DOI: 10.1128/jb.139.2.591-596.1979. Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens

Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens

10.1128/jb.139.2.591-596.1979

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The host range of Agrobacterium tumefaciens 1D1109, known to induce crown gall only on grapevine (Vitis spp.), was extended to include many plant species by transferring a tumor-inducing plasmid (pTi) from strain 1D1, a broad-host-range pathogen. The pTi plasmid was mobilized by the conjugative plasmid pRK2, which was inserted into 1D1 by mating with Escherichia coli J53(pRK2). The resulting transconjugants were screened for their ability to induce crown gall tumors on hosts other than grapevine by inoculation into sunflower. Transconjugants that were virulent on sunflower were then tested on 36 different host plants and compared with host-limited strain 1D1109 and the donor strain. Two transconjugants induced tumors on the same 28 plant species as those of the original plasmid donor 1D1(pRK2) (pTi). These results show that pRK2 promoted transfer of the pTi plasmid and suggest that the pTi plasmid rather than the A. tumefaciens chromosome determined the host range of the pathogen. Insertion of pRK2 alone did not extend the host range of strain 1D1109. Insertion of pS-a into A. tumefaciens 1D1 by mating with E. coli J53-1 (pS-a) resulted in the concomitant loss of pTi and virulence. There appears to be incompatibility between pTi and pS-a.

Bibliography

Loper, J. E., & Kado, C. I. (1979). Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens. Journal of Bacteriology, 139(2), 591â596.

Authors 2

J E Loper (first)
C I Kado (additional)

References 13 Referenced 87

Bernaerts , M. J. , and J. DeLey . 1963 . A biochemical test for crown gall bacteria . Nature (London) 197 : 406 - 407 . (10.1038/197406b0) / Nature (London) / A biochemical test for crown gall bacteria by Bernaerts M. J. (1963)
Bukhari A. I. J. A. Shapiro and S. L. Adhya (ed.). 1977. DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Chilton , M. - D. , S. K. Farrand , R. Levin , and E. W. Nester . 1976 . RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence . Genetics 83 : 609 - 618 . (10.1093/genetics/83.4.609) / Genetics / RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence by Chilton M. (1976)
Datta N. 1975. Epidemiology and classification of plasmids p. 9-15. In D. Schlessinger (ed.) Microbiology-1974. American Society for Microbiology Washington D.C.
DeCleene , M. , and J. DeLey . 1976 . The host range of crown gall . Bot. Rev. 42 : 389 - 466 . (10.1007/BF02860827) / Bot. Rev. / The host range of crown gall by DeCleene M. (1976)
Hooykaas , P. J. J. , P. M. Klapwijk , M. P. Nuti , R. A. Schilperoort , and R. Rersch . 1977 . Transfer of the Agrobacterium tumefaciens TI plasmid to avirulent Agrobacteria and to Rhizobium ex planta . J. Gen. Microbiol. 98 : 477 - 484 . (10.1099/00221287-98-2-477) / J. Gen. Microbiol. / Transfer of the Agrobacterium tumefaciens TI plasmid to avirulent Agrobacteria and to Rhizobium ex planta by Hooykaas P. J. J. (1977)
Kado , C. I. , M. G. Heskett , and R. A. Langley . 1972 . Studies on Agrobacterium tumefaciens: characterization of strains ID135 and B6, and analysis of the bacterial chromosome, transfer RNA and ribosomes for tumor-inducing ability . Physiol. Plant Pathol. 2 : 47 - 57 . (10.1016/S0048-4059(72)80002-X) / Physiol. Plant Pathol. / Studies on Agrobacterium tumefaciens: characterization of strains ID135 and B6, and analysis of the bacterial chromosome, transfer RNA and ribosomes for tumor-inducing ability by Kado C. I. (1972)
Langley , R. A. , and C. I. Kado . 1972 . Studies on Agrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine and relationships of A. tumefaciens to crown-gall tumor induction . Mutat. Res. 14 : 277 - 286 . (10.1016/0027-5107(72)90014-0) / Mutat. Res. / Studies on Agrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine and relationships of A. tumefaciens to crown-gall tumor induction by Langley R. A. (1972)
McDonell , M. W. , M. W. Simon , and F. W. Studier . 1977 . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels . J. Mol. Biol. 110 : 119 - 146 . (10.1016/S0022-2836(77)80102-2) / J. Mol. Biol. / Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels by McDonell M. W. (1977)
Olsen , R. H. , J. -S. Siak , and R. H. Gray . 1974 . Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage . J. Virol. 14 : 689 - 699 . (10.1128/JVI.14.3.689-699.1974) / J. Virol. / Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage by Olsen R. H. (1974)
Olsen , R. H. , and D. D. Thomas . 1973 . Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid . J. Virol. 12 : 1560 - 1567 . (10.1128/JVI.12.6.1560-1567.1973) / J. Virol. / Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid by Olsen R. H. (1973)
Sonoki S. C. R. Ireland J. E. Loper M. Baraka and C. I. Kado. 1978. New genetic determinants of the virulence plasmid of Agrobacterium tumefaciens p. 133-142. In Station de Pathologie Vegetale et Phytobacteriologie (ed.) Proceedings of the Fourth International Conference on Plant Pathogenic Bacteria Anger France 1978. I.N.R.A. Anger.
Van Larebeke , N. , C. Geneteilo , J. P. Hernalsteens , A. Depicker , I. Zaenen , E. Messens , M. Van Montagu , and J. Schell . 1977 . Transfer of Ti-plasmids between Agrobacterium strains by mobilization with the conjugative plasmid RP4 . Mol. Gen. Genet. 152 : 119 - 124 . (10.1007/BF00268807) / Mol. Gen. Genet. / Transfer of Ti-plasmids between Agrobacterium strains by mobilization with the conjugative plasmid RP4 by Van Larebeke N. (1977)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11:35 a.m.)
Deposited	4 years, 3 months ago (June 4, 2021, 1:19 a.m.)
Indexed	1 year, 7 months ago (Jan. 25, 2024, 12:06 p.m.)
Issued	46 years, 1 month ago (Aug. 1, 1979)
Published	46 years, 1 month ago (Aug. 1, 1979)
Published Print	46 years, 1 month ago (Aug. 1, 1979)

Funders 0

None

BibTeX

@article{Loper_1979, title={Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens}, volume={139}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.139.2.591-596.1979}, DOI={10.1128/jb.139.2.591-596.1979}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Loper, J E and Kado, C I}, year={1979}, month=aug, pages={591–596} }

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