DOI: 10.1128/jb.130.1.167-172.1977. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications

Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications

10.1128/jb.130.1.167-172.1977

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A method is described for the isolation of Escherichia coli mutants that show increased recombination between a pair of chromosomal duplications. These "hyper-rec" mutants display a variety of secondary phenotypes. I have isolated a large number of hyper-rec mutants and found them useful in screening for mutants that accumulate labeled DNA fragments after short pulses with [3H]thymidine. The mutants so recovered include ones that are defective in deoxyribonucleic acid ligase, deoxyribonucleic acid polymerase I and its associated 5' yields 3' exonuclease, and a group of mutants, dnaS, that accumulate abnormally short Okazaki fragments. Evidence is presented that suggests that the lac-att80 segment of the chromosome cannot be inverted.

Bibliography

Konrad, E. B. (1977). Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. Journal of Bacteriology, 130(1), 167â172.

Authors 1

E B Konrad (first)

References 20 Referenced 154

Bachmann , B. J. , K. B. Low , and C. L. Taylor . 1976 . Recalibrated linkage map of Escherichia coli K-12 . Bacteriol. Rev. 40 : 116 - 167 . (10.1128/br.40.1.116-167.1976) / Bacteriol. Rev. / Recalibrated linkage map of Escherichia coli K-12 by Bachmann B. J. (1976)
Beckwith , J. R. 1964 . A deletion analysis of the lac operator region in Escherichia coli . J. Mol. Biol. 8 : 427 - 430 . (10.1016/S0022-2836(64)80206-0) / J. Mol. Biol. / A deletion analysis of the lac operator region in Escherichia coli by Beckwith J. R. (1964)
De Lucia , P. , and J. Cairns . 1969 . Isolation of an Escherichia coli strain with a mutation affecting DNA polymerase . Nature (London) 224 : 1164 - 1166 . (10.1038/2241164a0) / Nature (London) / Isolation of an Escherichia coli strain with a mutation affecting DNA polymerase by De Lucia P. (1969)
Gottesman , M. M. , M. L. Hicks , and M. Gellert . 1973 . Genetics and function of DNA ligase in Escherichia coli . J. Mol. Biol. 77 : 531 - 547 . (10.1016/0022-2836(73)90221-0) / J. Mol. Biol. / Genetics and function of DNA ligase in Escherichia coli by Gottesman M. M. (1973)
Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Therosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 686 . (10.1101/SQB.1968.033.01.077) / Cold Spring Harbor Symp. Quant. Biol. / Therosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division by Hirota Y. (1968)
Jacob , F. , and J. Monod . 1961 . Genetic regulatory mechanisms in the synthesis of proteins . J. Mol. Biol. 3 : 318 - 356 . (10.1016/S0022-2836(61)80072-7) / J. Mol. Biol. / Genetic regulatory mechanisms in the synthesis of proteins by Jacob F. (1961)
Konrad , E. B. , and I. R. Lehman . 1974 . A conditional lethal mutant ofEscherichia coli K12 defective in the 5' 3' exonuclease associated with DNA polymerase I . Proc. Natl. Acad. Sci. U.S.A. 71 : 2048 - 2051 . (10.1073/pnas.71.5.2048) / Proc. Natl. Acad. Sci. U.S.A. / A conditional lethal mutant ofEscherichia coli K12 defective in the 5' 3' exonuclease associated with DNA polymerase I by Konrad E. B. (1974)
Konrad , E. B. , and I. R. Lehman . 1975 . Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates . Proc. Natl. Acad. Sci. U.S.A. 72 : 2150 - 2154 . (10.1073/pnas.72.6.2150) / Proc. Natl. Acad. Sci. U.S.A. / Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates by Konrad E. B. (1975)
Konrad , E. B. , P. Modrich , and I. R. Lehman . 1973 . Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase . J. Mol. Biol. 77 : 519 - 529 . (10.1016/0022-2836(73)90220-9) / J. Mol. Biol. / Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase by Konrad E. B. (1973)
Kuempel , P. L. , and G. E. Veomett . 1970 . A possible function of DNA polymerase in chromosomal replication . Biochem. Biophys. Res. Commun. 41 : 973 - 980 . (10.1016/0006-291X(70)90180-4) / Biochem. Biophys. Res. Commun. / A possible function of DNA polymerase in chromosomal replication by Kuempel P. L. (1970)
Marinus , M. G. , and N. R. Morris . 1974 . Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12 . J. Mol. Biol. 85 : 309 - 322 . (10.1016/0022-2836(74)90366-0) / J. Mol. Biol. / Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12 by Marinus M. G. (1974)
Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Modrich , P. , and I. R. Lehman . 1970 . Enzymatic joining of polynucleotides . J. Biol. Chem. 245 : 3626 - 3631 . (10.1016/S0021-9258(18)62972-2) / J. Biol. Chem. / Enzymatic joining of polynucleotides by Modrich P. (1970)
Okazaki , R. , M. Arisawa , and A. Sugino . 1971 . Slow joining of newly replicate DNA chains in DNA polymerase I-deficientEscherichia coli mutants . Proc. Natl. Acad. Sci. U.S.A. 66 : 2954 - 2957 . (10.1073/pnas.68.12.2954) / Proc. Natl. Acad. Sci. U.S.A. / Slow joining of newly replicate DNA chains in DNA polymerase I-deficientEscherichia coli mutants by Okazaki R. (1971)
Okazaki R. A. Sugino S. Hirosa T. Okazaki Y. Imae R. Kainuma-Kurodo T. Ogawa M. Arisawa and Y. Kurosawa. 1973. Discontinuous replication of DNA p. 81-104. In R. D. Wells and R. B. Inman (ed.) DNA synthesis in vitro. University Park Press Baltimore. (10.1007/978-94-011-6132-9_8)
Olivera , B. M. , and F. Bonhoeffer . 1974 . Replication of Escherichia coli requires DNA polymerase I . Nature (London) 250 : 513 - 514 . (10.1038/250513a0) / Nature (London) / Replication of Escherichia coli requires DNA polymerase I by Olivera B. M. (1974)
Pauling , G. , and L. Hamm . 1968 . Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli . Proc. Natl. Acad. Sci. U.S.A. 60 : 1495 - 1502 . (10.1073/pnas.60.4.1495) / Proc. Natl. Acad. Sci. U.S.A. / Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli by Pauling G. (1968)
Pauling , G. , and L. Hamm . 1969 . Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication . Proc. Natl. Acad. Sci. U.S.A. 64 : 1195 - 1202 . (10.1073/pnas.64.4.1195) / Proc. Natl. Acad. Sci. U.S.A. / Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication by Pauling G. (1969)
Siegel , E. C. 1973 . Ultraviolet-sensitive mutator strain of Escherichza coli K-12 . J. Bacteriol. 113 : 145 - 160 . (10.1128/jb.113.1.145-160.1973) / J. Bacteriol. / Ultraviolet-sensitive mutator strain of Escherichza coli K-12 by Siegel E. C. (1973)
Signer , E. R. , and J. R. Beckwith . 1966 . Transposition of the lactose region of Escherichia coli. III. The mechanism of attachment of bacteriophage 480 to the bacterial chromosome . J. Mol. Biol. 22 : 33 - 51 . / J. Mol. Biol. / Transposition of the lactose region of Escherichia coli. III. The mechanism of attachment of bacteriophage 480 to the bacterial chromosome by Signer E. R. (1966)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11:14 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:24 p.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 12:26 a.m.)
Issued	48 years, 5 months ago (April 1, 1977)
Published	48 years, 5 months ago (April 1, 1977)
Published Print	48 years, 5 months ago (April 1, 1977)

Funders 0

None

BibTeX

@article{Konrad_1977, title={Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications}, volume={130}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.130.1.167-172.1977}, DOI={10.1128/jb.130.1.167-172.1977}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Konrad, E B}, year={1977}, month=apr, pages={167–172} }

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