DOI: 10.1128/jb.132.3.996-1002.1977. Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells

Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells

10.1128/jb.132.3.996-1002.1977

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Hybrid Escherichia coli ColE1 plasmids carrying the genes for motility (mot) and chemotaxis (che) were transferred to a minicell-producing strain. The mot and che genes on the hybrid plasmid directed protein synthesis in minicells. Polypeptides synthesized in minicells were identical to the products of the motA, motB, cheA, cheW, cheM, cheX, cheB, cheY, and cheZ genes previously identified by using hybrid lambda and ultraviolet-irradiated host cells (Silverman and Simon, J. Bacteriol. 130:1317-1325, 1977), thus confirming these gene product assignments. The products of some che genes (cheA and cheM) appeared as more than one band on polyacrylamide gel electrophoresis, but analysis of partial peptide digests of these polypeptides suggested that the multiple forms were coded for by a single gene. Measurement of the physical length of the hybrid plasmids allowed an estimate of the amount of coding capacity of the cloned deoxyribonucleic acid, which was devoted to the synthesis of the mot and che gene products. These estimates were also consistent with the hypothesis that the multiple polypeptides corresponding to cheA and cheM were the products of single genes.

Bibliography

Matsumura, P., Silverman, M., & Simon, M. (1977). Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells. Journal of Bacteriology, 132(3), 996â1002.

Authors 3

P Matsumura (first)
M Silverman (additional)
M Simon (additional)

References 14 Referenced 45

Adler J. 19S75. Chemiiotaxis in bacteria. Aninu. Rev. Biochem. 44:34 1-356. (10.1146/annurev.bi.44.070175.002013)
Clarke , L. , and J. Carbon . 1976 . A colony band containing synthetic ColEl hybrid plasmids representative of the entire Escherichia coli genome . Cell 9 : 91 - 99 . (10.1016/0092-8674(76)90055-6) / Cell / A colony band containing synthetic ColEl hybrid plasmids representative of the entire Escherichia coli genome by Clarke L. (1976)
:3. Cleveland , D. W. , S. G. Fischer , M. W. Kirschner , and U. K. Laemmli . 1977 . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by vel electroohoresis . J. Biol. Chem. 251 : 1102 - 1107 . (10.1016/S0021-9258(19)75212-0) / J. Biol. Chem. / Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by vel electroohoresis by Cleveland D. W. (1977)
Laskey R. A. and A. D. Mills. 1975. Quantitative film detection of 'H and '4C in polyacrylamide gels by fluorography. Eur. J1. Biochem. 56::335-341. (10.1111/j.1432-1033.1975.tb02238.x)
Macnab , R. M. , and D. E. Koshland . 1972 . The gradientsensing mechanism in bacterial chemotaxis . P'roc. Natl. Acad. Sci. U.S.A. 69 : 2501 - 2512 . / P'roc. Natl. Acad. Sci. U.S.A. / The gradientsensing mechanism in bacterial chemotaxis by Macnab R. M. (1972)
Matsumura P. M. Silverman and M. Simon. 1977. Cloning aind expression (of the flagellar hook gene bv hybrid plasmids in minicells. Nature (London) 265:758-76W). (10.1038/265758a0)
O'Farrell , P. H. 1975 . High-resolution two-dimensional electrophoresis of proteins. ,J . Biol. Chem. 250 : 400 - 4X7 -4021. (10.1016/S0021-9258(19)41496-8) / Biol. Chem. / High-resolution two-dimensional electrophoresis of proteins. ,J by O'Farrell P. H. (1975)
Silverman , M. , P. Matsumura , R. Draper , S. Edwards , and M. Simon . 1976 . Expression of flagellar genes carried by bacteriophage lambda . Nature (London) 261 : 248 - 250 . (10.1038/261248a0) / Nature (London) / Expression of flagellar genes carried by bacteriophage lambda by Silverman M. (1976)
Silverman , M. , P. Matsumura , M. Hilmen , and M. Simon . 1977 . Characterization of lambda Escherichia coli hybrids carrying chemotaxis genes. .J . Bacteriol. 130 : 877 - 887 . (10.1128/JB.130.2.877-887.1977) / Bacteriol. / Characterization of lambda Escherichia coli hybrids carrying chemotaxis genes. .J by Silverman M. (1977)
Silverman , M. , P. Matsumura , and M. Simon . 1976 . The identification of the mot gene product with Escherichia coli-lambda hybrids . Proc. Natl. Acad. Sci. U.S.A. 73 : 3126 - 3130 . (10.1073/pnas.73.9.3126) / Proc. Natl. Acad. Sci. U.S.A. / The identification of the mot gene product with Escherichia coli-lambda hybrids by Silverman M. (1976)
. Silverman , M. and M. Simon . 1976 . Operon controlling motility and chemotaxis in E. coli . Nature (London) 164 : 577 - 558 (0. (10.1038/264577a0) / Nature (London) / Operon controlling motility and chemotaxis in E. coli by Silverman M. (1976)
Silverman , M. , and M. Simon . 1977 . Identification of polINpeptides necessary for chemotaxis in Escherichia coli . J. Bacteriol. 130 :131 i7 - i1 :325. (10.1128/JB.130.3.1317-1325.1977) / J. Bacteriol. / Identification of polINpeptides necessary for chemotaxis in Escherichia coli by Silverman M. (1977)
Spudich , J. A. , V. Korn , and C. Yanofsky . 1970 . On the production of deletions in the chromosome of Escherichia coli. .1 . Mol. Biol. 53 : 49 - 67 . (10.1016/0022-2836(70)90045-8) / Mol. Biol. / On the production of deletions in the chromosome of Escherichia coli. .1 by Spudich J. A. (1970)
Thomas , M. , and W. Davis . 1975 . Studies on the cleavage of bacteriophage lambda DNA with EcoRl restriction endonuclease . . J. Mol. Biol. 91 : 315 - 328 . (10.1016/0022-2836(75)90383-6) / J. Mol. Biol. / Studies on the cleavage of bacteriophage lambda DNA with EcoRl restriction endonuclease by Thomas M. (1975)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 11:19 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:27 p.m.)
Indexed	1 year, 2 months ago (June 5, 2024, 12:21 p.m.)
Issued	47 years, 9 months ago (Dec. 1, 1977)
Published	47 years, 9 months ago (Dec. 1, 1977)
Published Print	47 years, 9 months ago (Dec. 1, 1977)

Funders 0

None

BibTeX

@article{Matsumura_1977, title={Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells}, volume={132}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.132.3.996-1002.1977}, DOI={10.1128/jb.132.3.996-1002.1977}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Matsumura, P and Silverman, M and Simon, M}, year={1977}, month=dec, pages={996–1002} }

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