Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli
10.1128/jb.153.3.1424-1431.1983
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A clone bearing the structural gene sacB, coding for the exoenzyme levansucrase, was isolated from a library of Bacillus subtilis DNA that was cloned in phage lambda charon 4A on the basis of the transforming activity of the chimeric DNA. This lambda clone also was found to contain the sacR and smo loci. Subcloning the sacB-sacR region in plasmid pBR325 resulted in a clone which directed levansucrase synthesis in Escherichia coli. The nucleotide sequence coding for the secreted protein was localized on the physical map of the cloned DNA.

Bibliography

Gay, P., Le Coq, D., Steinmetz, M., Ferrari, E., & Hoch, J. A. (1983). Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli. Journal of Bacteriology, 153(3), 1424–1431.

Authors 5
  1. P Gay (first)
  2. D Le Coq (additional)
  3. M Steinmetz (additional)
  4. E Ferrari (additional)
  5. J A Hoch (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 12:42 p.m.)
Deposited 4 years, 2 months ago (May 31, 2021, 7:56 p.m.)
Indexed 3 weeks, 4 days ago (Aug. 5, 2025, 8:47 a.m.)
Issued 42 years, 5 months ago (March 1, 1983)
Published 42 years, 5 months ago (March 1, 1983)
Published Print 42 years, 5 months ago (March 1, 1983)
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@article{Gay_1983, title={Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli}, volume={153}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.153.3.1424-1431.1983}, DOI={10.1128/jb.153.3.1424-1431.1983}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Gay, P and Le Coq, D and Steinmetz, M and Ferrari, E and Hoch, J A}, year={1983}, month=mar, pages={1424–1431} }