DOI: 10.1128/jb.129.2.1001-1009.1977. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes

Biochemical parameters of glutamine synthetase from Klebsiella aerogenes

10.1128/jb.129.2.1001-1009.1977

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The glutamine synthetase (GS) from Klebsiella aerogenes is similar to that from Escherichia coli in several respects: (i) it is repressed by high levels of ammonia in the growth medium; (ii) its biosynthetic activity is greatly reduced by adenylylation; and (iii) adenylylation lowers the pH optimum and alters the response of the enzymes to various inhibitors in the gamma-glutamyl transferase (gammaGT) assay. There are, however, several important differences: (i) the isoactivity point for the adenylylated and non-adenylylated forms in the gammaGT assay occurs at pH 7.55 in K. aerogenes and at pH 7.15 in E. coli; (ii) the non-adenylylated form of the GS from K. aerogenes is stimulated by 60 mM MgCl2 in the gammaGT assay at pH 7.15. A biosynthetic reaction assay that correlates well with number of non-adenylylated enzyme subunits, as determined by the method of Mg2+ inhibition of the gammaGT assay, is described. Finally, we have found that it is necessary to use special methods to harvest growing cells to prevent changes in the adenylylation state of GS from occurring during harvesting.

Bibliography

Bender, R. A., Janssen, K. A., Resnick, A. D., Blumenberg, M., Foor, F., & Magasanik, B. (1977). Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. Journal of Bacteriology, 129(2), 1001â1009.

Authors 6

R A Bender (first)
K A Janssen (additional)
A D Resnick (additional)
M Blumenberg (additional)
F Foor (additional)
B Magasanik (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 11:09 a.m.)
Deposited	4 years, 2 months ago (June 12, 2021, 1:24 p.m.)
Indexed	1 day, 21 hours ago (Sept. 4, 2025, 10:16 a.m.)
Issued	48 years, 7 months ago (Feb. 1, 1977)
Published	48 years, 7 months ago (Feb. 1, 1977)
Published Print	48 years, 7 months ago (Feb. 1, 1977)

Funders 0

None

BibTeX

@article{Bender_1977, title={Biochemical parameters of glutamine synthetase from Klebsiella aerogenes}, volume={129}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.129.2.1001-1009.1977}, DOI={10.1128/jb.129.2.1001-1009.1977}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bender, R A and Janssen, K A and Resnick, A D and Blumenberg, M and Foor, F and Magasanik, B}, year={1977}, month=feb, pages={1001–1009} }

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