DOI: 10.1128/jb.146.1.325-330.1981. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli

Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli

10.1128/jb.146.1.325-330.1981

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Escherichia coli K-12 minicells were employed to investigate the biosynthesis of plasmid-encoded, heat-labile enterotoxin of E. coli. Two polypeptide species related to the B subunit of the toxin were expressed in the minicells. One of these polypeptides (molecular weight, 11,500) was immunoprecipitated by antiserum to cholera toxin. Because the B subunits of heat-labile enterotoxin and cholera toxin have common antigenic sites, we concluded that this species was the mature B subunit. The larger polypeptide (molecular weight, 13,000) is likely to be a precursor of the B subunit because it could be chased into the mature form. This conversion was inhibited by compounds which dissipate proton motive force, suggesting that processing requires energy.

Bibliography

Palva, E. T., Hirst, T. R., Hardy, S. J., Holmgren, J., & Randall, L. (1981). Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli. Journal of Bacteriology, 146(1), 325â330.

Authors 5

E T Palva (first)
T R Hirst (additional)
S J Hardy (additional)
J Holmgren (additional)
L Randall (additional)

References 28 Referenced 89

Cassel , D. , and T. Pfeuffer . 1978 . Mechanism of cholera toxin action: covalent modification ofguanyl nucleotidebinding protein of adenylate cyclase system . Proc. Natl. Acad. Sci. 75 : 2669 - 2673 . (10.1073/pnas.75.6.2669) / Proc. Natl. Acad. Sci. / Mechanism of cholera toxin action: covalent modification ofguanyl nucleotidebinding protein of adenylate cyclase system by Cassel D. (1978)
Clements , J. D. , and R. A. Finkelstein . 1979 . Isolation and characterization of homogenous heat-labile enterotoxins with high specific activity from Escherichia coli cultures . Infect. Immun. 24 : 760 - 769 . (10.1128/IAI.24.3.760-769.1979) / Infect. Immun. / Isolation and characterization of homogenous heat-labile enterotoxins with high specific activity from Escherichia coli cultures by Clements J. D. (1979)
Dallas , W. S. , and S. Falkow . 1979 . The molecular nature of heat-labile enterotoxin (LT) of Escherichia coli . Nature (London) 279 : 406 - 407 . (10.1038/277406a0) / Nature (London) / The molecular nature of heat-labile enterotoxin (LT) of Escherichia coli by Dallas W. S. (1979)
Dallas , W. S. , and S. Falkow . 1980 . Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin . Nature (London) 288 : 499 - 501 . (10.1038/288499a0) / Nature (London) / Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin by Dallas W. S. (1980)
Date , T. , C. Zwizinski , S. Ludmerer , and W. Wickner . 1980 . Mechanisms of membrane assembly: effect of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein . Proc. Natl. Acad. Sci. 77 : 827 - 831 . (10.1073/pnas.77.2.827) / Proc. Natl. Acad. Sci. / Mechanisms of membrane assembly: effect of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein by Date T. (1980)
Dougan , G. , ML Saul , A. Twigg , R. Gill , and D. Sherratt . 1979 . Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tnl and Tn 3 . J. Bacteriol. 138 : 48 - 54 . (10.1128/JB.138.1.48-54.1979) / J. Bacteriol. / Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tnl and Tn 3 by Dougan G. (1979)
Elder , J. HE , R. A. Pickett , J. Hampton , and R. A. Lerner . 1977 . Radioiodination of proteins in single polyacrylamide gel slices . J. Biol. Chem. 252 : 6510 - 6516 . / J. Biol. Chem. / Radioiodination of proteins in single polyacrylamide gel slices by Elder J. HE (1977)
Frazer , A. C. , and R. Curtiss m. 1978 . Production, properties, and utility of bacterial minicells . Curr. Top. Microbiol. Immunol. 69 : 1 - 84 . / Curr. Top. Microbiol. Immunol. / Production, properties, and utility of bacterial minicells by Frazer A. C. (1978)
Gyles , C. , ML So, and S. Falkow . 1974 . The enterotoxin plasmids of Eswherichia coli . J. Infect. Dis. 130 : 40 - 49 . (10.1093/infdis/130.1.40) / J. Infect. Dis. / The enterotoxin plasmids of Eswherichia coli by Gyles C. (1974)
Harold , F. M. 1972 . Conservation and transformation of energy by bacterial membranes . Bacteriol. Rev. 36 : 172 - 230 . (10.1128/BR.36.2.172-230.1972) / Bacteriol. Rev. / Conservation and transformation of energy by bacterial membranes by Harold F. M. (1972)
Hazelbauer , G. L , and S. Harayama . 1979 . Mutants in tran ion of chemotactic signals from two independent receptors of E. coli . Cell 16 : 617 - 625 . (10.1016/0092-8674(79)90035-7) / Cell / Mutants in tran ion of chemotactic signals from two independent receptors of E. coli by Hazelbauer G. L (1979)
Heppel L. A. 1971. The concept of periplasmic enzymes p. 223-247. In L. I. Rothfield (ed.) Structure and func-J. BACTERIOL. (10.1016/B978-0-12-598650-2.50010-7)
tion of biological membranes. Academic Press Inc.
New York.
Hindennach , I. , and U. Henning . 1975 . The major proteins of Escherichia coli outer cell membrane envelope . Eur. J. Biochem. 59 : 207 - 213 . (10.1111/j.1432-1033.1975.tb02443.x) / Eur. J. Biochem. / The major proteins of Escherichia coli outer cell membrane envelope by Hindennach I. (1975)
Holmgren J. and L Lennroth 1980. Structure and function of enterotoxins and their receptors p. 88-103. In 0. Ouchterlony and J. Holmgren (ed.) Cholera and related diarrheas 43rd Nobel Symposium Stockholm 1978. S. Karger AG Basel. (10.1159/000403252)
Inouye , M. , and S. Halegoua 1980 . Secretion and membrane localization of proteins in Escherichia coli . Crit. Rev. Biochem. 7 : 339 - 371 . (10.3109/10409238009105465) / Crit. Rev. Biochem. / Secretion and membrane localization of proteins in Escherichia coli by Inouye M. (1980)
Kunkel , S. , and D. C. Robertson . 1979 . Purification and chemical characterization of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli . Infect. Immun. 25 : 586 - 596 . (10.1128/IAI.25.2.586-596.1979) / Infect. Immun. / Purification and chemical characterization of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli by Kunkel S. (1979)
Lai , C. Y. 1977 . Determination of the primary structure of cholera toxin B subunit . J. Biol. Chem. 252 : 7249 - 7256 . (10.1016/S0021-9258(19)66962-0) / J. Biol. Chem. / Determination of the primary structure of cholera toxin B subunit by Lai C. Y. (1977)
Lai , C. Y. , F. Cancedda , and D. Chang . 1979 . Primary structure of cholera toxin subunit A . FEBS Lett. 100 : 85 - 89 . (10.1016/0014-5793(79)81136-9) / FEBS Lett. / Primary structure of cholera toxin subunit A by Lai C. Y. (1979)
Larson , S. H. , J. Adler , J. J. Gargus , and R. W. Hogg . 1974 . Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria . Proc. Natl. Acad. Sci. 71 : 1239 - 1243 . (10.1073/pnas.71.4.1239) / Proc. Natl. Acad. Sci. / Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria by Larson S. H. (1974)
Miller J. H. 1972. Experiments in molecular genetics p. 431. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Randall , L. L , L. -G. Josefson , and S. J. S. Hardy . 1978 . Processing in vitro of precursor periplasmic proteins from Escherichia coli . Eur. J. Biochem. 92 : 411 - 415 . (10.1111/j.1432-1033.1978.tb12761.x) / Eur. J. Biochem. / Processing in vitro of precursor periplasmic proteins from Escherichia coli by Randall L. L (1978)
So , M. , W. S. Dallas , and S. Falkow . 1978 . Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin . Infect. Immun. 21 : 405 - 411 . (10.1128/IAI.21.2.405-411.1978) / Infect. Immun. / Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin by So M. (1978)
Svennerholm A.-M. and J. Holmgren. 1978. Identifi
cation of Escherichia coli heat labile enterotoxin by
means of ganglioside immunosorbent assay (GMI
ELISA) procedure. Curr. Microbiol. 1:19-27.

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 12:20 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:39 p.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 6:26 p.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Palva_1981, title={Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli}, volume={146}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.146.1.325-330.1981}, DOI={10.1128/jb.146.1.325-330.1981}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Palva, E T and Hirst, T R and Hardy, S J and Holmgren, J and Randall, L}, year={1981}, month=apr, pages={325–330} }

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