DOI: 10.1128/jb.117.2.444-448.1974. Structural Interactions Between Amino Acid Residues at Positions 22 and 211 in the Tryptophan Synthetase Alpha Chain of <i>Escherichia coli</i>

Structural Interactions Between Amino Acid Residues at Positions 22 and 211 in the Tryptophan Synthetase Alpha Chain of Escherichia coli

10.1128/jb.117.2.444-448.1974

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Construction and characterization of double mutants altered in the structural gene of the tryptophan synthetase alpha chain of Escherichia coli revealed interactions between amino acid residues at positions 22 and 211. These interactions are specific for the particular amino acid residue at position 211. The results indicate also that amino acid residues which appear to be functionally near-equivalent in one configuration may strongly influence the activity of a protein with a subsequent change at another site. Seven independent suppressors of trpA218 (Leu22-Ser211) were isolated. Their properties suggest that all seven may suppress the codon (AGU/C) for Ser211. Six of the seven are co-transducible with glyV , the structural gene for the GGU/C-specific tRNA Gly .

Bibliography

Murgola, E. J., & Yanofsky, C. (1974). Structural Interactions Between Amino Acid Residues at Positions 22 and 211 in the Tryptophan Synthetase Alpha Chain of Escherichia coli. Journal of Bacteriology, 117(2), 444â448.

Authors 2

E. J. Murgola (first)
C. Yanofsky (additional)

References 9 Referenced 51

Allen , M. K. , and C. Yanofsky . 1963 . A biochemical and genetic study of reversion with the A-gene A-protein system of Escherichia c61i tryptophan synthetase . Genetics 48 : 1065 - 1083 . (10.1093/genetics/48.8.1065) / Genetics / A biochemical and genetic study of reversion with the A-gene A-protein system of Escherichia c61i tryptophan synthetase by Allen M. K. (1963)
Carbon , J. , C. Squires , and C. W. Hill . 1970 . Glycine transfer RNA of Escherichia coli. IL Impaired GGArecognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation . J. Mol. Biol. 52 : 571 - 584 . / J. Mol. Biol. / Glycine transfer RNA of Escherichia coli. IL Impaired GGArecognition in strains containing a genetically altered transfer RNA; reversal by a secondary suppressor mutation by Carbon J. (1970)
Hong , J. - S. , and B. N. Ames . 1971 . Localized mutagenesis of any specific small region of the bacterial chromosome . Proc. Nat. Acad. Sci. U.S.A. 68 : 3158 - 3162 . (10.1073/pnas.68.12.3158) / Proc. Nat. Acad. Sci. U.S.A. / Localized mutagenesis of any specific small region of the bacterial chromosome by Hong J. (1971)
Lennox , E. S. 1955 . Transduction of linked genetic characters ofthe host by bacteriophage P1 . Virology 1 : 190 - 206 . (10.1016/0042-6822(55)90016-7) / Virology / Transduction of linked genetic characters ofthe host by bacteriophage P1 by Lennox E. S. (1955)
Squires , C. , and J. Carbon . 1971 . Normal and mutant glycine transfer RNAs . Nature N. Biol. 233 : 274 - 277 . (10.1038/newbio233274a0) / Nature N. Biol. / Normal and mutant glycine transfer RNAs by Squires C. (1971)
Vogel , H. J. , and D. M. Bonner . 1956 . A convenient growth medium for E. coli and some other microorganisms (medium E) . Microbial Genet. Bull. 13 : 43 - 44 . - / Microbial Genet. Bull. / A convenient growth medium for E. coli and some other microorganisms (medium E) by Vogel H. J. (1956)
Yanofsky , C. 1969 . Protein structure and evolution. In M. Marois (ed.), Proc. 2nd Int. Conf. on Theoret. Phys. Biol., p. 191 - 206 . Centre National de la Recherche Scientifique, Paris. / Int. Conf. on Theoret. Phys. Biol., p. / Protein structure and evolution. In M. Marois (ed.), Proc. 2nd by Yanofsky C. (1969)
Yanofsky , C. , and V. Horn . 1972 . Tryptophan synthe. tase a chain positions affected by mutations near the ends of the genetic map of trpA of Escherichia coUl . J. Biol. Chem. 247 : 4494 - 4498 . (10.1016/S0021-9258(19)45013-8) / J. Biol. Chem. / Tryptophan synthe. tase a chain positions affected by mutations near the ends of the genetic map of trpA of Escherichia coUl by Yanofsky C. (1972)
Yanofsky , C. , J. Ito , and V. Hom . 1966 . Amino acid replacements and the genetic code . Cold Sprng Harbor Symp. Quant. Biol. 31 : 151 - 162 . (10.1101/SQB.1966.031.01.023) / Cold Sprng Harbor Symp. Quant. Biol. / Amino acid replacements and the genetic code by Yanofsky C. (1966)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:56 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:11 p.m.)
Indexed	2 months ago (July 2, 2025, 2:48 p.m.)
Issued	51 years, 7 months ago (Feb. 1, 1974)
Published	51 years, 7 months ago (Feb. 1, 1974)
Published Print	51 years, 7 months ago (Feb. 1, 1974)

Funders 0

None

BibTeX

@article{Murgola_1974, title={Structural Interactions Between Amino Acid Residues at Positions 22 and 211 in the Tryptophan Synthetase Alpha Chain of Escherichia coli}, volume={117}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.117.2.444-448.1974}, DOI={10.1128/jb.117.2.444-448.1974}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Murgola, E. J. and Yanofsky, C.}, year={1974}, month=feb, pages={444–448} }

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