DOI: 10.1128/jb.133.3.1457-1466.1978. Translation of the leader region of the Escherichia coli tryptophan operon

Translation of the leader region of the Escherichia coli tryptophan operon

10.1128/jb.133.3.1457-1466.1978

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

When the trp operon of Escherichia coli contains either of two deletions that fuse the initial portion of the leader region to the distal segments of the trpE gene, novel fusion polypeptides are produced. The new polypeptides are synthesized efficiently both in vivo and in vitro, and their synthesis is subject to repression by trp repressor. Fingerprint analyses of tryptic and chymotryptic digests of the new polypeptides show that both contain trpE polypeptide sequences and, despite their different sizes, share the same N-terminal sequence. Our results suggest that synthesis of the new polypeptides is initiated at the AUG-centered ribosome-binding site in the leader region and proceeds in phase to the region coding for the C-terminal end of the trpE polypeptide.

Bibliography

Miozzari, G. F., & Yanofsky, C. (1978). Translation of the leader region of the Escherichia coli tryptophan operon. Journal of Bacteriology, 133(3), 1457â1466.

Authors 2

G F Miozzari (first)
C Yanofsky (additional)

References 26 Referenced 103

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 11:22 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:28 p.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 6:25 p.m.)
Issued	47 years, 6 months ago (March 1, 1978)
Published	47 years, 6 months ago (March 1, 1978)
Published Print	47 years, 6 months ago (March 1, 1978)

Funders 0

None

BibTeX

@article{Miozzari_1978, title={Translation of the leader region of the Escherichia coli tryptophan operon}, volume={133}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.133.3.1457-1466.1978}, DOI={10.1128/jb.133.3.1457-1466.1978}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Miozzari, G F and Yanofsky, C}, year={1978}, month=mar, pages={1457–1466} }

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