DOI: 10.1128/jb.117.1.252-260.1974. Mutants of <i>Saccharomyces cerevisiae</i> That Incorporate Deoxythymidine-5′-Monophosphate Into Deoxyribonucleic Acid In Vivo

Mutants of Saccharomyces cerevisiae That Incorporate Deoxythymidine-5′-Monophosphate Into Deoxyribonucleic Acid In Vivo

10.1128/jb.117.1.252-260.1974

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Spontaneous mutants of Saccharomyces cerevisiae able to incorporate deoxythymidine-5′-monophosphate (dTMP) into deoxyribonucleic acid (DNA) have been selected based on their ability to grow in the presence of aminopterin and sulfanilamide if dTMP is present. Essentially all mutants (called tup ) selected in this way required dTMP for growth in the presence of the two drugs, but none required dTMP in the absence of the drugs. Neither thymine nor thymidine would satisfy this requirement. Equimolar amounts of 32 P- and 3 H-base-labeled dTMP were incorporated by the mutants into alkali-stable, deoxyribonuclease-sensitive material. In the presence of aminopterin and sulfanilamide, this incorporation was sufficient to account for a substantial proportion of the thymine residues in the cellular DNA, whereas in the absence of the drugs only about 40% as much of the thymine residues originated from the medium. Of 29 mutants examined, all were recessive and 17 showed 2:2 segregation in crosses with a wild-type strain. The lesions in these mutants fell into four complementation groups: one ( tup1 ) occurs on chromosome III; another ( tup3 ) is on chromosome II; and a third ( tup4 ) was centromere linked. Strains of the genotype α tup1 mated with lower than normal efficiency with a strains, but with higher than normal efficiency with α strains. Strains of genotype a/α tup1/tup1 failed to sporulate, whereas homozygous diploids for tup2, tup3 , or tup4 sporulated normally, as did a/α tup1 /+ strains.

Bibliography

Wickner, R. B. (1974). Mutants of Saccharomyces cerevisiae That Incorporate Deoxythymidine-5â²-Monophosphate Into Deoxyribonucleic Acid In Vivo. Journal of Bacteriology, 117(1), 252â260.

Authors 1

Reed B. Wickner (first)

References 19 Referenced 102

Brendel , M. , and R. H. Haynes . 1972 . Kinetics and genetic control of the incorporation of thymidine monophosphate in yeast DNA . Mol. Gen. Genet. 117 : 39 - 44 . (10.1007/BF00268835) / Mol. Gen. Genet. / Kinetics and genetic control of the incorporation of thymidine monophosphate in yeast DNA by Brendel M. (1972)
Garrod , L. P. , D. G. James , and A. A. G. Lewis (ed.). 1969 . The synergy of trimethoprim and sulphonamides . Postgrad. Med. J. 45:suppl. / Postgrad. Med. J. 45:suppl. / The synergy of trimethoprim and sulphonamides by Garrod L. P. (1969)
Goldring , E. S. , L. I. Grossman , D. Krupnick , D. R. Cryer , and J. Marmur . 1970 . The petite mutation in yeast: loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide . J. Mol. Biol. 52 : 323 - 335 . (10.1016/0022-2836(70)90033-1) / J. Mol. Biol. / The petite mutation in yeast: loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide by Goldring E. S. (1970)
Grivell , A. R. , and J. F. Jackson . 1968 . Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid . J. Gen. Microbiol. 54 : 307 - 317 . (10.1099/00221287-54-2-307) / J. Gen. Microbiol. / Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid by Grivell A. R. (1968)
Hartwell , L. H. , J. Culotti , and B. Reid . 1970 . Genetic control of the cell-division cycle in yeast. I. Detection of mutants . Proc. Nat. Acad. Sci. U.S.A. 66 : 352 - 359 . (10.1073/pnas.66.2.352) / Proc. Nat. Acad. Sci. U.S.A. / Genetic control of the cell-division cycle in yeast. I. Detection of mutants by Hartwell L. H. (1970)
Hawthorne , D. C. , and R. K. Mortimer . 1960 . Chromosome mapping in Saccharomyces: centromerelinked genes . Genetics 45 : 1085 - 1110 . (10.1093/genetics/45.8.1085) / Genetics / Chromosome mapping in Saccharomyces: centromerelinked genes by Hawthorne D. C. (1960)
Jannsen , S. , E. R. Lochmann , and W. Laskowski . 1968 . DNS-Synthese nach R5ntgenbestrahlung bei homozygoten Hefestimmen Ploidiegrades . Z. Naturforsch. B. 23 : 1500 - 1507 . (10.1515/znb-1968-1113) / Z. Naturforsch. B. / DNS-Synthese nach R5ntgenbestrahlung bei homozygoten Hefestimmen Ploidiegrades by Jannsen S. (1968)
Jannsen , S. , - E. R. Lochmann , and R. Megnet . 1970 . Specific incorporation of exogenous thymidine monophosphate into DNA in Saccharomyces cerevisiae . FEBS Lett. 8 : 113 - 115 . (10.1016/0014-5793(70)80239-3) / FEBS Lett. / Specific incorporation of exogenous thymidine monophosphate into DNA in Saccharomyces cerevisiae by Jannsen S. (1970)
Jannsen , S. , I. Witte , and R. Megnet . 1973 . Mutants for the specific labelling of DNA in Saccharomyces cerevisiae . Biochim. Biophys. Acta 299 : 681 - 685 . (10.1016/0005-2787(73)90243-8) / Biochim. Biophys. Acta / Mutants for the specific labelling of DNA in Saccharomyces cerevisiae by Jannsen S. (1973)
Johnston , J. R. , and R. K. Mortimer . 1959 . Use of snail digestive juice in isolation of yeast spore tetrads . J. Bacteriol. 78 : 292 . (10.1128/JB.78.2.292-292.1959) / J. Bacteriol. / Use of snail digestive juice in isolation of yeast spore tetrads by Johnston J. R. (1959)
Lehman , I. R. , M. J. Bessman , E. S. Simms , and A. Kornberg . 1958 . Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of-an enzyme from Escherichia coli . J. Biol. Chem. 233 : 163 - 170 . / J. Biol. Chem. / Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of-an enzyme from Escherichia coli by Lehman I. R. (1958)
Mortimer , R. K. , and D. C. Hawthorne . 1966 . Genetic mapping in Saccharomyces . Genetics 53 : 165 - 173 . (10.1093/genetics/53.1.165) / Genetics / Genetic mapping in Saccharomyces by Mortimer R. K. (1966)
Nickerson , W. J. , and M. Webb . 1956 . Effect of folic acid analogues on growth and cell division of non-exacting microorganisms . J. Bacteriol. 71 : 129 - 139 . (10.1128/JB.71.2.129-139.1956) / J. Bacteriol. / Effect of folic acid analogues on growth and cell division of non-exacting microorganisms by Nickerson W. J. (1956)
Ogur , M. , S. Minckler , G. Lindegren , and C. C. Lindegren . 1952 . The nucleic acids in a polyploid series of Saccharomyces . Arch. Biochem. Biophys. 40 : 175 - 184 . (10.1016/0003-9861(52)90085-4) / Arch. Biochem. Biophys. / The nucleic acids in a polyploid series of Saccharomyces by Ogur M. (1952)
Okada , T. , K. Kanagisawa , and F. J. Ryan . 1961 . A method for securing thymineless mutants from strains . Z. Vererbungslehre. 92 : 403 - 412 . / Z. Vererbungslehre. / A method for securing thymineless mutants from strains by Okada T. (1961)
Perkins , D. D. 1949 . Biochemical mutants in the smut fungus Ustilago maydis . Genetics 34 : 607 - 626 . (10.1093/genetics/34.5.607) / Genetics / Biochemical mutants in the smut fungus Ustilago maydis by Perkins D. D. (1949)
Stacey , K. A. , and E. Simson . 1965 . Improved method for the isolation of thymine-requiring mutants of Escherichia coli . J. Bacteriol. 90 : 554 - 555 . (10.1128/JB.90.2.554-555.1965) / J. Bacteriol. / Improved method for the isolation of thymine-requiring mutants of Escherichia coli by Stacey K. A. (1965)
Wickerham , L. J. 1946 . A critical evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts . J. Bacteriol. 52 : 293 - 301 . (10.1128/JB.52.3.293-301.1946) / J. Bacteriol. / A critical evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts by Wickerham L. J. (1946)
Young , E. T. , and R. L. Sinsheimer . 1967 . Vegetative bacteriophage A DNA. II. Physical characterization and replication . J. Mol. Biol. 30 : 165 - 200 . (10.1016/0022-2836(67)90251-3) / J. Mol. Biol. / Vegetative bacteriophage A DNA. II. Physical characterization and replication by Young E. T. (1967)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:56 a.m.)
Deposited	4 years ago (July 29, 2021, 1:12 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:19 a.m.)
Issued	51 years, 7 months ago (Jan. 1, 1974)
Published	51 years, 7 months ago (Jan. 1, 1974)
Published Print	51 years, 7 months ago (Jan. 1, 1974)

Funders 0

None

BibTeX

@article{Wickner_1974, title={Mutants of Saccharomyces cerevisiae That Incorporate Deoxythymidine-5′-Monophosphate Into Deoxyribonucleic Acid In Vivo}, volume={117}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.117.1.252-260.1974}, DOI={10.1128/jb.117.1.252-260.1974}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wickner, Reed B.}, year={1974}, month=jan, pages={252–260} }

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