Specific deficit in the synthesis of 6-sulfoquinovsyl diglyceride in Chlorella pyrenoidosa
10.1128/jb.129.1.516-524.1977
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

It was found that when Chlorella pyrenoidosa was grown on cysteine as the sole sulfur source, it lost the ability to grow photoautotrophically. When grown in the presence of glucose, cysteine-grown cells displayed a doubling time in the light or dark of 45 h, which is identical to that of cells grown on glucose and SO4 in the dark. This suggests that cells grown on cysteine as sole sulfur source can only grow heterotrophically. In support of this hypothesis, it was found that cysteine-grown cells were defective both in vivo and in vitro in CO2 fixation, although O2 evolution in such cells was normal. Assays of the enzymes of the Calvin cycle indicated that the deficit in CO2 fixation could be ascribed to a lowered phosphoribulokinase activity. A total lipid analysis of Chlorella grown on cysteine revealed that such cells showed a 100-fold deficiency in the purportedly chloroplast-associated 6-sulfoquinovsyl diglyceride. This agrees with earlier reports that cysteine could not serve as a precursor of sulfolipid in Chlorella. No other polar lipid was affected. Large amounts of triglyceride, however, were found in cysteine-grown cells. The biosynthesis of triglyceride provides a means of utilizing reduced nicotinamide adenine dinucleotide reducing equivalents not being used for CO2 fixation.

Bibliography

Sinensky, M. (1977). Specific deficit in the synthesis of 6-sulfoquinovsyl diglyceride in Chlorella pyrenoidosa. Journal of Bacteriology, 129(1), 516–524.

Authors 1
  1. M Sinensky (first)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 11:08 a.m.)
Deposited 4 years ago (July 29, 2021, 1:23 p.m.)
Indexed 2 years, 6 months ago (Jan. 30, 2023, 11:53 a.m.)
Issued 48 years, 7 months ago (Jan. 1, 1977)
Published 48 years, 7 months ago (Jan. 1, 1977)
Published Print 48 years, 7 months ago (Jan. 1, 1977)
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@article{Sinensky_1977, title={Specific deficit in the synthesis of 6-sulfoquinovsyl diglyceride in Chlorella pyrenoidosa}, volume={129}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.129.1.516-524.1977}, DOI={10.1128/jb.129.1.516-524.1977}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sinensky, M}, year={1977}, month=jan, pages={516–524} }