rickettsia Coxiella burnetii . J. Bacteriol. 144 : 460 - 461 . (10.1128/jb.144.1.460-461.1980) / J. Bacteriol. / Genome size of the rickettsia Coxiella burnetii by Myers W. F. (1980)
Patterson , M. K. , Jr. 1979 . Measurement of growth and viability of cells in culture . Methods Enzymol. 58 : 141 - 152 . (10.1016/S0076-6879(79)58132-4) / Methods Enzymol. / Measurement of growth and viability of cells in culture by Patterson M. K. (1979)
Reed , L. J. , and H. Muench . 1938 . A simple method of estimating fifty per cent endpoints . Am. J. Hyg. 27 : 493 - 497 . / Am. J. Hyg. / A simple method of estimating fifty per cent endpoints by Reed L. J. (1938)
Silverman , D. J. , P. Flet , and C. L. Wisseman , Jr. 1979 . Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions . J. Clin. Microbiol. 9 : 437 - 440 . (10.1128/jcm.9.3.437-440.1979) / J. Clin. Microbiol. / Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions by Silverman D. J. (1979)
Torriani , A. , and C. Levinthal . 1967 . Ordered synthesis or proteins during outgrowth of spores of Bacillus cereus . J. Bacteriol. 94 : 176 - 183 . (10.1128/JB.94.1.176-183.1967) / J. Bacteriol. / Ordered synthesis or proteins during outgrowth of spores of Bacillus cereus by Torriani A. (1967)
Wiliams , J. C. , M. G. Peacock , and T. F. McCaul . 1981 . Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components . Infect. Immun. 32 : 840 - 851 . (10.1128/IAI.32.2.840-851.1981) / Infect. Immun. / Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components by Wiliams J. C. (1981)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11:50 a.m.)
Deposited	4 years, 3 months ago (June 3, 2021, 10:54 p.m.)
Indexed	1 year, 10 months ago (Oct. 13, 2023, 8:03 p.m.)
Issued	41 years, 11 months ago (Oct. 1, 1983)
Published	41 years, 11 months ago (Oct. 1, 1983)
Published Print	41 years, 11 months ago (Oct. 1, 1983)

Funders 0

None

BibTeX

@article{Zuerner_1983, title={Protein synthesis by intact Coxiella burnetii cells}, volume={156}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.156.1.186-191.1983}, DOI={10.1128/jb.156.1.186-191.1983}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Zuerner, R L and Thompson, H A}, year={1983}, month=oct, pages={186–191} }

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