DOI: 10.1128/jb.126.1.377-383.1976. Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum

Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum

10.1128/jb.126.1.377-383.1976

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Clostridum thermosaccharolyticum and Clostridium thermohydrosulfuricum possess as outermost cell wall layer a tetragonal or hexagonal ordered array of macromolecules. The subunits of the surface layer can be detached from isolated cell walls with urea (8M) or guanidine-HCl (4 to 5 M). Triton X-100, dithiothreitol, ethylenediaminetetracetate, and KCl (3 M) had no visible effect on the regular arrays. Sodium dodecyl sulfate-polyacrylamide electrophroesis showed that, in both organisms, the surface layer is composed of glycoprotein of molecular weight 140,000. The glycoprotein from both microorganisms has a predominantly acidic amino acid composition and an acidic isoelectric point after isoelectric focusing on polyacrylamide gels. The glycocomponent is composed of glucose, galactose, mannose, and rhamnose.

Bibliography

Sleytr, U. B., & Thorne, K. J. (1976). Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum. Journal of Bacteriology, 126(1), 377â383.

Authors 2

U B Sleytr (first)
K J Thorne (additional)

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 11:02 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:21 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 5:09 a.m.)
Issued	49 years, 4 months ago (April 1, 1976)
Published	49 years, 4 months ago (April 1, 1976)
Published Print	49 years, 4 months ago (April 1, 1976)

Funders 0

None

BibTeX

@article{Sleytr_1976, title={Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum}, volume={126}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.126.1.377-383.1976}, DOI={10.1128/jb.126.1.377-383.1976}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sleytr, U B and Thorne, K J}, year={1976}, month=apr, pages={377–383} }

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