Precursor in cotranslational secretion of diphtheria toxin
10.1128/jb.141.1.184-189.1980
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on these polysomes were completed in vitro, in the absence of membrane they were found to include not only diphtheria toxin of a molecular weight of 62,000, but also a larger precursor of a molecular weight of 68,000. The precursor was identified by several properties: immune precipitation; conversion into toxin fragments A and B; adenosine diphosphate ribosyl-transferase activity after activation with trypsin; and cleavage to 62,000 daltons by membrane enzymes. The precursor yields an N-terminal A fragment with a broadened molecular weight distribution, compared with that from authentic toxin, thus supporting the expectation that the extra segment of the precursor is N-terminal.

Bibliography

Smith, W. P., Tai, P. C., Murphy, J. R., & Davis, B. D. (1980). Precursor in cotranslational secretion of diphtheria toxin. Journal of Bacteriology, 141(1), 184–189.

Authors 4
  1. W P Smith (first)
  2. P C Tai (additional)
  3. J R Murphy (additional)
  4. B D Davis (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 11:58 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:36 p.m.)
Indexed 1 year, 3 months ago (May 30, 2024, 2:25 a.m.)
Issued 45 years, 8 months ago (Jan. 1, 1980)
Published 45 years, 8 months ago (Jan. 1, 1980)
Published Print 45 years, 8 months ago (Jan. 1, 1980)
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@article{Smith_1980, title={Precursor in cotranslational secretion of diphtheria toxin}, volume={141}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.141.1.184-189.1980}, DOI={10.1128/jb.141.1.184-189.1980}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Smith, W P and Tai, P C and Murphy, J R and Davis, B D}, year={1980}, month=jan, pages={184–189} }