DOI: 10.1128/jb.113.1.105-113.1973. Genetic Analysis of Flagellar Mutants in<i>Escherichia coli</i>

Genetic Analysis of Flagellar Mutants inEscherichia coli

10.1128/jb.113.1.105-113.1973

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Flagellar mutants inEscherichia coliwere obtained by selection for resistance to the flagellotropic phage χ. F elements covering various regions of theE. coligenome were then constructed, and, on the basis of the ability of these elements to restore flagellar function, the mutations were assigned to three regions of theE. colichromosome. Region I is betweentrpandgal;region II is betweenuvrCandaroD;and region III is betweenhisanduvrC. F elements carrying flagellar mutations were constructed. Stable merodiploid strains with a flagellar defect on the exogenote and another on the endogenote were then prepared. These merodiploids yielded information on the complementation behavior of mutations in a given region. Region III was shown to include at least six cistrons,A, B, C, D, E, andF. Region II was shown to include at least four cistrons,G, H, I, andJ. Examination of the phenotypes of the mutants revealed that those with lesions in cistronEof region III produce “polyhooks” and lesions in cistronFof region III result in loss of ability to produce flagellin. Mutants with lesions in cistronJof region II were entirely paralyzed (mot) mutants. Genetic analysis of flagellar mutations in region III suggested that the mutations located in cistronsA, B, C, andEare closely linked and mutations in cistronsDandFare closely linked.

Bibliography

Silverman, M., & Simon, M. (1973). Genetic Analysis of Flagellar Mutants inEscherichia coli. Journal of Bacteriology, 113(1), 105â113.

Authors 2

M. Silverman (first)
M. Simon (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:40 a.m.)
Deposited	1 year, 11 months ago (Sept. 24, 2023, 1:30 p.m.)
Indexed	1 year, 11 months ago (Sept. 25, 2023, 5:52 a.m.)
Issued	52 years, 8 months ago (Jan. 1, 1973)
Published	52 years, 8 months ago (Jan. 1, 1973)
Published Print	52 years, 8 months ago (Jan. 1, 1973)

Funders 0

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BibTeX

@article{Silverman_1973, title={Genetic Analysis of Flagellar Mutants inEscherichia coli}, volume={113}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.113.1.105-113.1973}, DOI={10.1128/jb.113.1.105-113.1973}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Silverman, M. and Simon, M.}, year={1973}, month=jan, pages={105–113} }

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