DOI: 10.1128/jb.146.2.668-675.1981. Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro

Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro

10.1128/jb.146.2.668-675.1981

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The structural gene for alkaline phosphatase (phoA) of Escherichia coli was cloned into the PstI site of pBR322, from a transducing bacteriophage, lambda p(phoA-proC). The restriction map of the plasmid was established. Based upon this information, several phoA deletion plasmids as well as a smaller phoA+ plasmid were constructed. The genetic map and restriction map were correlated by recombination analysis. Cells carrying one of the phoA+ plasmids overproduce alkaline phosphatase 10-fold upon phosphate limitation. However, both regulation and processing of the enzyme were found to be normal.

Authors 4

H Inouye (first)
S Michaelis (additional)
A Wright (additional)
J Beckwith (additional)

References 24 Referenced 99

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Chang , C. N. , H. Inouye , P. Model , and J. Beckwith . 1980 . Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation . J. Bacteriol. 142 : 726 - 728 . (10.1128/JB.142.2.726-728.1980) / J. Bacteriol. / Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation by Chang C. N. (1980)
Cohen , S. N. , A. C. Y. Chang , and H. W. Boyer . 1973 . Construction of biologically functional bacterial plasmids in vitro . Proc. Nati. Acad. Sci. U.S.A. 70 : 3240 - 3244 . (10.1073/pnas.70.11.3240) / Proc. Nati. Acad. Sci. U.S.A. / Construction of biologically functional bacterial plasmids in vitro by Cohen S. N. (1973)
Dohi , M. , H. Inouye , A. Kikuchi , and L Gorini . 1978 . In vitro synthesis of ornithine transcarbamylase . J. Biochem. (Tokyo) 84 : 1389 - 1399 . (10.1093/oxfordjournals.jbchem.a132261) / J. Biochem. (Tokyo) / In vitro synthesis of ornithine transcarbamylase by Dohi M. (1978)
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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 12:21 p.m.)
Deposited	4 years ago (July 29, 2021, 1:24 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:50 a.m.)
Issued	44 years, 3 months ago (May 1, 1981)
Published	44 years, 3 months ago (May 1, 1981)
Published Print	44 years, 3 months ago (May 1, 1981)

Funders 0

None

BibTeX

@article{Inouye_1981, title={Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro}, volume={146}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.146.2.668-675.1981}, DOI={10.1128/jb.146.2.668-675.1981}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Inouye, H and Michaelis, S and Wright, A and Beckwith, J}, year={1981}, month=may, pages={668–675} }

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