DOI: 10.1128/jb.132.3.1027-1030.1977. Reconstitution and purification of flagellar filaments from Caulobacter crescentus

Reconstitution and purification of flagellar filaments from Caulobacter crescentus

10.1128/jb.132.3.1027-1030.1977

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Filaments from isolated flagella of Caulobacter crescentus have been purified by successive dissociation and reconstitution. After the second and third reconstitutions from subunits in 0.8 M sodium citrate, filament preparations contained only two proteins, flagellin A (26,000 daltons) and flagellin B (28,000 daltons). There was some enrichment for flagellin A during reconstitution by this procedure, since isolated flagella contained flagellin A and flagellin B in a ratio of approximately 3.8:1 and filaments after the third reconstitution contained the two proteins in a ratio of 5.0:1.

Bibliography

Sheffery, M., & Newton, A. (1977). Reconstitution and purification of flagellar filaments from Caulobacter crescentus. Journal of Bacteriology, 132(3), 1027â1030.

Authors 2

M Sheffery (first)
A Newton (additional)

References 19 Referenced 27

Abram , D. , and H. Koffler . 1964 . In vitro formation of flagella-like filaments and other structures from flagellin . J. Mol. Biol. 9 : 169 - 185 . (10.1016/S0022-2836(64)80098-X) / J. Mol. Biol. / In vitro formation of flagella-like filaments and other structures from flagellin by Abram D. (1964)
Depamphilis , M. L. , and J. Adler . 1971 . Purification of intact flagella from Escherichia coli and Bacilis subti/is . J. Bacteriol. 105 : 376 - 383 . (10.1128/JB.105.1.376-383.1971) / J. Bacteriol. / Purification of intact flagella from Escherichia coli and Bacilis subti/is by Depamphilis M. L. (1971)
lino, T. 1969 . Genetics and chemistry of bacterial flagella . Bacteriol. Rev. 33 : 454 - 475 . (10.1128/br.33.4.454-475.1969) / Bacteriol. Rev. / Genetics and chemistry of bacterial flagella (1969)
lino, T. 1974 . Assembly of Salmonella flagellin in u!itro and in vito . J. Supramol. Biol. 2 : 372 - 384 . (10.1002/jss.400020226) / J. Supramol. Biol. / Assembly of Salmonella flagellin in u!itro and in vito (1974)
Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T . Nature (London) 227 : 6880 - 6685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T by Laemmli U. K. (1970)
Lagenaur , C. , and N. Agabian . 1976 . Physical characterization of Caulobacter crescentus flagella . J. Bacteriol. 128 : 435 - 444 . (10.1128/JB.128.1.435-444.1976) / J. Bacteriol. / Physical characterization of Caulobacter crescentus flagella by Lagenaur C. (1976)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Osley , M. A. , and A. Newton . 1977 . Mutational analysis of developmental control in Caulobacter crescentus . Proc. Natl. Acad. Sci. U.S.A. 74 : 124 - 128 . (10.1073/pnas.74.1.124) / Proc. Natl. Acad. Sci. U.S.A. / Mutational analysis of developmental control in Caulobacter crescentus by Osley M. A. (1977)
Osley , M. A. , M. Sheffery , and A. Newton . 1977 . Regulation of flagellin synthesis in the cell cycle of Caulobacter: dependence on DNA replication . Cell 12 : 393 - 400 . (10.1016/0092-8674(77)90115-5) / Cell / Regulation of flagellin synthesis in the cell cycle of Caulobacter: dependence on DNA replication by Osley M. A. (1977)
Poindexter , J. S. 1964 . Biological properties and classification of the Caulobacter group . Bacteriol. Rev. 28 : 231 - 295 . (10.1128/BR.28.3.231-295.1964) / Bacteriol. Rev. / Biological properties and classification of the Caulobacter group by Poindexter J. S. (1964)
Schmidt , J. , and R. Y. Stanier . 1966 . IThe development of cellular stalks in bacteria . J. Cell Biol. 28 : 423 - 436 . (10.1083/jcb.28.3.423) / J. Cell Biol. / IThe development of cellular stalks in bacteria by Schmidt J. (1966)
Shapiro L. and J. V. Maizel Jr. 197.3. Synthesis and structure of Caulobacter crescentus flagella. J. Bacteriol. 113:478-485. (10.1128/jb.113.1.478-485.1973)
Smith , R. W. , and H. Koffler . 1971 . Bacterial flagella . Adv. Microb. Physiol. 6 : 219 - 338 . (10.1016/S0065-2911(08)60070-3) / Adv. Microb. Physiol. / Bacterial flagella by Smith R. W. (1971)
Wakabayashi , K. , H. Hotani , and S. Asakura . 1969 . Polymerization of Salmonella flagellin in the presence of high concentrations of salts . Biochim. Biophys. Acta 175 : 195 - 120 :3. (10.1016/0005-2795(69)90157-3) / Biochim. Biophys. Acta / Polymerization of Salmonella flagellin in the presence of high concentrations of salts by Wakabayashi K. (1969)
tions. Filaments were reconstituted from the acid
soluble material as described in the text: first recon
stitution (well 4) second reconstitution (well 5) and
third reconstitution (well 6). The gel was stained with
.25T Coomassie brilliant blue.

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 10:59 a.m.)
Deposited	4 years, 2 months ago (June 5, 2021, 6:51 a.m.)
Indexed	1 year, 9 months ago (Nov. 20, 2023, 10:33 p.m.)
Issued	47 years, 8 months ago (Dec. 1, 1977)
Published	47 years, 8 months ago (Dec. 1, 1977)
Published Print	47 years, 8 months ago (Dec. 1, 1977)

Funders 0

None

BibTeX

@article{Sheffery_1977, title={Reconstitution and purification of flagellar filaments from Caulobacter crescentus}, volume={132}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.132.3.1027-1030.1977}, DOI={10.1128/jb.132.3.1027-1030.1977}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sheffery, M and Newton, A}, year={1977}, month=dec, pages={1027–1030} }

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