Positions of early nonsense and deletion mutations in lacZ
10.1128/jb.142.2.732-734.1980
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The positions of three Escherichia coli lacZ operator-proximal nonsense mutations and one deletion mutation have been determined. The nonsense mutations were suppressed with supF, resulting in the production of active beta-galactosidase by each strain. Amino acid sequencing identified the positions of the tyrosine residues inserted by supF, and thereby established that nonsense mutations lacZ2, lacZ2246, and lacZU131 are at sites corresponding to amino acids 23, 36, and 41 of beta-galactosidase, respectively. The deletion mutant, lacZM112, produced a dimeric beta-galactosidase protein missing amino acid residues 23 through 31 of the native enzyme.

Bibliography

Welply, J. K., Fowler, A. V., Beckwith, J. R., & Zabin, I. (1980). Positions of early nonsense and deletion mutations in lacZ. Journal of Bacteriology, 142(2), 732–734.

Authors 4
  1. J K Welply (first)
  2. A V Fowler (additional)
  3. J R Beckwith (additional)
  4. I Zabin (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 12:03 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:37 p.m.)
Indexed 1 year, 6 months ago (Feb. 17, 2024, 10:03 a.m.)
Issued 45 years, 3 months ago (May 1, 1980)
Published 45 years, 3 months ago (May 1, 1980)
Published Print 45 years, 3 months ago (May 1, 1980)
Funders 0

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@article{Welply_1980, title={Positions of early nonsense and deletion mutations in lacZ}, volume={142}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.142.2.732-734.1980}, DOI={10.1128/jb.142.2.732-734.1980}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Welply, J K and Fowler, A V and Beckwith, J R and Zabin, I}, year={1980}, month=may, pages={732–734} }