DOI: 10.1128/jb.116.3.1091-1095.1973. Identification of the Amide-Linked Fatty Acids of <i>Acholeplasma axanthum</i> S743 as <scp>d</scp> (–)3-Hydroxyhexadecanoate and Its Homologues

Identification of the Amide-Linked Fatty Acids of Acholeplasma axanthum S743 as d (–)3-Hydroxyhexadecanoate and Its Homologues

10.1128/jb.116.3.1091-1095.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The amide-linked fatty acids of the sphingolipids of Acholeplasma axanthum S743 are predominantly hydroxy acids. These acids were shown by gas-liquid chromatography, mass spectrometry, and polarimetry to be the d (–)3-hydroxy fatty acids. The predominant component of the mixture was 3-hydroxyhexadecanoate (β-hydroxypalmitate, hydroxy [h] 16:0) followed by h 20Δ (Δ = unsaturated), h14:0, h12:0, and h18Δ in decreasing order of concentration. The fatty acid profile indicates that these β-hydroxy acids possibly arise from elongation of the fatty acids supplied in the growth medium.

Bibliography

Mayberry, W. R., Smith, P. F., Langworthy, T. A., & Plackett, P. (1973). Identification of the Amide-Linked Fatty Acids of Acholeplasma axanthum S743 as d (â)3-Hydroxyhexadecanoate and Its Homologues. Journal of Bacteriology, 116(3), 1091â1095.

Authors 4

William R. Mayberry (first)
P. F. Smith (additional)
Thomas A. Langworthy (additional)
Philip Plackett (additional)

References 17 Referenced 28

Carter , H. E. , and R. C. Gaver . 1967 . Improved reagent for trimethylsilylation of sphingolipid bases . J. Lipid Res. 8 : 391 - 395 . (10.1016/S0022-2275(20)39571-7) / J. Lipid Res. / Improved reagent for trimethylsilylation of sphingolipid bases by Carter H. E. (1967)
Deuel H. J. Jr. 1951. The lipids vol. 1 p. 475-477. Interscience Publishers Inc. New York.
Gaver , R. C. , and C. C. Sweeley . 1965 . Methods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography . J. Amer. Oil Chem. Soc. 42 : 294 - 298 . (10.1007/BF02540132) / J. Amer. Oil Chem. Soc. / Methods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography by Gaver R. C. (1965)
Hancock , I. C. , G. 0. Humphreys , and P. M. Meadow . 1970 . Characterization of the hydroxy acids of Pseudomonas aeruginosa 8602 . Biochim. Biophys. Acta 202 : 389 - 391 . (10.1016/0005-2760(70)90204-3) / Biochim. Biophys. Acta / Characterization of the hydroxy acids of Pseudomonas aeruginosa 8602 by Hancock I. C. (1970)
Kunsman , J. E. 1973 . Characterization of the lipids of six strains of Bacteroides ruminicola . J. Bacteriol. 113 : 1121 - 1126 . (10.1128/jb.113.3.1121-1126.1973) / J. Bacteriol. / Characterization of the lipids of six strains of Bacteroides ruminicola by Kunsman J. E. (1973)
LaBach , J. P. , and D. C. White . 1969 . Identification of ceramide phosphorylethanolamine and ceramide phosphorylglycerol in the lipids of an anaerobic bacterium . J. Lipid Res. 10 : 528 - 534 . (10.1016/S0022-2275(20)43045-7) / J. Lipid Res. / Identification of ceramide phosphorylethanolamine and ceramide phosphorylglycerol in the lipids of an anaerobic bacterium by LaBach J. P. (1969)
Lennarz W. J. 1966. Lipid metabolism in the bacteria p. 175-225. In R. Paoletti and D. Kritchevsky (ed.) Advances in lipid research vol 4. Academic Press Inc. New York. (10.1016/B978-1-4831-9940-5.50012-0)
Majerus P. W. and P. R. Vagelos. 1967. Fatty acid biosynthesis and the role of acyl carrier protein p. 2-33. In R. Paoletti and D. Kritchevsky (ed.) Advances in lipid research vol. 5. Academic Press Inc. New York.
Plackett , P. , P. F. Smith , and W. R. Mayberry . 1970 . Lipids of a sterol non-requiring Mycoplasma . J. Bacteriol. 104 : 798 - 807 . (10.1128/jb.104.2.798-807.1970) / J. Bacteriol. / Lipids of a sterol non-requiring Mycoplasma by Plackett P. (1970)
Rizza , V. , A. N. Tucker , and D. C. White . 1970 . Lipids of Bacteroides melaninogenicus . J. Bacteriol. 101 : 84 - 91 . (10.1128/jb.101.1.84-91.1970) / J. Bacteriol. / Lipids of Bacteroides melaninogenicus by Rizza V. (1970)
Rottem , S. , 0. Muhsam-Peled , and S. Razin . 1973 . Acyl carrier protein in Mycoplasmas . J. Bacteriol. 113 : 586 - 591 . (10.1128/jb.113.2.586-591.1973) / J. Bacteriol. / Acyl carrier protein in Mycoplasmas by Rottem S. (1973)
Schriner R. L. 1942. Organic Reactions vol. 1 p. 16. John Wiley and Sons Inc. New York.
Tourtellotte , M. E. , R. G. Jensen , G. W. Gander , and H. J. Morowitz . 1963 . Lipid composition and synthesis in the pleuropneumonia-like organism Mycoplasma gallisepticum . J. Bacteriol. 86 : 370 - 379 . (10.1128/jb.86.3.370-379.1963) / J. Bacteriol. / Lipid composition and synthesis in the pleuropneumonia-like organism Mycoplasma gallisepticum by Tourtellotte M. E. (1963)
Tully , J. G. , and S. Razin . 1969 . Characteristics of a new sterol nonrequiring Mycoplasma . J. Bacteriol. 98 : 970 - 978 . (10.1128/jb.98.3.970-978.1969) / J. Bacteriol. / Characteristics of a new sterol nonrequiring Mycoplasma by Tully J. G. (1969)
Tully , J. G. , and S. Razin . 1970 . Acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the Mycoplasmatales . J. Bacteriol. 103 : 751 - 754 . (10.1128/jb.103.3.751-754.1970) / J. Bacteriol. / Acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the Mycoplasmatales by Tully J. G. (1970)
Wells , M. A. , and J. C. Dittmer . 1963 . The use of Sephadex for the removal of non-lipid contaminants from lipid extracts . Biochemistry 2 : 1259 - 1263 . (10.1021/bi00906a015) / Biochemistry / The use of Sephadex for the removal of non-lipid contaminants from lipid extracts by Wells M. A. (1963)
Wynne R. B. J. A. Schmidt and G. R. Umbreit. 1965. Perchloric acid catalyzed reactiona. I. Methylation of fatty acids. F and M Biomedical GC Notes F and M Scientific Corporation Avondale Pa.

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:53 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:10 p.m.)
Indexed	1 year, 10 months ago (Oct. 15, 2023, 10:51 a.m.)
Issued	51 years, 9 months ago (Dec. 1, 1973)
Published	51 years, 9 months ago (Dec. 1, 1973)
Published Print	51 years, 9 months ago (Dec. 1, 1973)

Funders 0

None

BibTeX

@article{Mayberry_1973, title={Identification of the Amide-Linked Fatty Acids of Acholeplasma axanthum S743 as <scp>d</scp> (–)3-Hydroxyhexadecanoate and Its Homologues}, volume={116}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.116.3.1091-1095.1973}, DOI={10.1128/jb.116.3.1091-1095.1973}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Mayberry, William R. and Smith, P. F. and Langworthy, Thomas A. and Plackett, Philip}, year={1973}, month=dec, pages={1091–1095} }

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