Effects of furazlocillin, a beta-lactam antibiotic which binds selectively to penicillin-binding protein 3, on Escherichia coli mutants deficient in other penicillin-binding proteins
10.1128/jb.145.1.632-637.1981
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Furazlocillin binds selectively to penicillin-binding protein 3 (PBP-3), prevents septation of Escherichia coli, and allows the cells to form long filaments without lysis. The effect of furazlocillin on the morphology, autolysis, and murein synthesis of E. coli mutants deficient in either PBP-1A, PBP-1Bs, or PBP-2 was studied. The results reveal that PBP-1A and PBP-1Bs functions are not equivalent since furazlocillin affects the morphology, autolysis, and murein synthesis of PBP1A- mutants quite differently from that of PBP-1Bs mutants. Different "PBP-2-" mutants were found to respond to furazlocillin in dramatically different ways: strain LS-1 cells formed elongated rods with a central bulge which eventually lysed, whereas SP6 cells formed stable "barbells" in which the two daughter cells were well separated but remained connected by a thick central region.

Bibliography

Schmidt, L. S., Botta, G., & Park, J. T. (1981). Effects of furazlocillin, a beta-lactam antibiotic which binds selectively to penicillin-binding protein 3, on Escherichia coli mutants deficient in other penicillin-binding proteins. Journal of Bacteriology, 145(1), 632–637.

Authors 3
  1. L S Schmidt (first)
  2. G Botta (additional)
  3. J T Park (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 11:39 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:22 p.m.)
Indexed 1 month, 2 weeks ago (July 14, 2025, 11:38 p.m.)
Issued 44 years, 7 months ago (Jan. 1, 1981)
Published 44 years, 7 months ago (Jan. 1, 1981)
Published Print 44 years, 7 months ago (Jan. 1, 1981)
Funders 0

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@article{Schmidt_1981, title={Effects of furazlocillin, a beta-lactam antibiotic which binds selectively to penicillin-binding protein 3, on Escherichia coli mutants deficient in other penicillin-binding proteins}, volume={145}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.145.1.632-637.1981}, DOI={10.1128/jb.145.1.632-637.1981}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Schmidt, L S and Botta, G and Park, J T}, year={1981}, month=jan, pages={632–637} }