Positive selection for loss of tetracycline resistance
10.1128/jb.143.2.926-933.1980
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A simple technique has been devised that allows direct plate selection of tetracycline-sensitive clones from a predominantly tetracycline-resistant population. The technique is especially useful in genetic methodologies based on the use of tetracycline resistance transposons, such as Tn10. Potential uses of the method include selection of deletion mutants, fine-structure mapping, generalized mapping, construction of multiply marked strains, elimination of tetracycline resistance transposons and plasmids and cloning. The technique is based on our finding that tetracycline-resistant cells are hypersensitive to lipophilic chelating agents, such as fusaric acid. This finding supports the contention that certain metal ions critically facilitate tetracycline uptake and leads us to suggest possible molecular mechanisms for tetracycline resistance.

Bibliography

Bochner, B. R., Huang, H. C., Schieven, G. L., & Ames, B. N. (1980). Positive selection for loss of tetracycline resistance. Journal of Bacteriology, 143(2), 926–933.

Authors 4
  1. B R Bochner (first)
  2. H C Huang (additional)
  3. G L Schieven (additional)
  4. B N Ames (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 12:06 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:21 p.m.)
Indexed 19 hours, 33 minutes ago (Sept. 4, 2025, 10:22 a.m.)
Issued 45 years, 1 month ago (Aug. 1, 1980)
Published 45 years, 1 month ago (Aug. 1, 1980)
Published Print 45 years, 1 month ago (Aug. 1, 1980)
Funders 0

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@article{Bochner_1980, title={Positive selection for loss of tetracycline resistance}, volume={143}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.143.2.926-933.1980}, DOI={10.1128/jb.143.2.926-933.1980}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bochner, B R and Huang, H C and Schieven, G L and Ames, B N}, year={1980}, month=aug, pages={926–933} }