DOI: 10.1128/jb.115.1.198-204.1973. Common Enzymes of Branched-Chain Amino Acid Catabolism in <i>Pseudomonas putida</i>

Common Enzymes of Branched-Chain Amino Acid Catabolism in Pseudomonas putida

10.1128/jb.115.1.198-204.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Two types of Pseudomonas putida PpG2 mutants which were unable to degrade branched-chain amino acids were isolated after mutagenesis and selection for ability to grow on succinate, but not valine, as a sole source of carbon. These isolates were characterized by growth on the three branched-chain amino acids (valine, isoleucine, and leucine), on the corresponding branched-chain keto acids (2-ketoisovalerate, 2-keto-3-methylvalerate, and 2-ketoisocaproate), and on other selected intermediates as carbon sources, and by their enzymatic composition. One group of mutants lost 2-ketoisovalerate-inducible branched-chain keto acid dehydrogenase that was active on all three keto acids. There was also a concomitant loss of ability to grow on all three branched-chain amino acids as well as on all three corresponding keto acids, but there was retention of ability to use subsequent intermediates in the catabolism of branched-chain amino acids. Another type of mutant showed a marked reduction in branched-chain amino acid transaminase activity and grew poorly at the expense of all three amino acids, but it utilized subsequent intermediates as carbon sources. Both the transaminase and branched-chain keto acid dehydrogenase mutants retained the ability to degrade camphor. These findings are consistent with the view that branched-chain amino acid transaminase and branched-chain keto acid dehydrogenase are common enzymes in the catabolism of valine, isoleucine, and leucine.

Bibliography

Martin, R. R., Marshall, V. D., Sokatch, J. R., & Unger, L. (1973). Common Enzymes of Branched-Chain Amino Acid Catabolism in Pseudomonas putida. Journal of Bacteriology, 115(1), 198â204.

Authors 4

Ronald R. Martin (first)
Vincent D. Marshall (additional)
John R. Sokatch (additional)
Leon Unger (additional)

References 19 Referenced 41

Adelberg , E. A. , M. Mandel , and G. C. C. Chen . 1965 . Optimal conditions for mutagenesis by N-methyl-N'- nitro-N-nitrosoguanidine in Escherichia coli K12 . Biochem. Biophys. Res. Commun. 18 : 788 - 795 . (10.1016/0006-291X(65)90855-7) / Biochem. Biophys. Res. Commun. / Optimal conditions for mutagenesis by N-methyl-N'- nitro-N-nitrosoguanidine in Escherichia coli K12 by Adelberg E. A. (1965)
Demerec , M. , E. A. Adelberg , A. J. Clark , and P. E. Hartman . 1966 . A proposal for a uniform nomenclature in bacterial genetics . Genetics 54 : 61 - 76 . (10.1093/genetics/54.1.61) / Genetics / A proposal for a uniform nomenclature in bacterial genetics by Demerec M. (1966)
Jacobson , L. A. , R. C. Bartholomaus , and I. C. Gunsalus . 1966 . Repression of malic enzyme by acetate in Pseudomonas . Biochem. Biophys. Res. Commun. 24 : 955 - 960 . (10.1016/0006-291X(66)90343-3) / Biochem. Biophys. Res. Commun. / Repression of malic enzyme by acetate in Pseudomonas by Jacobson L. A. (1966)
Kay , W. W. , and A. F. Gronlund . 1969 . Amino acid transport in Pseudomonas aeruginosa . J. Bacteriol. 97 : 273 - 281 . (10.1128/JB.97.1.273-281.1969) / J. Bacteriol. / Amino acid transport in Pseudomonas aeruginosa by Kay W. W. (1969)
Kerwar , S. S. , V. H. Cheldelin , and L. W. Parks . 1964 . Valine-isoleucine metabolism in Acetobacter suboxydans and the inhibition of growth by valine . J. Bacteriol. 88 : 179 - 186 . (10.1128/JB.88.1.179-186.1964) / J. Bacteriol. / Valine-isoleucine metabolism in Acetobacter suboxydans and the inhibition of growth by valine by Kerwar S. S. (1964)
Loveless , A. , and S. Howarth . 1959 . Mutation of bacteria at high levels of survival by ethyl methane sulphonate . Nature (London) 184 : 1780 - 1782 . (10.1038/1841780a0) / Nature (London) / Mutation of bacteria at high levels of survival by ethyl methane sulphonate by Loveless A. (1959)
Marinus , M. G. , and J. S. Loutit . 1969 . Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterization and mapping of mutants . Genetics 63 : 547 - 556 . (10.1093/genetics/63.3.547) / Genetics / Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterization and mapping of mutants by Marinus M. G. (1969)
Marshall , V. P. , and J. R. Sokatch . 1968 . Oxidation of D-amino acids by a particulate enzyme from Pseudomonas aeruginosa . J. Bacteriol. 95 : 1419 - 1424 . (10.1128/jb.95.4.1419-1424.1968) / J. Bacteriol. / Oxidation of D-amino acids by a particulate enzyme from Pseudomonas aeruginosa by Marshall V. P. (1968)
Marshall , V. P. , and J. R. Sokatch . 1972 . Regulation of valine catabolism in Pseudomonas putida . J. Bacteriol. 110 : 1073 - 1081 . (10.1128/jb.110.3.1073-1081.1972) / J. Bacteriol. / Regulation of valine catabolism in Pseudomonas putida by Marshall V. P. (1972)
Norton , J: E. , G. S. Bulmer , and J. R. Sokatch . 1963 . The oxidation of D-alanine by cell membranes of Pseudomonas aeruginosa . Biochim. Biophys. Acta 78 : 136 - 147 . (10.1016/0006-3002(63)91619-6) / Biochim. Biophys. Acta / The oxidation of D-alanine by cell membranes of Pseudomonas aeruginosa by Norton E. (1963)
Norton , J. E. , and J. R. Sokatch . 1970 . Purification and partial characterization of the branched-chain amino acid transaminase of Pseudomonas aeruginosa . Biochim. Biophys. Acta 206 : 261 - 269 . (10.1016/0005-2744(70)90109-9) / Biochim. Biophys. Acta / Purification and partial characterization of the branched-chain amino acid transaminase of Pseudomonas aeruginosa by Norton J. E. (1970)
Ramakrishnan , T. , and E. A. Adelberg . 1964 . Regulatory mechanisms in the biosynthesis of isoleucine and valine. I. Genetic derepression of enzyme formation . J. Bacteriol. 87 : 566 - 573 . (10.1128/JB.87.3.566-573.1964) / J. Bacteriol. / Regulatory mechanisms in the biosynthesis of isoleucine and valine. I. Genetic derepression of enzyme formation by Ramakrishnan T. (1964)
Robinson , W. G. , and M. J. Coon . 1957 . The purification and properties of ,-hydroxyisobutyric dehydrogenase . J. Biol. Chem. 225 : 511 - 521 . (10.1016/S0021-9258(18)64948-8) / J. Biol. Chem. / The purification and properties of ,-hydroxyisobutyric dehydrogenase by Robinson W. G. (1957)
Rogerson , A. C. , and M. Freundlich . 1970 . Control of isoleucine, valine and leucine biosynthesis. VIm. Mechanism of growth inhibition by leucine in relaxed and stringent strains of E. coli K12 . Biochim. Biophys. Acta 208 : 87 - 98 . (10.1016/0304-4165(70)90051-6) / Biochim. Biophys. Acta / Control of isoleucine, valine and leucine biosynthesis. VIm. Mechanism of growth inhibition by leucine in relaxed and stringent strains of E. coli K12 by Rogerson A. C. (1970)
Sokatch , J. R. , L. E. Sanders , and V. P. Marshall . 1968 . Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine . J. Biol. Chem. 243 : 2500 - 2506 . (10.1016/S0021-9258(18)93403-4) / J. Biol. Chem. / Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine by Sokatch J. R. (1968)
Taylor , R. T. , and W. T. Jenkins . 1966 . Leucine amino transferase. I. Colorimetric assays . J. Biol. Chem. 241 : 4391 - 4395 . / J. Biol. Chem. / Leucine amino transferase. I. Colorimetric assays by Taylor R. T. (1966)
Umbarger , H. E. , and B. Brown . 1955 . Isoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine . J. Bacteriol. 70 : 241 - 248 . (10.1128/JB.70.2.241-248.1955) / J. Bacteriol. / Isoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine by Umbarger H. E. (1955)
Vogel , H. J. , and D. M. Bonner . 1956 . Acetylornithinase of E. coli: partial purification and some properties . J. Biol. Chem. 218 : 97 - 106 . (10.1016/S0021-9258(18)65874-0) / J. Biol. Chem. / Acetylornithinase of E. coli: partial purification and some properties by Vogel H. J. (1956)
Warburg , O. , and W. Christian . 1941 . Isolierung und Kristallisation des Garungsferments Enolase . Biochem. Z. 310 : 384 - 421 . / Biochem. Z. / Isolierung und Kristallisation des Garungsferments Enolase by Warburg O. (1941)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:52 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:07 p.m.)
Indexed	4 months, 2 weeks ago (April 11, 2025, 2:10 a.m.)
Issued	52 years, 1 month ago (July 1, 1973)
Published	52 years, 1 month ago (July 1, 1973)
Published Print	52 years, 1 month ago (July 1, 1973)

Funders 0

None

BibTeX

@article{Martin_1973, title={Common Enzymes of Branched-Chain Amino Acid Catabolism in Pseudomonas putida}, volume={115}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.115.1.198-204.1973}, DOI={10.1128/jb.115.1.198-204.1973}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Martin, Ronald R. and Marshall, Vincent D. and Sokatch, John R. and Unger, Leon}, year={1973}, month=jul, pages={198–204} }

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