DOI: 10.1128/jb.114.3.1014-1017.1973. Deoxyribonucleic Acid Synthesis in recA and recB Derivatives of an Escherichia coli K-12 Strain with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I

Deoxyribonucleic Acid Synthesis in recA and recB Derivatives of an Escherichia coli K-12 Strain with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I

10.1128/jb.114.3.1014-1017.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

recA and recB derivatives of a strain of Escherichia coli with a temperature-sensitive deoxyribonucleic acid (DNA) polymerase I ( polA12 ) are inviable at high temperature, but continue to incorporate 3 H-thymine into DNA for extended periods. The DNA made in pulse-chase experiments at high temperature in the polA12 parent and its double-mutant derivatives has been examined by alkaline sucrose gradient sedimentation analysis. The low-molecular-weight DNA fragments made during short pulses were joined at the same rate in each strain. Furthermore, the resulting high-molecular-weight DNA was of the same size in each case and was stable for at least 50 min. It is concluded that the inviability of the double mutants is due neither to a defect in converting low-molecular-weight DNA intermediates to high molecular weight nor to the presence of unrepaired random breaks in their DNA.

Bibliography

Monk, M., Kinross, J., & Town, C. (1973). Deoxyribonucleic Acid Synthesis in recA and recB Derivatives of an Escherichia coli K-12 Strain with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I. Journal of Bacteriology, 114(3), 1014â1017.

Authors 3

M. Monk (first)
J. Kinross (additional)
C. Town (additional)

References 21 Referenced 13

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Gross , J. D. , J. Grunstein , and E. M. Witkin . 1971 . Inviability of recA- derivatives of the DNA polymerase mutant of de Lucia and Cairns . J. Mol. Biol. 58 : 631 - 634 . (10.1016/0022-2836(71)90377-9) / J. Mol. Biol. / Inviability of recA- derivatives of the DNA polymerase mutant of de Lucia and Cairns by Gross J. D. (1971)
Inouye , M. 1971 . Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication . J. Bacteriol. 106 : 539 - 542 . (10.1128/jb.106.2.539-542.1971) / J. Bacteriol. / Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication by Inouye M. (1971)
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Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:43 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:08 p.m.)
Indexed	3 years, 5 months ago (March 29, 2022, 8:51 a.m.)
Issued	52 years, 3 months ago (June 1, 1973)
Published	52 years, 3 months ago (June 1, 1973)
Published Print	52 years, 3 months ago (June 1, 1973)

Funders 0

None

BibTeX

@article{Monk_1973, title={Deoxyribonucleic Acid Synthesis in recA and recB Derivatives of an Escherichia coli K-12 Strain with a Temperature-Sensitive Deoxyribonucleic Acid Polymerase I}, volume={114}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.114.3.1014-1017.1973}, DOI={10.1128/jb.114.3.1014-1017.1973}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Monk, M. and Kinross, J. and Town, C.}, year={1973}, month=jun, pages={1014–1017} }

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