DOI: 10.1128/jb.147.2.382-389.1981. Defective enzyme II-BGlc of the phosphoenolpyruvate:sugar phosphotransferase system leading to uncoupling of transport and phosphorylation in Salmonella typhimurium

Defective enzyme II-BGlc of the phosphoenolpyruvate:sugar phosphotransferase system leading to uncoupling of transport and phosphorylation in Salmonella typhimurium

10.1128/jb.147.2.382-389.1981

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Transport and phosphorylation of glucose via enzymes II-A/II-B and II-BGlc of the phosphoenolpyruvate:sugar phosphotransferase system are tightly coupled in Salmonella typhimurium. Mutant strains (pts) that lack the phosphorylating proteins of this system, enzyme I and HPr, are unable to transport or to grow on glucose. From ptsHI deletion strains of S. typhimurium, mutants were isolated that regained growth on and transport of glucose. Several lines of evidence suggest that these Glc+ mutants have an altered enzyme II-BGlc as follows. (i) Insertion of a ptsG::Tn10 mutation (resulting in a defective II-BGlc) abolished growth on and transport of glucose in these Glc+ strains. Introduction of a ptsM mutation, on the other hand, which abolishes II-A/II-B activity, had no effect. (ii) Methyl alpha-glucoside transport and phosphorylation (specific for II-BGlc) was lowered or absent in ptsH+,I+ transductants of these Glc+ strains. Transport and phosphorylation of other phosphoenolpyurate:sugar phosphotransferase system sugars were normal. (iii) Membranes isolated from these Glc+ mutants were unable to catalyze transphosphorylation of methyl alpha-glucoside by glucose 6-phosphate, but transphosphorylation of mannose by glucose 6-phosphate was normal. (iv) The mutation was in the ptsG gene or closely linked to it. We conclude that the altered enzyme II-BGlc has acquired the capacity to transport glucose in the absence of phosphoenolpyruvate:sugar phosphotransferase system-mediated phosphorylation. However, the affinity for glucose decreased at least 1,000-fold as compared to the wild-type strain. At the same time the mutated enzyme II-BGlc lost the ability to catalyze the phosphorylation of its substrates via IIIGlc.

Bibliography

Postma, P. W. (1981). Defective enzyme II-BGlc of the phosphoenolpyruvate:sugar phosphotransferase system leading to uncoupling of transport and phosphorylation in Salmonella typhimurium. Journal of Bacteriology, 147(2), 382â389.

Authors 1

P W Postma (first)

References 25 Referenced 46

Bourd , G. I. , R. S. Erlagaeva , T. N. Bolshakova , and V. N. Gershanovitch . 1975 . Glucose catabolite repression in Escherichia coli K12 mutants defective in methyl-a-D-glucoside transport . Eur. J. Biochem. 53 : 419 - 427 . (10.1111/j.1432-1033.1975.tb04082.x) / Eur. J. Biochem. / Glucose catabolite repression in Escherichia coli K12 mutants defective in methyl-a-D-glucoside transport by Bourd G. I. (1975)
Curtiss , S. J. , and W. Epstein . 1975 . Phosphorylation of D-glucose in Escherichia coli mutants defective in glucose phosphotransferase, mannose phosphotransferase, and glucokinase . J. Bacteriol. 122 : 1189 - 1199 . (10.1128/JB.122.3.1189-1199.1975) / J. Bacteriol. / Phosphorylation of D-glucose in Escherichia coli mutants defective in glucose phosphotransferase, mannose phosphotransferase, and glucokinase by Curtiss S. J. (1975)
Davis R. W. D. Botstein and J. R. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Ely , B. , R. M. Weppelman , H. C. Massey , Jr. , and P. E. Hartman . 1974 . Some improved methods in P22 transduction . Genetics 76 : 625 - 631 . (10.1093/genetics/76.4.625) / Genetics / Some improved methods in P22 transduction by Ely B. (1974)
Erlagaeva , R. S. , T. N. Bolshakova , M. V. Shulgina , G. I. Bourd , and V. N. Gershanovitch . 1977 . Glucose effect in tgl mutant of Escherichia coli K12 defective in methyl-a-D-glucoside transport . Eur. J. Biochem. 72 : 127 - 135 . (10.1111/j.1432-1033.1977.tb11232.x) / Eur. J. Biochem. / Glucose effect in tgl mutant of Escherichia coli K12 defective in methyl-a-D-glucoside transport by Erlagaeva R. S. (1977)
Gachelin , G. 1970 . Studies on the a-methylglucoside permease of Escherichia coli. A two-step mechanism for the accumulation of a-methyl-glucoside 6-phosphate . Eur. J. Biochem. 16 : 342 - 357 . (10.1111/j.1432-1033.1970.tb01088.x) / Eur. J. Biochem. / Studies on the a-methylglucoside permease of Escherichia coli. A two-step mechanism for the accumulation of a-methyl-glucoside 6-phosphate by Gachelin G. (1970)
Kleckner , N. , J. Roth , and D. Botstein . 1977 . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics . J. Mol. Biol. 116 : 125 - 159 . (10.1016/0022-2836(77)90123-1) / J. Mol. Biol. / Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics by Kleckner N. (1977)
Kornberg , H. L. , and J. Smith . 1972 . Genetic control of glucose uptake by Escherichia coli . FEBS Lett. 20 : 270 - 272 . (10.1016/0014-5793(72)80084-X) / FEBS Lett. / Genetic control of glucose uptake by Escherichia coli by Kornberg H. L. (1972)
Kundig , W. , and S. Roseman . 1971 . Sugar transport. II. Characterization of constitutive membrane-bound Enzymes II of the Escherichia coli phosphotransferase system . J. Biol. Chem. 246 : 1407 - 1418 . / J. Biol. Chem. / Sugar transport. II. Characterization of constitutive membrane-bound Enzymes II of the Escherichia coli phosphotransferase system by Kundig W. (1971)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Melton , T. , W. Kundig , P. E. Hartman , and N. Meadow . 1976 . 3-Deoxy-3-fluoro-D-glucose-resistant Salmonella typhimurium mutants defective in the phosphoenolpyruvate:glycose phosphotransferase system . J. Bacteriol. 128 : 794 - 800 . (10.1128/JB.128.3.794-800.1976) / J. Bacteriol. / 3-Deoxy-3-fluoro-D-glucose-resistant Salmonella typhimurium mutants defective in the phosphoenolpyruvate:glycose phosphotransferase system by Melton T. (1976)
Postma , P. W. 1977 . Galactose transport in Salmonella typhimurium . J. Bacteriol. 129 : 630 - 639 . (10.1128/JB.129.2.630-639.1977) / J. Bacteriol. / Galactose transport in Salmonella typhimurium by Postma P. W. (1977)
Postma , P. W. , J. C. Cordaro , and S. Roseman . 1977 . Sugar transport. A pleiotropic membrane mutant of Salmonella typhimurium . J. Biol. Chem. 252 : 7862 - 7876 . (10.1016/S0021-9258(17)41045-3) / J. Biol. Chem. / Sugar transport. A pleiotropic membrane mutant of Salmonella typhimurium by Postma P. W. (1977)
Postma , P. W. , and S. Roseman . 1976 . The bacterial phosphoenolpyruvate: sugar phosphotransferase system . Biochim. Biophys. Acta 457 : 213 - 257 . (10.1016/0304-4157(76)90001-0) / Biochim. Biophys. Acta / The bacterial phosphoenolpyruvate: sugar phosphotransferase system by Postma P. W. (1976)
. Postma , P. W. , A. Schuitema , and C. Kwa . 1981 . Regulation of methyl ,8-galactoside permease activity in pts and crr mutants of Salmonella typhimurium . Mol. Gen. Genet. 181 : 448 - 453 . (10.1007/BF00428734) / Mol. Gen. Genet. / Regulation of methyl ,8-galactoside permease activity in pts and crr mutants of Salmonella typhimurium by Postma P. W. (1981)
Postma , P. W. , and J. B. Stock . 1980 . Enzymes II of the phosphotransferase system do not catalyze sugar transport in the absence of phosphorylation . J. Bacteriol. 141 : 476 - 484 . (10.1128/JB.141.2.476-484.1980) / J. Bacteriol. / Enzymes II of the phosphotransferase system do not catalyze sugar transport in the absence of phosphorylation by Postma P. W. (1980)
Rephaeli , A. W. , and M. H. Saier , Jr. 1978 . Kinetic analyses of the sugar phosphate: sugar transphosphorylation reaction catalyzed by the glucose enzyme II complex of the bacterial phosphotransferase system . J. Biol. Chem. 253 : 7595 - 7597 . (10.1016/S0021-9258(17)34408-3) / J. Biol. Chem. / Kinetic analyses of the sugar phosphate: sugar transphosphorylation reaction catalyzed by the glucose enzyme II complex of the bacterial phosphotransferase system by Rephaeli A. W. (1978)
Saier , M. H. , Jr. , F. G. Bromberg , and S. Roseman . 1973 . Characterization of constitutive galactose permease mutants in Salmonella typhimurium . J. Bacteriol. 113 : 512 - 514 . (10.1128/jb.113.1.512-514.1973) / J. Bacteriol. / Characterization of constitutive galactose permease mutants in Salmonella typhimurium by Saier M. H. (1973)
Saier , M. H. , Jr. , B. U. Feucht , and W. K. Mora . 1977 . Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme II complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system . J. Biol. Chem. 252 : 8899 - 8907 . (10.1016/S0021-9258(17)38325-4) / J. Biol. Chem. / Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme II complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system by Saier M. H. (1977)
Saier , M. H. , Jr. , W. S. Young , and S. Roseman . 1971 . Utilization and transport of hexoses by mutant strains of Salmonella typhimurium lacking Enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system . J. Biol. Chem. 246 : 5838 - 5840 . (10.1016/S0021-9258(18)61883-6) / J. Biol. Chem. / Utilization and transport of hexoses by mutant strains of Salmonella typhimurium lacking Enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system by Saier M. H. (1971)
Sanderson , K. E. , and P. E. Hartman . 1978 . Linkage map of Salmonella typhimurium, edition V . Microbiol. Rev. 42 : 471 - 519 . (10.1128/MR.42.2.471-519.1978) / Microbiol. Rev. / Linkage map of Salmonella typhimurium, edition V by Sanderson K. E. (1978)
Scholte , B. J. , and P. W. Postma . 1981 . Competition between two pathways for sugar uptake by the phosphoenolpyruvate-dependent sugar phosphotransferase system in Salmonella typhimurium . Eur. J. Biochem. 114 : 51 - 58 . (10.1111/j.1432-1033.1981.tb06171.x) / Eur. J. Biochem. / Competition between two pathways for sugar uptake by the phosphoenolpyruvate-dependent sugar phosphotransferase system in Salmonella typhimurium by Scholte B. J. (1981)
Waygood , E. B. , N. D. Meadow , and S. Roseman . 1979 . Modified assay procedures for the phosphotransferase system in enteric bacteria . Anal. Biochem. 95 : 293 - 304 . (10.1016/0003-2697(79)90219-7) / Anal. Biochem. / Modified assay procedures for the phosphotransferase system in enteric bacteria by Waygood E. B. (1979)
West , I. C. , and T. H. Wilson . 1973 . Galactoside transport dissociated from proton movement in mutants of Escherichia coli . Biochem. Biophys. Res. Commun. 50 : 551 - 558 . (10.1016/0006-291X(73)90875-9) / Biochem. Biophys. Res. Commun. / Galactoside transport dissociated from proton movement in mutants of Escherichia coli by West I. C. (1973)
Wilson , T. H. , and M. Kusch . 1972 . A mutant of Escherichia coli K12 energy-uncoupled for lactose transport . Biochim. Biophys. Acta 255 : 786 - 797 . (10.1016/0005-2736(72)90391-4) / Biochim. Biophys. Acta / A mutant of Escherichia coli K12 energy-uncoupled for lactose transport by Wilson T. H. (1972)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 11:40 a.m.)
Deposited	4 years, 3 months ago (May 26, 2021, 3:36 p.m.)
Indexed	1 year, 7 months ago (Jan. 26, 2024, 12:21 a.m.)
Issued	44 years, 1 month ago (Aug. 1, 1981)
Published	44 years, 1 month ago (Aug. 1, 1981)
Published Print	44 years, 1 month ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{Postma_1981, title={Defective enzyme II-BGlc of the phosphoenolpyruvate:sugar phosphotransferase system leading to uncoupling of transport and phosphorylation in Salmonella typhimurium}, volume={147}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.147.2.382-389.1981}, DOI={10.1128/jb.147.2.382-389.1981}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Postma, P W}, year={1981}, month=aug, pages={382–389} }

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      "article-title": "Glucose effect in tgl mutant of Escherichia coli K12 defective in methyl-a-D-glucoside transport",
      "volume": "72",
      "author": "Erlagaeva R. S.",
      "year": "1977",
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      "DOI": "10.1111/j.1432-1033.1970.tb01088.x",
      "article-title": "Studies on the a-methylglucoside permease of Escherichia coli. A two-step mechanism for the accumulation of a-methyl-glucoside 6-phosphate",
      "volume": "16",
      "author": "Gachelin G.",
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      "article-title": "Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics",
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