DOI: 10.1128/jb.112.1.585-592.1972. Specific Removal of Proteins from the Envelope of <i>Escherichia coli</i> by Protease Treatments

Specific Removal of Proteins from the Envelope of Escherichia coli by Protease Treatments

10.1128/jb.112.1.585-592.1972

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

When the envelope fraction of Escherichia coli was treated by trypsin, about 40% of total envelope proteins were removed from the fraction without changing its phospholipid content. Analysis of envelope proteins by acrylamide gel electrophoresis in 0.5% sodium dodecyl sulfate revealed that trypsin treatment was very specific; one of the major proteins (molecular weight, 38,000) and all proteins of molecular weight greater than 70,000 were completely removed by the treatment. On the other hand, three other major proteins were found to be resistant to the treatment, including protein Y, which was previously shown to be related to deoxyribonucleic acid replication. The trypsin treatment of the envelope fractions composed of a five electron-dense layered structure formed vesicles with a triple-layered membrane (two electron-dense layers). Pronase treatment of the envelope fraction removed about 60% of the envelope proteins without changing its phospholipid content. A major protein of molecular weight of 58,000 was found to be the only protein resistant to the Pronase treatment. Application of these treatments is useful for purification and structural studies of envelope proteins.

Bibliography

Inouye, M., & Yee, M.-L. (1972). Specific Removal of Proteins from the Envelope of Escherichia coli by Protease Treatments. Journal of Bacteriology, 112(1), 585â592.

Authors 2

Masayori Inouye (first)
Mei-Lan Yee (additional)

References 19 Referenced 32

Bayer , M. E. , and C. C. Remsen . 1970 . Structure of Escherichia coli after freeze-etching . J. Bacteriol. 101 : 304 - 313 . (10.1128/jb.101.1.304-313.1970) / J. Bacteriol. / Structure of Escherichia coli after freeze-etching by Bayer M. E. (1970)
Braun , V. , and K. Rehn . 1969 . Chemical characterization, special distribution and function of a lipoprotein of the E. coli cell wall . Eur. J. Biochem. 10 : 426 - 438 . (10.1111/j.1432-1033.1969.tb00707.x) / Eur. J. Biochem. / Chemical characterization, special distribution and function of a lipoprotein of the E. coli cell wall by Braun V. (1969)
Braun , V. , and U. Sieglin . 1970 . The covalent mureinlipoprotein structure of the Escherichia coli cell wall . Eur. J. Biochem. 13 : 336 - 346 . (10.1111/j.1432-1033.1970.tb00936.x) / Eur. J. Biochem. / The covalent mureinlipoprotein structure of the Escherichia coli cell wall by Braun V. (1970)
Braun , V. , and H. Wolff . 1970 . The murein-lipoprotein linkage in the cell wall of Escherichia coli . Eur. J. Biochem. 14 : 387 - 391 . (10.1111/j.1432-1033.1970.tb00301.x) / Eur. J. Biochem. / The murein-lipoprotein linkage in the cell wall of Escherichia coli by Braun V. (1970)
De Pamphilis , M. L. , and J. Adler . 1971 . Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer lipopolysaccharide membrane and cytoplasmic membrane . J. Bacteriol. 105 : 396 - 407 . (10.1128/jb.105.1.396-407.1971) / J. Bacteriol. / Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer lipopolysaccharide membrane and cytoplasmic membrane by De Pamphilis M. L. (1971)
De Petris , S. 1967 . Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers . J. Ultrastruct. Res. 19 : 45 - 83 . (10.1016/S0022-5320(67)80059-5) / J. Ultrastruct. Res. / Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers by De Petris S. (1967)
Forsberg , C. W. , J. W. Costerton , and R. A. MacLeod . 1971 . Separation and localization of cell wall layers of a gram-negative bacterium . J. Bacteriol. 104 : 1338 - 1353 . (10.1128/jb.104.3.1338-1353.1970) / J. Bacteriol. / Separation and localization of cell wall layers of a gram-negative bacterium by Forsberg C. W. (1971)
Forsberg , C. W. , J. W. Costerton , and R. A. MacLeod . 1971 . Quantitation, chemical characteristics, and ultrastructure of the three outer cell wall layers of a gram-negative bacterium . J. Bacteriol. 104 : 1354 - 1368 . (10.1128/jb.104.3.1354-1368.1970) / J. Bacteriol. / Quantitation, chemical characteristics, and ultrastructure of the three outer cell wall layers of a gram-negative bacterium by Forsberg C. W. (1971)
Inouye , M. 1969 . Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli . J. Bacteriol. 99 : 842 - 850 . (10.1128/jb.99.3.842-850.1969) / J. Bacteriol. / Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli by Inouye M. (1969)
Inouye , M. 1971 . Internal standards for molecular weight determinations of proteins by polyacrylamide gel electrophoresis . J. Biol. Chem. 246 : 4834 - 4838 . (10.1016/S0021-9258(18)62011-3) / J. Biol. Chem. / Internal standards for molecular weight determinations of proteins by polyacrylamide gel electrophoresis by Inouye M. (1971)
Inouye , M. , and J. P. Guthrie . 1969 . A mutation which changes a membrane protein of E. coli . Proc. Nat. Acad. Sci. U.S.A. 64 : 957 - 961 . (10.1073/pnas.64.3.957) / Proc. Nat. Acad. Sci. U.S.A. / A mutation which changes a membrane protein of E. coli by Inouye M. (1969)
Inouye , M. , and A. B. Pardee . 1970 . Changes of membrane proteins and their relation to DNA synthesis and cell division of Escherichia coli . J. Biol. Chem. 245 : 5813 - 5819 . (10.1016/S0021-9258(18)62725-5) / J. Biol. Chem. / Changes of membrane proteins and their relation to DNA synthesis and cell division of Escherichia coli by Inouye M. (1970)
Martin , E. L. , and R. A. MacLeod . 1971 . Isolation and chemical composition of the cytoplasmic membrane of a gram-negative bacteria . J. Bacteriol. 105 : 1160 - 1167 . (10.1128/jb.105.3.1160-1167.1971) / J. Bacteriol. / Isolation and chemical composition of the cytoplasmic membrane of a gram-negative bacteria by Martin E. L. (1971)
Miura , T. , and S. Mizushima . 1969 . Separation and properties of outer and cytoplasmic membranes in Escherichia coli . Biochim. Biophys. Acta 193 : 268 - 276 . (10.1016/0005-2736(69)90188-6) / Biochim. Biophys. Acta / Separation and properties of outer and cytoplasmic membranes in Escherichia coli by Miura T. (1969)
Murray , R. G. E. , P. Steed , and H. H. Elson . 1965 . The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria . Can. J. Microbiol. 11 : 547 - 560 . (10.1139/m65-072) / Can. J. Microbiol. / The location of the mucopeptide in sections of the cell wall of Escherichia coli and other gram-negative bacteria by Murray R. G. E. (1965)
Schnaitman , C. A. 1970 . Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli . J. Bacteriol. 104 : 890 - 901 . (10.1128/jb.104.2.890-901.1970) / J. Bacteriol. / Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli by Schnaitman C. A. (1970)
Schnaitman , C. A. 1971 . Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X100 . J. Bacteriol. 108 : 545 - 552 . (10.1128/jb.108.1.545-552.1971) / J. Bacteriol. / Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X100 by Schnaitman C. A. (1971)
Schnaitman , C. A. 1971 . Effect of ethylenediaminetetraacetic acid, Triton X-100, and Iysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli . J. Bacteriol. 108 : 553 - 563 . (10.1128/jb.108.1.553-563.1971) / J. Bacteriol. / Effect of ethylenediaminetetraacetic acid, Triton X-100, and Iysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli by Schnaitman C. A. (1971)
Van Gool , A. P. , and N. Nanninga . 1971 . Fracture faces in the cell envelope of Escherichia coli . J. Bacteriol. 108 : 474 - 481 . (10.1128/jb.108.1.474-481.1971) / J. Bacteriol. / Fracture faces in the cell envelope of Escherichia coli by Van Gool A. P. (1971)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 9:38 a.m.)
Deposited	4 years, 2 months ago (June 13, 2021, 1:42 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:23 p.m.)
Issued	52 years, 11 months ago (Oct. 1, 1972)
Published	52 years, 11 months ago (Oct. 1, 1972)
Published Print	52 years, 11 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Inouye_1972, title={Specific Removal of Proteins from the Envelope of Escherichia coli by Protease Treatments}, volume={112}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.112.1.585-592.1972}, DOI={10.1128/jb.112.1.585-592.1972}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Inouye, Masayori and Yee, Mei-Lan}, year={1972}, month=oct, pages={585–592} }

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  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}