DOI: 10.1128/jb.115.1.426-435.1973. Peptidoglycan of Free-Living Anaerobic Spirochetes

Peptidoglycan of Free-Living Anaerobic Spirochetes

10.1128/jb.115.1.426-435.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Electron microscope examination of negatively stained or thin-sectioned cells of Spirochaeta stenostrepta treated with penicillin or lysozyme showed that the peptidoglycan was present as a thin, electron-dense layer adjacent and external to the cytoplasmic membrane. The peptidoglycan was isolated from cells of S. stenostrepta and Spirochaeta litoralis by a procedure including treatments with sodium lauryl sulfate and Pronase. Hydrolysates of the isolated S. stenostrepta and S. litoralis peptidoglycans contained glucosamine, muramic acid, glutamic acid, l -ornithine, and alanine in molar ratios of 0.90:0.85:1.00:1.00:1.40 and of 0.63:0.63:0.99:1.00:1.41, respectively. Determination of N-terminal residues suggested that nearly 50% of the ornithine in S. stenostrepta and S. litoralis peptidoglycans was involved in peptide cross-linkage. The peptidoglycan layer of S. stenostrepta was sensitive to lysozyme and myxobacter AL-1 protease.

Bibliography

Joseph, R., Holt, S. C., & Canale-Parola, E. (1973). Peptidoglycan of Free-Living Anaerobic Spirochetes. Journal of Bacteriology, 115(1), 426â435.

Authors 3

R. Joseph (first)
S. C. Holt (additional)
E. Canale-Parola (additional)

References 25 Referenced 40

Ames , B. N. , and D. T. Dubin . 1960 . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid . J. Biol. Chem. 235 : 769 - 775 . (10.1016/S0021-9258(19)67936-6) / J. Biol. Chem. / The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid by Ames B. N. (1960)
Chen , P. S. , T. Y. Toribara , and H. Warner . 1956 . Microdetermination of phosphorus . Anal. Chem. 28 : 1756 - 1758 . (10.1021/ac60119a033) / Anal. Chem. / Microdetermination of phosphorus by Chen P. S. (1956)
Chinard , F. P. 1952 . Photometric estimation of proline and omithine . J. Biol. Chem. 199 : 91 - 95 . (10.1016/S0021-9258(18)44814-4) / J. Biol. Chem. / Photometric estimation of proline and omithine by Chinard F. P. (1952)
Cummins , C. S. , and H. Harris . 1956 . The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character . J. Gen. Microbiol. 14 : 583 - 600 . (10.1099/00221287-14-3-583) / J. Gen. Microbiol. / The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character by Cummins C. S. (1956)
de Petris , S. 1965 . Ultrastructure of the cell wall of Escherichia coli . J. Ultrastruc. Res. 12 : 247 - 262 . (10.1016/S0022-5320(65)80098-3) / J. Ultrastruc. Res. / Ultrastructure of the cell wall of Escherichia coli by de Petris S. (1965)
Dubois , M. , K. A. Gilles , J. K. Hamilton , P. A. Rebers , and F. Smith . 1956 . Colorimetric method for determination of sugars and related substances . Anal. Chem. 28 : 350 - 356 . (10.1021/ac60111a017) / Anal. Chem. / Colorimetric method for determination of sugars and related substances by Dubois M. (1956)
Friedberg , I. , and G. Avigad . 1966 . High Iysozyme concentration and Iysis of Micrococcus Iysodeikticus. Biochim. Biophys. Acta 127 : 532 - 535 . (10.1016/0304-4165(66)90409-0) / Iysodeikticus. Biochim. Biophys. Acta / High Iysozyme concentration and Iysis of Micrococcus by Friedberg I. (1966)
Ghuysen J. M. D. J. Tipper and J. L. Strominger. 1966. Enzymes that degrade bacterial cell walls p. 685-699. In E. F. Neufeld and V. Ginsburg (ed.) Methods in enzymology vol. 8. Academic Press Inc. New York. (10.1016/0076-6879(66)08124-2)
Ginger , C. D. 1963 . Isolation and characterization of muramic acid from two spirochaetes: Borrelia duttoni and Leptospira biflexa . Nature (London) 199 : 159 . (10.1038/199159a0) / Nature (London) / Isolation and characterization of muramic acid from two spirochaetes: Borrelia duttoni and Leptospira biflexa by Ginger C. D. (1963)
Holt , S. C. , and E. Canale-Parola . 1968 . Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete . J. Bacteriol. 96 : 822 - 835 . (10.1128/jb.96.3.822-835.1968) / J. Bacteriol. / Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete by Holt S. C. (1968)
Hunninghake , D. , and S. Grisolia . 1966 . A sensitive and convenient micro-method for estimation of urea, citrulline, and carbamyl derivatives . Anal. Biochem. 16 : 200 - 205 . (10.1016/0003-2697(66)90147-3) / Anal. Biochem. / A sensitive and convenient micro-method for estimation of urea, citrulline, and carbamyl derivatives by Hunninghake D. (1966)
Jones M. E. 1962. Carbamyl phosphate synthesis and utilization p. 903-925. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 5. Academic Press Inc. New York. (10.1016/S0076-6879(62)05336-7)
Joseph , R. , and E. Canale-Parola . 1972 . Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics . Arch. Mikrobiol. 81 : 146 - 168 . (10.1007/BF00412325) / Arch. Mikrobiol. / Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics by Joseph R. (1972)
Kolenbrander , P. E. , and J. C. Ensign . 1968 . Isolation and chemical structure of the peptidoglycan of Spirillum serpens cell walls . J. Bacteriol. 95 : 201 - 210 . (10.1128/jb.95.1.201-210.1968) / J. Bacteriol. / Isolation and chemical structure of the peptidoglycan of Spirillum serpens cell walls by Kolenbrander P. E. (1968)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Mandelstam , J. 1962 . Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria . Biochem. J. 84 : 294 - 299 . (10.1042/bj0840294) / Biochem. J. / Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria by Mandelstam J. (1962)
Murray , R. G. E. , P. Steed , and H. E. Elson . 1965 . The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria . Can. J. Microbiol. 11 : 547 - 560 . (10.1139/m65-072) / Can. J. Microbiol. / The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria by Murray R. G. E. (1965)
Perkins , H. R. , and H. J. Rogers . 1959 . The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus Iysodeikticus . Biochem. J. 72 : 647 - 654 . (10.1042/bj0720647) / Biochem. J. / The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus Iysodeikticus by Perkins H. R. (1959)
Pillot , J. , and A. Ryter . 1965 . Structure des spirochbtes: I. Etude des genres Treponema, Borrelia, et Leptospira au microscope electronique . Ann. Inst. Pasteur 108 : 791 - 804 . / Ann. Inst. Pasteur / Structure des spirochbtes: I. Etude des genres Treponema, Borrelia, et Leptospira au microscope electronique by Pillot J. (1965)
Schocher , A. J. , S. T. Bayley , and R. W. Watson . 1962 . Composition of purified mucopeptide from the wall of Aerobacter cloacae . Can. J. Microbiol. 8 : 89 - 98 . (10.1139/m62-012) / Can. J. Microbiol. / Composition of purified mucopeptide from the wall of Aerobacter cloacae by Schocher A. J. (1962)
Spies J. R. 1957. Colorimetric procedures for amino acids. p. 467-477. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 3. Academic Press Inc. New York. (10.1016/S0076-6879(57)03417-5)
Tinelli , R. , and J. Pillot . 1966 . Ktude de la composition du glycopeptide de Treponema reiteri . C. R. Acad. Sci. Paris 263 : 739 - 741 . / C. R. Acad. Sci. Paris / Ktude de la composition du glycopeptide de Treponema reiteri by Tinelli R. (1966)
Weidel , W. , H. Frank , and H. H. Martin . 1960 . The rigid layer of the cell wall of Escherichia coli strain -B . J. Gen. Microbiol. 22 : 158 - 166 . (10.1099/00221287-22-1-158) / J. Gen. Microbiol. / The rigid layer of the cell wall of Escherichia coli strain -B by Weidel W. (1960)
Wheat R. W. 1966. Analysis of hexosamines in bacterial polysaccharides by chromatographic procedures p. 60-78. In E. F. Neufeld and V. Ginsburg (ed.) Methods in enzymology vol. 8. Academic Press Inc. New York. (10.1016/0076-6879(66)08008-X)
Yanagawa , R. , and S. Faine . 1966 . Morphological and serological analysis of leptospiral structure . Nature (London) 211 : 823 - 826 . (10.1038/211823a0) / Nature (London) / Morphological and serological analysis of leptospiral structure by Yanagawa R. (1966)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:53 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:08 p.m.)
Indexed	1 year, 5 months ago (March 12, 2024, 8:25 a.m.)
Issued	52 years, 1 month ago (July 1, 1973)
Published	52 years, 1 month ago (July 1, 1973)
Published Print	52 years, 1 month ago (July 1, 1973)

Funders 0

None

BibTeX

@article{Joseph_1973, title={Peptidoglycan of Free-Living Anaerobic Spirochetes}, volume={115}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.115.1.426-435.1973}, DOI={10.1128/jb.115.1.426-435.1973}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Joseph, R. and Holt, S. C. and Canale-Parola, E.}, year={1973}, month=jul, pages={426–435} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        3,
        12
      ]
    ],
    "date-time": "2024-03-12T12:25:59Z",
    "timestamp": 1710246359914
  },
  "reference-count": 25,
  "publisher": "American Society for Microbiology",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1973,
            7,
            1
          ]
        ],
        "date-time": "1973-07-01T00:00:00Z",
        "timestamp": 110332800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  },
  "abstract": "<jats:p>\n            Electron microscope examination of negatively stained or thin-sectioned cells of\n            <jats:italic>Spirochaeta stenostrepta</jats:italic>\n            treated with penicillin or lysozyme showed that the peptidoglycan was present as a thin, electron-dense layer adjacent and external to the cytoplasmic membrane. The peptidoglycan was isolated from cells of\n            <jats:italic>S. stenostrepta</jats:italic>\n            and\n            <jats:italic>Spirochaeta litoralis</jats:italic>\n            by a procedure including treatments with sodium lauryl sulfate and Pronase. Hydrolysates of the isolated\n            <jats:italic>S. stenostrepta</jats:italic>\n            and\n            <jats:italic>S. litoralis</jats:italic>\n            peptidoglycans contained glucosamine, muramic acid, glutamic acid,\n            <jats:sc>l</jats:sc>\n            -ornithine, and alanine in molar ratios of 0.90:0.85:1.00:1.00:1.40 and of 0.63:0.63:0.99:1.00:1.41, respectively. Determination of N-terminal residues suggested that nearly 50% of the ornithine in\n            <jats:italic>S. stenostrepta</jats:italic>\n            and\n            <jats:italic>S. litoralis</jats:italic>\n            peptidoglycans was involved in peptide cross-linkage. The peptidoglycan layer of\n            <jats:italic>S. stenostrepta</jats:italic>\n            was sensitive to lysozyme and myxobacter AL-1 protease.\n          </jats:p>",
  "DOI": "10.1128/jb.115.1.426-435.1973",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T14:53:09Z",
    "timestamp": 1578063189000
  },
  "page": "426-435",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 40,
  "title": "Peptidoglycan of Free-Living Anaerobic Spirochetes",
  "prefix": "10.1128",
  "volume": "115",
  "author": [
    {
      "given": "R.",
      "family": "Joseph",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002"
        }
      ]
    },
    {
      "given": "S. C.",
      "family": "Holt",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002"
        }
      ]
    },
    {
      "given": "E.",
      "family": "Canale-Parola",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "769",
      "DOI": "10.1016/S0021-9258(19)67936-6",
      "article-title": "The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid",
      "volume": "235",
      "author": "Ames B. N.",
      "year": "1960",
      "unstructured": "Ames , B. N. , and D. T. Dubin . 1960 . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid . J. Biol. Chem. 235 : 769 - 775 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_20",
      "doi-asserted-by": "crossref",
      "first-page": "1756",
      "DOI": "10.1021/ac60119a033",
      "article-title": "Microdetermination of phosphorus",
      "volume": "28",
      "author": "Chen P. S.",
      "year": "1956",
      "unstructured": "Chen , P. S. , T. Y. Toribara , and H. Warner . 1956 . Microdetermination of phosphorus . Anal. Chem. 28 : 1756 - 1758 .",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "91",
      "DOI": "10.1016/S0021-9258(18)44814-4",
      "article-title": "Photometric estimation of proline and omithine",
      "volume": "199",
      "author": "Chinard F. P.",
      "year": "1952",
      "unstructured": "Chinard , F. P. 1952 . Photometric estimation of proline and omithine . J. Biol. Chem. 199 : 91 - 95 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "583",
      "DOI": "10.1099/00221287-14-3-583",
      "article-title": "The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character",
      "volume": "14",
      "author": "Cummins C. S.",
      "year": "1956",
      "unstructured": "Cummins , C. S. , and H. Harris . 1956 . The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character . J. Gen. Microbiol. 14 : 583 - 600 .",
      "journal-title": "J. Gen. Microbiol."
    },
    {
      "key": "p_23",
      "doi-asserted-by": "crossref",
      "first-page": "247",
      "DOI": "10.1016/S0022-5320(65)80098-3",
      "article-title": "Ultrastructure of the cell wall of Escherichia coli",
      "volume": "12",
      "author": "de Petris S.",
      "year": "1965",
      "unstructured": "de Petris , S. 1965 . Ultrastructure of the cell wall of Escherichia coli . J. Ultrastruc. Res. 12 : 247 - 262 .",
      "journal-title": "J. Ultrastruc. Res."
    },
    {
      "key": "p_24",
      "doi-asserted-by": "crossref",
      "first-page": "350",
      "DOI": "10.1021/ac60111a017",
      "article-title": "Colorimetric method for determination of sugars and related substances",
      "volume": "28",
      "author": "Dubois M.",
      "year": "1956",
      "unstructured": "Dubois , M. , K. A. Gilles , J. K. Hamilton , P. A. Rebers , and F. Smith . 1956 . Colorimetric method for determination of sugars and related substances . Anal. Chem. 28 : 350 - 356 .",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "p_25",
      "doi-asserted-by": "crossref",
      "first-page": "532",
      "DOI": "10.1016/0304-4165(66)90409-0",
      "article-title": "High Iysozyme concentration and Iysis of Micrococcus",
      "volume": "127",
      "author": "Friedberg I.",
      "year": "1966",
      "unstructured": "Friedberg , I. , and G. Avigad . 1966 . High Iysozyme concentration and Iysis of Micrococcus Iysodeikticus. Biochim. Biophys. Acta 127 : 532 - 535 .",
      "journal-title": "Iysodeikticus. Biochim. Biophys. Acta"
    },
    {
      "key": "p_26",
      "doi-asserted-by": "crossref",
      "unstructured": "Ghuysen J. M. D. J. Tipper and J. L. Strominger. 1966. Enzymes that degrade bacterial cell walls p. 685-699. In E. F. Neufeld and V. Ginsburg (ed.) Methods in enzymology vol. 8. Academic Press Inc. New York.",
      "DOI": "10.1016/0076-6879(66)08124-2"
    },
    {
      "key": "p_27",
      "doi-asserted-by": "crossref",
      "first-page": "159",
      "DOI": "10.1038/199159a0",
      "article-title": "Isolation and characterization of muramic acid from two spirochaetes: Borrelia duttoni and Leptospira biflexa",
      "volume": "199",
      "author": "Ginger C. D.",
      "year": "1963",
      "unstructured": "Ginger , C. D. 1963 . Isolation and characterization of muramic acid from two spirochaetes: Borrelia duttoni and Leptospira biflexa . Nature (London) 199 : 159 .",
      "journal-title": "Nature (London)"
    },
    {
      "key": "p_28",
      "doi-asserted-by": "crossref",
      "first-page": "822",
      "DOI": "10.1128/jb.96.3.822-835.1968",
      "article-title": "Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete",
      "volume": "96",
      "author": "Holt S. C.",
      "year": "1968",
      "unstructured": "Holt , S. C. , and E. Canale-Parola . 1968 . Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete . J. Bacteriol. 96 : 822 - 835 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_29",
      "doi-asserted-by": "crossref",
      "first-page": "200",
      "DOI": "10.1016/0003-2697(66)90147-3",
      "article-title": "A sensitive and convenient micro-method for estimation of urea, citrulline, and carbamyl derivatives",
      "volume": "16",
      "author": "Hunninghake D.",
      "year": "1966",
      "unstructured": "Hunninghake , D. , and S. Grisolia . 1966 . A sensitive and convenient micro-method for estimation of urea, citrulline, and carbamyl derivatives . Anal. Biochem. 16 : 200 - 205 .",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "p_30",
      "doi-asserted-by": "crossref",
      "unstructured": "Jones M. E. 1962. Carbamyl phosphate synthesis and utilization p. 903-925. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 5. Academic Press Inc. New York.",
      "DOI": "10.1016/S0076-6879(62)05336-7"
    },
    {
      "key": "p_31",
      "doi-asserted-by": "crossref",
      "first-page": "146",
      "DOI": "10.1007/BF00412325",
      "article-title": "Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics",
      "volume": "81",
      "author": "Joseph R.",
      "year": "1972",
      "unstructured": "Joseph , R. , and E. Canale-Parola . 1972 . Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics . Arch. Mikrobiol. 81 : 146 - 168 .",
      "journal-title": "Arch. Mikrobiol."
    },
    {
      "key": "p_32",
      "doi-asserted-by": "crossref",
      "first-page": "201",
      "DOI": "10.1128/jb.95.1.201-210.1968",
      "article-title": "Isolation and chemical structure of the peptidoglycan of Spirillum serpens cell walls",
      "volume": "95",
      "author": "Kolenbrander P. E.",
      "year": "1968",
      "unstructured": "Kolenbrander , P. E. , and J. C. Ensign . 1968 . Isolation and chemical structure of the peptidoglycan of Spirillum serpens cell walls . J. Bacteriol. 95 : 201 - 210 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_33",
      "doi-asserted-by": "crossref",
      "first-page": "265",
      "DOI": "10.1016/S0021-9258(19)52451-6",
      "article-title": "Protein measurement with the Folin phenol reagent",
      "volume": "193",
      "author": "Lowry 0.",
      "year": "1951",
      "unstructured": "Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_34",
      "doi-asserted-by": "crossref",
      "first-page": "294",
      "DOI": "10.1042/bj0840294",
      "article-title": "Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria",
      "volume": "84",
      "author": "Mandelstam J.",
      "year": "1962",
      "unstructured": "Mandelstam , J. 1962 . Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria . Biochem. J. 84 : 294 - 299 .",
      "journal-title": "Biochem. J."
    },
    {
      "key": "p_35",
      "doi-asserted-by": "crossref",
      "first-page": "547",
      "DOI": "10.1139/m65-072",
      "article-title": "The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria",
      "volume": "11",
      "author": "Murray R. G. E.",
      "year": "1965",
      "unstructured": "Murray , R. G. E. , P. Steed , and H. E. Elson . 1965 . The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria . Can. J. Microbiol. 11 : 547 - 560 .",
      "journal-title": "Can. J. Microbiol."
    },
    {
      "key": "p_36",
      "doi-asserted-by": "crossref",
      "first-page": "647",
      "DOI": "10.1042/bj0720647",
      "article-title": "The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus Iysodeikticus",
      "volume": "72",
      "author": "Perkins H. R.",
      "year": "1959",
      "unstructured": "Perkins , H. R. , and H. J. Rogers . 1959 . The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus Iysodeikticus . Biochem. J. 72 : 647 - 654 .",
      "journal-title": "Biochem. J."
    },
    {
      "key": "p_37",
      "first-page": "791",
      "article-title": "Structure des spirochbtes: I. Etude des genres Treponema, Borrelia, et Leptospira au microscope electronique",
      "volume": "108",
      "author": "Pillot J.",
      "year": "1965",
      "unstructured": "Pillot , J. , and A. Ryter . 1965 . Structure des spirochbtes: I. Etude des genres Treponema, Borrelia, et Leptospira au microscope electronique . Ann. Inst. Pasteur 108 : 791 - 804 .",
      "journal-title": "Ann. Inst. Pasteur"
    },
    {
      "key": "p_38",
      "doi-asserted-by": "crossref",
      "first-page": "89",
      "DOI": "10.1139/m62-012",
      "article-title": "Composition of purified mucopeptide from the wall of Aerobacter cloacae",
      "volume": "8",
      "author": "Schocher A. J.",
      "year": "1962",
      "unstructured": "Schocher , A. J. , S. T. Bayley , and R. W. Watson . 1962 . Composition of purified mucopeptide from the wall of Aerobacter cloacae . Can. J. Microbiol. 8 : 89 - 98 .",
      "journal-title": "Can. J. Microbiol."
    },
    {
      "key": "p_39",
      "doi-asserted-by": "crossref",
      "unstructured": "Spies J. R. 1957. Colorimetric procedures for amino acids. p. 467-477. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 3. Academic Press Inc. New York.",
      "DOI": "10.1016/S0076-6879(57)03417-5"
    },
    {
      "key": "p_40",
      "first-page": "739",
      "article-title": "Ktude de la composition du glycopeptide de Treponema reiteri",
      "volume": "263",
      "author": "Tinelli R.",
      "year": "1966",
      "unstructured": "Tinelli , R. , and J. Pillot . 1966 . Ktude de la composition du glycopeptide de Treponema reiteri . C. R. Acad. Sci. Paris 263 : 739 - 741 .",
      "journal-title": "C. R. Acad. Sci. Paris"
    },
    {
      "key": "p_41",
      "doi-asserted-by": "crossref",
      "first-page": "158",
      "DOI": "10.1099/00221287-22-1-158",
      "article-title": "The rigid layer of the cell wall of Escherichia coli strain -B",
      "volume": "22",
      "author": "Weidel W.",
      "year": "1960",
      "unstructured": "Weidel , W. , H. Frank , and H. H. Martin . 1960 . The rigid layer of the cell wall of Escherichia coli strain -B . J. Gen. Microbiol. 22 : 158 - 166 .",
      "journal-title": "J. Gen. Microbiol."
    },
    {
      "key": "p_42",
      "doi-asserted-by": "crossref",
      "unstructured": "Wheat R. W. 1966. Analysis of hexosamines in bacterial polysaccharides by chromatographic procedures p. 60-78. In E. F. Neufeld and V. Ginsburg (ed.) Methods in enzymology vol. 8. Academic Press Inc. New York.",
      "DOI": "10.1016/0076-6879(66)08008-X"
    },
    {
      "key": "p_43",
      "doi-asserted-by": "crossref",
      "first-page": "823",
      "DOI": "10.1038/211823a0",
      "article-title": "Morphological and serological analysis of leptospiral structure",
      "volume": "211",
      "author": "Yanagawa R.",
      "year": "1966",
      "unstructured": "Yanagawa , R. , and S. Faine . 1966 . Morphological and serological analysis of leptospiral structure . Nature (London) 211 : 823 - 826 .",
      "journal-title": "Nature (London)"
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.115.1.426-435.1973",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.115.1.426-435.1973",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:08:00Z",
    "timestamp": 1627578480000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.115.1.426-435.1973"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  },
  "references-count": 25,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1973,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.115.1.426-435.1973"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.115.1.426-435.1973",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}