DOI: 10.1128/jb.154.2.1021-1026.1983. Low Levels of RNase H Activity in <i>Escherichia coli</i> FB2 <i>rnh</i> Result from a Single-Base Change in the Structural Gene of RNase H

Low Levels of RNase H Activity in Escherichia coli FB2 rnh Result from a Single-Base Change in the Structural Gene of RNase H

10.1128/jb.154.2.1021-1026.1983

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The DNA coding for RNase H from a mutant strain of Escherichia coli (FB2) was cloned into plasmid pBR322. DNA sequence analysis and the exchange of a portion of the mutant and wild-type genes revealed that a single-base alteration (C→T) in the coding region of the structural gene for RNase H is responsible for the difference in RNase H activity of the wild-type and mutant cells.

Bibliography

Kanaya, S., & Crouch, R. J. (1983). Low Levels of RNase H Activity in Escherichia coli FB2 rnh Result from a Single-Base Change in the Structural Gene of RNase H. Journal of Bacteriology, 154(2), 1021â1026.

Authors 2

Shigenori Kanaya (first)
Robert J. Crouch (additional)

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 12:43 p.m.)
Deposited	4 years, 3 months ago (May 28, 2021, 3:38 p.m.)
Indexed	1 year, 11 months ago (Sept. 16, 2023, 1:54 a.m.)
Issued	42 years, 3 months ago (May 1, 1983)
Published	42 years, 3 months ago (May 1, 1983)
Published Print	42 years, 3 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Kanaya_1983, title={Low Levels of RNase H Activity in Escherichia coli FB2 rnh Result from a Single-Base Change in the Structural Gene of RNase H}, volume={154}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.154.2.1021-1026.1983}, DOI={10.1128/jb.154.2.1021-1026.1983}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kanaya, Shigenori and Crouch, Robert J.}, year={1983}, month=may, pages={1021–1026} }

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