Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants
10.1128/jb.127.3.1070-1079.1976
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Two classes of mutants, con and tolG, that appeared to be very similar in a number of respects have been shown to be identical and cotransducible with pyrD. By diethylaminoethyl-cellulose chromatography of the outer membranes, we have shown that the mutants are missing only protein 3A and retain protein 3B. Using con mutants, we were thus able to identify protein 3B on the pH 7.2 gel system of Maizel where it runs separately from protein 3A if unheated samples are used. tolG mutants were shown to be identical to con mutants in being conjugation defective with most F-like plasmid donors but not with I-like plasmid donors, and in their resistance pattern to bacteriophages and colicins. During the course of this study, it was observed that the bacteriocin produced by Serratia marcescenc JF246 was identical in its activity spectrum to colicin L-398 and is now considered to be a colicin of type L.

Bibliography

Manning, P. A., & Reeves, P. (1976). Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants. Journal of Bacteriology, 127(3), 1070–1079.

Authors 2
  1. P A Manning (first)
  2. P Reeves (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 11:04 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:22 p.m.)
Indexed 1 year, 11 months ago (Sept. 17, 2023, 4:59 a.m.)
Issued 49 years ago (Sept. 1, 1976)
Published 49 years ago (Sept. 1, 1976)
Published Print 49 years ago (Sept. 1, 1976)
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@article{Manning_1976, title={Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants}, volume={127}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.127.3.1070-1079.1976}, DOI={10.1128/jb.127.3.1070-1079.1976}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Manning, P A and Reeves, P}, year={1976}, month=sep, pages={1070–1079} }