DOI: 10.1128/jb.150.3.1430-1437.1982. "Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus

"Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus

10.1128/jb.150.3.1430-1437.1982

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

During fruiting-body formation in Myxococcus xanthus, cells aggregate into raised mounds, where they sporulate. A new class of aggregation-defective developmental mutants was identified within a collection of nonfruiting mutants of M. xanthus. The mutants failed to aggregate into discrete mounds, but rather aggregated into "frizzy" filaments. Many cells within the filaments sporulated normally. Pairwise mixtures of representative frizzy mutants were unable to stimulate each other to aggregate normally. Two strains of M. xanthus were isolated which contained transposon Tn5 insertions mapping near one frizzy mutation. A search through 36 mutants exhibiting the frizzy phenotype showed that all were linked to the same Tn5 insertion sites. Three-factor cross-analysis of 22 of these mutants allowed the mapping of these mutations into many loci. The localization of Tn5 inserts adjacent to this region make possible further manipulation of these genes.

Bibliography

Zusman, D. R. (1982). âFrizzyâ mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus. Journal of Bacteriology, 150(3), 1430â1437.

Authors 1

D R Zusman (first)

References 15 Referenced 63

Berg D. E. 1977. Insertion and excision of the transposable kanamycin resistance determinant TnS p. 205-212. In A. I. Bukhari J. Shapiro and S. L. Adhya (ed.) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Brandsma , J. A. , C. A. van Sluis , and P. van de Putte . 1981 . Use of transposons in cloning poorly selectable genes of Escherichia coli: cloning of uvrA and adjacent genes . J Bacteriol. 147 : 682 - 684 . (10.1128/jb.147.2.682-684.1981) / J Bacteriol. / Use of transposons in cloning poorly selectable genes of Escherichia coli: cloning of uvrA and adjacent genes by Brandsma J. A. (1981)
Bretscher , A. P. , and D. Kaiser . 1978 . Nutrition of Myxococcus xanthus, a fruiting myxobacterium . J. Bacteriol. 133 : 763 - 768 . (10.1128/JB.133.2.763-768.1978) / J. Bacteriol. / Nutrition of Myxococcus xanthus, a fruiting myxobacterium by Bretscher A. P. (1978)
Campbell A. , D. E. Berg , D. Botstein , E. M. Lederberg , R. P. Novick , P. Starlinger , and W. Szybalski . 1979 . Nomenclature of transposable elements in prokaryotes . Gene 5 : 197 - 206 . (10.1016/0378-1119(79)90078-7) / Gene / Nomenclature of transposable elements in prokaryotes by Campbell A. (1979)
Campos , J. M. , J. Geisselsoder , and D. R. Zusman . 1978 . Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus . J. Mol. Biol. 119 : 167 - 178 . (10.1016/0022-2836(78)90431-X) / J. Mol. Biol. / Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus by Campos J. M. (1978)
Hagen , D. C. , A. P. Bretscher , and D. Kaiser . 1978 . Synergism between morphogenetic mutants of Myxococcus xanthus . Dev. Biol. 64 : 284 - 296 . (10.1016/0012-1606(78)90079-9) / Dev. Biol. / Synergism between morphogenetic mutants of Myxococcus xanthus by Hagen D. C. (1978)
Hodgkin , J. , and D. Kaiser . 1979 . Genetics of gliding motility in Myxococcus xanthus (Myxobacteriales): two gene systems control movement . Mol. Gen. Genet. 171 : 177 - 191 . (10.1007/BF00270004) / Mol. Gen. Genet. / Genetics of gliding motility in Myxococcus xanthus (Myxobacteriales): two gene systems control movement by Hodgkin J. (1979)
Kaiser , D. , C. Manoil , and M. Dworkin . 1979 . Myxobacteria: cell interactions, genetics, and development. Annu . Rev. Microbiol. 33 : 595 - 639 . (10.1146/annurev.mi.33.100179.003115) / Rev. Microbiol. / Myxobacteria: cell interactions, genetics, and development. Annu by Kaiser D. (1979)
Kuner , J. , and D. Kaiser . 1981 . Introduction of transposon TnS into Myxococcus for analysis of developmental and other non-selectable mutants . Proc. Natl. Acad. Sci. U.S.A. 78 : 425 - 429 . (10.1073/pnas.78.1.425) / Proc. Natl. Acad. Sci. U.S.A. / Introduction of transposon TnS into Myxococcus for analysis of developmental and other non-selectable mutants by Kuner J. (1981)
Manoil , C. , and D. Kaiser . 1980 . Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus xanthus fruiting body development . J. Bacteriol. 141 : 305 - 315 . (10.1128/JB.141.1.305-315.1980) / J. Bacteriol. / Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus xanthus fruiting body development by Manoil C. (1980)
Morrison , C. E. , and D. R. Zusman . 1979 . Myxococcus xanthus mutants with temperature-sensitive, stage-specific defects: evidence for independent pathways in development . J. Bacteriol. 140 : 1036 - 1042 . (10.1128/JB.140.3.1036-1042.1979) / J. Bacteriol. / Myxococcus xanthus mutants with temperature-sensitive, stage-specific defects: evidence for independent pathways in development by Morrison C. E. (1979)
Torti , S. , and D. R. Zusman . 1981 . Genetic characterization of aggregation-defective developmental mutants of Myxococcus xanthus . J. Bacteriol. 147 : 768 - 775 . (10.1128/jb.147.3.768-775.1981) / J. Bacteriol. / Genetic characterization of aggregation-defective developmental mutants of Myxococcus xanthus by Torti S. (1981)
Wireman , J. W. , and M. Dworkin . 1977 . Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus . J. Bacteriol. 129 : 796 - 802 . (10.1128/JB.129.2.798-802.1977) / J. Bacteriol. / Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus by Wireman J. W. (1977)
Wu , T. T. 1966 . A model for three-point analysis of random general transduction . Genetics 54 : 405 - 410 . (10.1093/genetics/54.2.405) / Genetics / A model for three-point analysis of random general transduction by Wu T. T. (1966)
Zusman D. R. 1980. Genetic approaches to the study of development in the myxobacteria p. 41-78. In T. Leighton and W. F. Loomis (ed.) The molecular genetics of development. Academic Press Inc. New York.

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 12:34 p.m.)
Deposited	4 years, 3 months ago (May 27, 2021, 8:15 p.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:43 a.m.)
Issued	43 years, 3 months ago (June 1, 1982)
Published	43 years, 3 months ago (June 1, 1982)
Published Print	43 years, 3 months ago (June 1, 1982)

Funders 0

None

BibTeX

@article{Zusman_1982, title={“Frizzy” mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus}, volume={150}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.150.3.1430-1437.1982}, DOI={10.1128/jb.150.3.1430-1437.1982}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Zusman, D R}, year={1982}, month=jun, pages={1430–1437} }

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