DOI: 10.1128/jb.112.1.17-29.1972. Deletion Mapping of the Genes Coding for HPr and Enzyme I of the Phosphoenolpyruvate: Sugar Phosphotransferase System in <i>Salmonella typhimurium</i>

Deletion Mapping of the Genes Coding for HPr and Enzyme I of the Phosphoenolpyruvate: Sugar Phosphotransferase System in Salmonella typhimurium

10.1128/jb.112.1.17-29.1972

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Sugars transported by a bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) require two soluble proteins: HPr, a low-molecular-weight phosphate-carrier protein, and enzyme I. The structural genes coding for HPr ( ptsH ) and Enzyme I ( ptsI ) are shown to be cotransducible in Salmonella typhimurium . The gene order of this region of the Salmonella chromosome is cysA-trzA-ptsH-ptsI ...( crr ). A method for the isolation of trzA-pts deletion is described. One class of pts deletions extends through ptsH and into ptsI ; a second class includes both ptsH and ptsI and extends into or through the crr gene. The crr gene either codes for or regulates the synthesis of a third PTS protein (factor III) which is sugar-specific. A hypothesis is presented for a mechanism of deletion formation.

Bibliography

Cordaro, J. C., & Roseman, S. (1972). Deletion Mapping of the Genes Coding for HPr and Enzyme I of the Phosphoenolpyruvate: Sugar Phosphotransferase System in Salmonella typhimurium. Journal of Bacteriology, 112(1), 17â29.

Authors 2

J. Christopher Cordaro (first)
Saul Roseman (additional)

References 19 Referenced 92

Anderson , B. , N. Weigel , W. Kundig , and S. Roseman . 1971 . Sugar transport. III. Purification and properties of a phospho-carrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli . J. Biol. Chem. 246 : 7023 - 7033 . / J. Biol. Chem. / Sugar transport. III. Purification and properties of a phospho-carrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli by Anderson B. (1971)
Blume , A. J. , and E. Balbinder . 1966 . The tryptophan operon of Salmonella typhimurium. Fine-structure analysis by deletion mapping and abortive transduction . Genetics 53 : 577 - 592 . (10.1093/genetics/53.3.577) / Genetics / The tryptophan operon of Salmonella typhimurium. Fine-structure analysis by deletion mapping and abortive transduction by Blume A. J. (1966)
Boro , H. , and J. E. Brenchley . 1971 . A new generalized transducing phage for Salmonella typhimurium LT2 . Virology 45 : 835 - 836 . (10.1016/0042-6822(71)90208-X) / Virology / A new generalized transducing phage for Salmonella typhimurium LT2 by Boro H. (1971)
Campbell , A. M. 1962 . Episomes . Advan. Genet. 11 : 101 - 145 . (10.1016/S0065-2660(08)60286-2) / Advan. Genet. / Episomes by Campbell A. M. (1962)
Davis , B. D. , and E. S. Mingioli . 1950 . Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bacteriol. 60 : 17 - 28 . (10.1128/jb.60.1.17-28.1950) / J. Bacteriol. / Mutants of Escherichia coli requiring methionine or vitamin B12 by Davis B. D. (1950)
Epstein , W. , S. Jewett , and C. F. Fox . 1970 . Isolation and mapping of phosphotransferase mutants in Escherichia coli . J. Bacteriol. 104 : 793 - 797 . (10.1128/jb.104.2.793-797.1970) / J. Bacteriol. / Isolation and mapping of phosphotransferase mutants in Escherichia coli by Epstein W. (1970)
Kundig , W. , S. Ghosh , and S. Roseman . 1964 . Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system . Proc. Nat. Acad. Sci. U.S.A. 52 : 1067 - 1074 . (10.1073/pnas.52.4.1067) / Proc. Nat. Acad. Sci. U.S.A. / Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system by Kundig W. (1964)
Kundig , W. , and S. Roseman . 1971 . Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli . J. Biol. Chem. 246 : 1393 - 1406 . / J. Biol. Chem. / Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli by Kundig W. (1971)
Kundig , W. , and S. Roseman . 1971 . Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the E. coli phosphotransferase system . J. Biol. Chem. 246 : 1407 - 1418 . / J. Biol. Chem. / Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the E. coli phosphotransferase system by Kundig W. (1971)
Layne E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins p. 447-454. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 3. Academic Press Inc. New York. (10.1016/S0076-6879(57)03413-8)
Levinthal , M. , and R. D. Simoni . 1969 . Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium . J. Bacteriol. 97 : 250 - 255 . (10.1128/jb.97.1.250-255.1969) / J. Bacteriol. / Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium by Levinthal M. (1969)
Ohta , N. , P. R. Galsworth , and A. B. Pardee . 1971 . Genetics of sulfate transport by Salmonella typhimurium . J. Bacteriol. 105 : 1053 - 1062 . (10.1128/jb.105.3.1053-1062.1971) / J. Bacteriol. / Genetics of sulfate transport by Salmonella typhimurium by Ohta N. (1971)
Roseman , S. 1969 . The transport of carbohydrates by a bacterial phosphotransferase system . J. Gen. Physiol. 54 : 138s - 1180 s. (10.1085/jgp.54.1.138) / J. Gen. Physiol. / The transport of carbohydrates by a bacterial phosphotransferase system by Roseman S. (1969)
Saier , M. H. , Jr. , and S. Roseman . 1972 . Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system . J. Biol. Chem. 247 : 972 - 975 . (10.1016/S0021-9258(19)45703-7) / J. Biol. Chem. / Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system by Saier M. H. (1972)
Saier , M. H. , Jr. , R D. Simoni , and S. Roseman . 1970 . The physiological behavior of enzyme I and heatstable protein mutants of a bacterial phosphotransferase system . J. Biol. Chem. 245 : 5870 - 5873 . (10.1016/S0021-9258(18)62735-8) / J. Biol. Chem. / The physiological behavior of enzyme I and heatstable protein mutants of a bacterial phosphotransferase system by Saier M. H. (1970)
Sanderson , K. 1970 . Current linkage map of Salmonella typhimurium . Bacteriol. Rev. 34 : 176 - 193 . (10.1128/br.34.2.176-193.1970) / Bacteriol. Rev. / Current linkage map of Salmonella typhimurium by Sanderson K. (1970)
Schwartz , D. O. , and J. Beckwith . 1969 . Mutagens which cause deletions in Escherichia coli . Genetics 61 : 371 - 376 . (10.1093/genetics/61.2.371) / Genetics / Mutagens which cause deletions in Escherichia coli by Schwartz D. O. (1969)
Simoni , R. D. , M. Levinthal , F. D. Kundig , W. Kundig , B. Anderson , P. E. Hartman , and S. Roseman . 1967 . Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport . Proc. Nat. Acad. Sci. U.S.A. 58 : 1963 - 1970 . (10.1073/pnas.58.5.1963) / Proc. Nat. Acad. Sci. U.S.A. / Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport by Simoni R. D. (1967)
Smith , H. O. , and M. Levine . 1967 . A phage P22 gene controlling integration of prophage . Virology 31 : 207 - 216 . (10.1016/0042-6822(67)90164-X) / Virology / A phage P22 gene controlling integration of prophage by Smith H. O. (1967)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 8:39 a.m.)
Deposited	4 years, 2 months ago (June 13, 2021, 1:33 a.m.)
Indexed	1 year, 7 months ago (Jan. 23, 2024, 6:57 p.m.)
Issued	52 years, 11 months ago (Oct. 1, 1972)
Published	52 years, 11 months ago (Oct. 1, 1972)
Published Print	52 years, 11 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Cordaro_1972, title={Deletion Mapping of the Genes Coding for HPr and Enzyme I of the Phosphoenolpyruvate: Sugar Phosphotransferase System in Salmonella typhimurium}, volume={112}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.112.1.17-29.1972}, DOI={10.1128/jb.112.1.17-29.1972}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Cordaro, J. Christopher and Roseman, Saul}, year={1972}, month=oct, pages={17–29} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        23
      ]
    ],
    "date-time": "2024-01-23T23:57:15Z",
    "timestamp": 1706054235785
  },
  "reference-count": 19,
  "publisher": "American Society for Microbiology",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1972,
            10,
            1
          ]
        ],
        "date-time": "1972-10-01T00:00:00Z",
        "timestamp": 86745600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "abstract": "<jats:p>\n            Sugars transported by a bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) require two soluble proteins: HPr, a low-molecular-weight phosphate-carrier protein, and enzyme I. The structural genes coding for HPr (\n            <jats:italic>ptsH</jats:italic>\n            ) and Enzyme I (\n            <jats:italic>ptsI</jats:italic>\n            ) are shown to be cotransducible in\n            <jats:italic>Salmonella typhimurium</jats:italic>\n            . The gene order of this region of the\n            <jats:italic>Salmonella</jats:italic>\n            chromosome is\n            <jats:italic>cysA-trzA-ptsH-ptsI</jats:italic>\n            ...(\n            <jats:italic>crr</jats:italic>\n            ). A method for the isolation of\n            <jats:italic>trzA-pts</jats:italic>\n            deletion is described. One class of\n            <jats:italic>pts</jats:italic>\n            deletions extends through\n            <jats:italic>ptsH</jats:italic>\n            and into\n            <jats:italic>ptsI</jats:italic>\n            ; a second class includes both\n            <jats:italic>ptsH</jats:italic>\n            and\n            <jats:italic>ptsI</jats:italic>\n            and extends into or through the\n            <jats:italic>crr</jats:italic>\n            gene. The\n            <jats:italic>crr</jats:italic>\n            gene either codes for or regulates the synthesis of a third PTS protein (factor III) which is sugar-specific. A hypothesis is presented for a mechanism of deletion formation.\n          </jats:p>",
  "DOI": "10.1128/jb.112.1.17-29.1972",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T13:39:25Z",
    "timestamp": 1578058765000
  },
  "page": "17-29",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 92,
  "title": "Deletion Mapping of the Genes Coding for HPr and Enzyme I of the Phosphoenolpyruvate: Sugar Phosphotransferase System in\n            <i>Salmonella typhimurium</i>",
  "prefix": "10.1128",
  "volume": "112",
  "author": [
    {
      "given": "J. Christopher",
      "family": "Cordaro",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biology and the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218"
        }
      ]
    },
    {
      "given": "Saul",
      "family": "Roseman",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biology and the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "first-page": "7023",
      "article-title": "Sugar transport. III. Purification and properties of a phospho-carrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli",
      "volume": "246",
      "author": "Anderson B.",
      "year": "1971",
      "unstructured": "Anderson , B. , N. Weigel , W. Kundig , and S. Roseman . 1971 . Sugar transport. III. Purification and properties of a phospho-carrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli . J. Biol. Chem. 246 : 7023 - 7033 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "577",
      "DOI": "10.1093/genetics/53.3.577",
      "article-title": "The tryptophan operon of Salmonella typhimurium. Fine-structure analysis by deletion mapping and abortive transduction",
      "volume": "53",
      "author": "Blume A. J.",
      "year": "1966",
      "unstructured": "Blume , A. J. , and E. Balbinder . 1966 . The tryptophan operon of Salmonella typhimurium. Fine-structure analysis by deletion mapping and abortive transduction . Genetics 53 : 577 - 592 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "835",
      "DOI": "10.1016/0042-6822(71)90208-X",
      "article-title": "A new generalized transducing phage for Salmonella typhimurium LT2",
      "volume": "45",
      "author": "Boro H.",
      "year": "1971",
      "unstructured": "Boro , H. , and J. E. Brenchley . 1971 . A new generalized transducing phage for Salmonella typhimurium LT2 . Virology 45 : 835 - 836 .",
      "journal-title": "Virology"
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "101",
      "DOI": "10.1016/S0065-2660(08)60286-2",
      "article-title": "Episomes",
      "volume": "11",
      "author": "Campbell A. M.",
      "year": "1962",
      "unstructured": "Campbell , A. M. 1962 . Episomes . Advan. Genet. 11 : 101 - 145 .",
      "journal-title": "Advan. Genet."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1128/jb.60.1.17-28.1950",
      "article-title": "Mutants of Escherichia coli requiring methionine or vitamin B12",
      "volume": "60",
      "author": "Davis B. D.",
      "year": "1950",
      "unstructured": "Davis , B. D. , and E. S. Mingioli . 1950 . Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bacteriol. 60 : 17 - 28 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "793",
      "DOI": "10.1128/jb.104.2.793-797.1970",
      "article-title": "Isolation and mapping of phosphotransferase mutants in Escherichia coli",
      "volume": "104",
      "author": "Epstein W.",
      "year": "1970",
      "unstructured": "Epstein , W. , S. Jewett , and C. F. Fox . 1970 . Isolation and mapping of phosphotransferase mutants in Escherichia coli . J. Bacteriol. 104 : 793 - 797 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "1067",
      "DOI": "10.1073/pnas.52.4.1067",
      "article-title": "Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system",
      "volume": "52",
      "author": "Kundig W.",
      "year": "1964",
      "unstructured": "Kundig , W. , S. Ghosh , and S. Roseman . 1964 . Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system . Proc. Nat. Acad. Sci. U.S.A. 52 : 1067 - 1074 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_8",
      "first-page": "1393",
      "article-title": "Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli",
      "volume": "246",
      "author": "Kundig W.",
      "year": "1971",
      "unstructured": "Kundig , W. , and S. Roseman . 1971 . Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli . J. Biol. Chem. 246 : 1393 - 1406 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_9",
      "first-page": "1407",
      "article-title": "Sugar transport. II. Characterization of constitutive membrane-bound enzymes\n                  II of the E. coli phosphotransferase system",
      "volume": "246",
      "author": "Kundig W.",
      "year": "1971",
      "unstructured": "Kundig , W. , and S. Roseman . 1971 . Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the E. coli phosphotransferase system . J. Biol. Chem. 246 : 1407 - 1418 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "unstructured": "Layne E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins p. 447-454. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 3. Academic Press Inc. New York.",
      "DOI": "10.1016/S0076-6879(57)03413-8"
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "250",
      "DOI": "10.1128/jb.97.1.250-255.1969",
      "article-title": "Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium",
      "volume": "97",
      "author": "Levinthal M.",
      "year": "1969",
      "unstructured": "Levinthal , M. , and R. D. Simoni . 1969 . Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium . J. Bacteriol. 97 : 250 - 255 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "1053",
      "DOI": "10.1128/jb.105.3.1053-1062.1971",
      "article-title": "Genetics of sulfate transport by Salmonella typhimurium",
      "volume": "105",
      "author": "Ohta N.",
      "year": "1971",
      "unstructured": "Ohta , N. , P. R. Galsworth , and A. B. Pardee . 1971 . Genetics of sulfate transport by Salmonella typhimurium . J. Bacteriol. 105 : 1053 - 1062 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "138s",
      "DOI": "10.1085/jgp.54.1.138",
      "article-title": "The transport of carbohydrates by a bacterial phosphotransferase system",
      "volume": "54",
      "author": "Roseman S.",
      "year": "1969",
      "unstructured": "Roseman , S. 1969 . The transport of carbohydrates by a bacterial phosphotransferase system . J. Gen. Physiol. 54 : 138s - 1180 s.",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "972",
      "DOI": "10.1016/S0021-9258(19)45703-7",
      "article-title": "Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system",
      "volume": "247",
      "author": "Saier M. H.",
      "year": "1972",
      "unstructured": "Saier , M. H. , Jr. , and S. Roseman . 1972 . Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system . J. Biol. Chem. 247 : 972 - 975 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "5870",
      "DOI": "10.1016/S0021-9258(18)62735-8",
      "article-title": "The physiological behavior of enzyme I and heatstable protein mutants of a bacterial phosphotransferase system",
      "volume": "245",
      "author": "Saier M. H.",
      "year": "1970",
      "unstructured": "Saier , M. H. , Jr. , R D. Simoni , and S. Roseman . 1970 . The physiological behavior of enzyme I and heatstable protein mutants of a bacterial phosphotransferase system . J. Biol. Chem. 245 : 5870 - 5873 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_16",
      "doi-asserted-by": "crossref",
      "first-page": "176",
      "DOI": "10.1128/br.34.2.176-193.1970",
      "article-title": "Current linkage map of Salmonella typhimurium",
      "volume": "34",
      "author": "Sanderson K.",
      "year": "1970",
      "unstructured": "Sanderson , K. 1970 . Current linkage map of Salmonella typhimurium . Bacteriol. Rev. 34 : 176 - 193 .",
      "journal-title": "Bacteriol. Rev."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "371",
      "DOI": "10.1093/genetics/61.2.371",
      "article-title": "Mutagens which cause deletions in Escherichia coli",
      "volume": "61",
      "author": "Schwartz D. O.",
      "year": "1969",
      "unstructured": "Schwartz , D. O. , and J. Beckwith . 1969 . Mutagens which cause deletions in Escherichia coli . Genetics 61 : 371 - 376 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "1963",
      "DOI": "10.1073/pnas.58.5.1963",
      "article-title": "Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport",
      "volume": "58",
      "author": "Simoni R. D.",
      "year": "1967",
      "unstructured": "Simoni , R. D. , M. Levinthal , F. D. Kundig , W. Kundig , B. Anderson , P. E. Hartman , and S. Roseman . 1967 . Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport . Proc. Nat. Acad. Sci. U.S.A. 58 : 1963 - 1970 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1016/0042-6822(67)90164-X",
      "article-title": "A phage P22 gene controlling integration of prophage",
      "volume": "31",
      "author": "Smith H. O.",
      "year": "1967",
      "unstructured": "Smith , H. O. , and M. Levine . 1967 . A phage P22 gene controlling integration of prophage . Virology 31 : 207 - 216 .",
      "journal-title": "Virology"
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.112.1.17-29.1972",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.112.1.17-29.1972",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        6,
        13
      ]
    ],
    "date-time": "2021-06-13T05:33:30Z",
    "timestamp": 1623562410000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.112.1.17-29.1972"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "references-count": 19,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1972,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.112.1.17-29.1972"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.112.1.17-29.1972",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}