Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis
10.1128/jb.148.3.877-883.1981
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A 44-megadalton plasmid associated with virulence and Ca2+ dependence from Yersinia enterocolitica 8081 was compared at the molecular level with a 47-megadalton plasmid associated with Ca2+ dependence from Yersinia pestis EV76. The plasmids were found to share 55% deoxyribonucleic acid sequence homology distributed over approximately 80% of the plasmid genomes. One region in which the plasmids differed was found to contain sequences concerned with essential plasmid functions. Forty-five mutants of Y. pestis were isolated which had spontaneously acquired the ability to grow on calcium-free medium (Ca2+ independence). Of these mutants, 21 were cured of their 47-megadalton plasmid, whereas the remaining had either suffered a major deletion of the plasmid or had a 2.2-kilobase insertion located in one of two adjacent BamHI restriction fragments encompassing approximately 9 kilobases. The inserted sequence was found at numerous sites on the Y. pestis chromosome and on all three plasmids in the strain and may represent a Y. pestis insertion sequence element.

Bibliography

Portnoy, D. A., & Falkow, S. (1981). Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. Journal of Bacteriology, 148(3), 877–883.

Authors 2
  1. D A Portnoy (first)
  2. S Falkow (additional)
References 28 Referenced 186
  1. Ben-Gurion , R. , and A. Shafferman . 1981 . Essential virulence determinants of different Yersinia species are carried on a common plasmid . Plasmid 5 : 183 - 187 . (10.1016/0147-619X(81)90019-6) / Plasmid / Essential virulence determinants of different Yersinia species are carried on a common plasmid by Ben-Gurion R. (1981)
  2. Bercovier , H. , H. H. Mollaret , J. M. Alonso , J. Brault , G. R. Fanning , A. G. Steigerwalt , and D. J. Brenner . 1980 . Intra- and interspecies relatedness of Yersinia pestis by DNA hybridization and its relationship to Yersinia pseudotuberculosis . Curr. Microbiol. 4 : 225 - 229 . (10.1007/BF02605861) / Curr. Microbiol. / Intra- and interspecies relatedness of Yersinia pestis by DNA hybridization and its relationship to Yersinia pseudotuberculosis by Bercovier H. (1980)
  3. Bottone , E. J. 1977 . Yersinia enterocolitica: a panoramic view of a charismatic microorganism . Crit. Rev. Microbiol. 5 : 211 - 241 . (10.3109/10408417709102312) / Crit. Rev. Microbiol. / Yersinia enterocolitica: a panoramic view of a charismatic microorganism by Bottone E. J. (1977)
  4. Brenner , D. J. , G. R. Fanning , K. E. Johnson , R. V. Citarella , and S. Falkow . 1969 . Polynucleotide sequence relationships among members of Enterobacteriaceae . J. Bacteriol. 98 : 637 - 650 . (10.1128/JB.98.2.637-650.1969) / J. Bacteriol. / Polynucleotide sequence relationships among members of Enterobacteriaceae by Brenner D. J. (1969)
  5. Brenner , D. J. , A. G. Steigerwalt , D. P. Falcao , R. E. Weaver , and G. R. Fanning . 1976 . Characterization of Yersinia enterocolitica and Yersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions . Int. J. Syst. Bacteriol. 26 : 180 - 194 . (10.1099/00207713-26-2-180) / Int. J. Syst. Bacteriol. / Characterization of Yersinia enterocolitica and Yersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions by Brenner D. J. (1976)
  6. Brubaker , R. R. 1972 . The genus Yersinia: biochemistry and genetics of virulence . Curr. Top. Microbiol. 37 : 111 - 158 . / Curr. Top. Microbiol. / The genus Yersinia: biochemistry and genetics of virulence by Brubaker R. R. (1972)
  7. Brubaker R. R. 1979. Expression of virulence in Yersiniae p. 168-171. In D. Schlessinger (ed.) Microbiology-1979. American Society for Microbiology Washington D.C.
  8. Brubaker , R. R. , and M. J. Surgala . 1962 . Genotypic alterations associated with avirulence in streptomycinresistant Pasteurella pestis . J. Bacteriol. 84 : 615 - 624 . (10.1128/JB.84.4.615-624.1962) / J. Bacteriol. / Genotypic alterations associated with avirulence in streptomycinresistant Pasteurella pestis by Brubaker R. R. (1962)
  9. Carter , P. B. , R. J. Zahorchak , and R. R. Brubaker . 1980 . Plague virulence antigens from Yersinia enterocolitica . Infect. Immun. 28 : 638 - 640 . (10.1128/iai.28.2.638-640.1980) / Infect. Immun. / Plague virulence antigens from Yersinia enterocolitica by Carter P. B. (1980)
  10. Devenish , J. A. , and D. A. Schiemann . 1981 . HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity . Infect. Immun. 32 : 48 - 55 . (10.1128/IAI.32.1.48-55.1981) / Infect. Immun. / HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity by Devenish J. A. (1981)
  11. Ferber , D. M. , and R. R. Brubaker . 1981 . Plasmids in Yersinia pestis . Infect. Immun. 31 : 839 841. (10.1128/IAI.31.2.839-841.1981) / Infect. Immun. / Plasmids in Yersinia pestis by Ferber D. M. (1981)
  12. Gemski , P. , J. R. Lazere , and T. Casey . 1980 . Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica . Infect. Immun. 27 : 682 - 685 . (10.1128/IAI.27.2.682-685.1980) / Infect. Immun. / Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica by Gemski P. (1980)
  13. Gemski , P. , J. R. Lazere , T. Casey , and J. A Wohlhieter . 1980 . Presence ofa virulence-associated plasmid in Yersinia pseudotuberculosis . Infect. Immun. 28 : 1044 - 1047 . (10.1128/iai.28.3.1044-1047.1980) / Infect. Immun. / Presence ofa virulence-associated plasmid in Yersinia pseudotuberculosis by Gemski P. (1980)
  14. Higuchi , K. , and J. L Smith . 1961 . Studies on the nutrition and physiology of Pasteurella pestis. IV. A differential plating medium for the estimation of the mutation rate to avirulence . J. Bacteriol. 81 : 605 - 608 . (10.1128/jb.81.4.605-608.1961) / J. Bacteriol. / Studies on the nutrition and physiology of Pasteurella pestis. IV. A differential plating medium for the estimation of the mutation rate to avirulence by Higuchi K. (1961)
  15. Janssen , W. A. , and Mo J. Surgalla . 1969 . Plague bacillus: survival within host phagocytes . Science 163 : 950 - 952 . (10.1126/science.163.3870.950) / Science / Plague bacillus: survival within host phagocytes by Janssen W. A. (1969)
  16. Kleckner , N. , J. Roth , and D. Botstein . 1977 . Genetic engineering in vivo using tranalocatable drug-resistance elements . J. Mol. Biol. 116 : 125 - 159 . (10.1016/0022-2836(77)90123-1) / J. Mol. Biol. / Genetic engineering in vivo using tranalocatable drug-resistance elements by Kleckner N. (1977)
  17. Lee , W. H. , P. P. McGrath , P. H. Carter , and E. L. Eide . 1977 . The ability of some Yersinia enterocolitica strains to invade HeLa cells . Can. J. Microbiol. 23 : 1714 - 1722 . (10.1139/m77-247) / Can. J. Microbiol. / The ability of some Yersinia enterocolitica strains to invade HeLa cells by Lee W. H. (1977)
  18. Maniatis , T. , A. Jeffrey , and D. G. Kleid . 1975 . Nucleotide sequence of the rightward operator of phage lambda . Proc. Natl. Acad. Sci. U.S.A. 72 : 1184 - 1188 . (10.1073/pnas.72.3.1184) / Proc. Natl. Acad. Sci. U.S.A. / Nucleotide sequence of the rightward operator of phage lambda by Maniatis T. (1975)
  19. Meyers , J. A. , D. Sanchez , L P . Elwell , and S. Falkow . 1976 . Simple agarose gel electrophoretic.method for the identification and characterization of plasmid deoxyribonucleic acid . J. Bacteriol. 127 : 1529 - 1537 . (10.1128/JB.127.3.1529-1537.1976) / J. Bacteriol. / Simple agarose gel electrophoretic.method for the identification and characterization of plasmid deoxyribonucleic acid by Meyers J. A. (1976)
  20. Nyman , K. , K. Nakamara , H. Ohtsubo , and E. Ohtsubo . 1981 . Distribution of the insertion sequence Isl in gram-negative bacteria . Nature (London) 289 : 609 - 612 . (10.1038/289609a0) / Nature (London) / Distribution of the insertion sequence Isl in gram-negative bacteria by Nyman K. (1981)
  21. Portnoy , D. A. , S. L. Moseley , and S. Falkow . 1981 . Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis . Infect. Immun. 31 : 775 - 782 . (10.1128/IAI.31.2.775-782.1981) / Infect. Immun. / Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis by Portnoy D. A. (1981)
  22. Shapiro , J. A. 1979 . Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements . Proc. Natl. Acad. Sci. U.S.A. 76 : 1933 - 1937 . (10.1073/pnas.76.4.1933) / Proc. Natl. Acad. Sci. U.S.A. / Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements by Shapiro J. A. (1979)
  23. Silver 5. 1977. Calcium transport in microorganisms p. 49-101. In E. D. Weinberg (ed.) Microoganisms and minerals. Marcel Dekker Inc. New York.
  24. Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 . (10.1016/S0022-2836(75)80083-0) / J. Mol. Biol. / Detection of specific sequences among DNA fragments separated by gel electrophoresis by Southern E. M. (1975)
  25. Straley , S. C. , and R. R. Brubaker . 1981 . Cytoplasmic and membrane proteins of Yersiniae cultivated under conditions simulating mammalian intracellular environment . Proc. Natl. Acad. Sci. U.S.A. 78 : 1224 - 1228 . (10.1073/pnas.78.2.1224) / Proc. Natl. Acad. Sci. U.S.A. / Cytoplasmic and membrane proteins of Yersiniae cultivated under conditions simulating mammalian intracellular environment by Straley S. C. (1981)
  26. Une , T. 1977 . Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro . Microbiol. Immunol. 21 : 365 - 377 . (10.1111/j.1348-0421.1977.tb00301.x) / Microbiol. Immunol. / Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro by Une T. (1977)
  27. Wahl , G. M. , M. Stern , and G. R. Stark . 1979 . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization using dextran sulfate . Proc. Natl. Acad. Sci. U.S.A. 76 : 3683 - 3687 . (10.1073/pnas.76.8.3683) / Proc. Natl. Acad. Sci. U.S.A. / Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization using dextran sulfate by Wahl G. M. (1979)
  28. Zink , D. L , J. C. Feeley , J. G. Wells , C. Vanderzant , J. C. Vickery , W. D. Roof , and G. A. O'Donovan . 1980 . Plasmid-mediated tissue invasiveness in Yersinia enterocolitica . Nature (London) 283 : 224 - 226 . (10.1038/283224a0) / Nature (London) / Plasmid-mediated tissue invasiveness in Yersinia enterocolitica by Zink D. L (1980)
Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 11:41 a.m.)
Deposited 2 years, 10 months ago (Oct. 9, 2022, 5:59 p.m.)
Indexed 2 months, 2 weeks ago (June 4, 2025, 6:32 a.m.)
Issued 43 years, 8 months ago (Dec. 1, 1981)
Published 43 years, 8 months ago (Dec. 1, 1981)
Published Print 43 years, 8 months ago (Dec. 1, 1981)
Funders 0

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@article{Portnoy_1981, title={Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis}, volume={148}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.148.3.877-883.1981}, DOI={10.1128/jb.148.3.877-883.1981}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Portnoy, D A and Falkow, S}, year={1981}, month=dec, pages={877–883} }