DOI: 10.1128/jb.136.1.304-311.1978. Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis

Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis

10.1128/jb.136.1.304-311.1978

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Eleven succinic dehydrogenase (SDH) mutants in Bacillus subtilis were analyzed by crossed immunoelectrophoresis with antiserum prepared against wild-type B. subtilis cytoplasmic membrane. A precipitate which stained for SDH was found in Triton X-100-solubilized wild-type membranes and in membranes from two of the SDH mutants. The remaining nine mutants did not show an SDH-staining precipitate. The respective mutations in these nine mutants all map in one locus, citF (Ohné et al., J. Bacteriol. 115:738-745, 1973). An SDH-specific antiserum was prepared by immunizing rabbits with the SDH precipitate obtained in crossed immunoelectrophoresis with solubilized wild-type membrane. Using this antiserum, it was shown that all of the nine citF mutants lack an SDH-specific antigen in the membrane but five of the citF mutants have a soluble SDH-specific antigen. No major differences were found in sodium dodecyl sulfatepolyacrylamide gels of membrane proteins from wild-type B. subtilis and from SDH mutants. A model for the organization of SDH in B. subtilis is proposed.

Bibliography

Rutberg, B., Hederstedt, L., Holmgren, E., & Rutberg, L. (1978). Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis. Journal of Bacteriology, 136(1), 304â311.

Authors 4

B Rutberg (first)
L Hederstedt (additional)
E Holmgren (additional)
L Rutberg (additional)

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 11:26 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:31 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:56 p.m.)
Issued	46 years, 11 months ago (Oct. 1, 1978)
Published	46 years, 11 months ago (Oct. 1, 1978)
Published Print	46 years, 11 months ago (Oct. 1, 1978)

Funders 0

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BibTeX

@article{Rutberg_1978, title={Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis}, volume={136}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.136.1.304-311.1978}, DOI={10.1128/jb.136.1.304-311.1978}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rutberg, B and Hederstedt, L and Holmgren, E and Rutberg, L}, year={1978}, month=oct, pages={304–311} }

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