DOI: 10.1128/jb.109.2.499-504.1972. Transport of <i>S</i> -Adenosylmethionine in <i>Saccharomyces cerevisiae</i>

Transport of S -Adenosylmethionine in Saccharomyces cerevisiae

10.1128/jb.109.2.499-504.1972

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The properties of a specific system for the transport of S -adenosylmethionine in yeast are described. The process was p H-, temperature-, and energy-dependent, and showed saturation kinetics. The K m for the system was 3.3 × 10 −6 m . Of the S -adenosylmethionine moieties tested, only S -adenosylhomocysteine competitively inhibited the uptake of the adenosylsulfonium compound. Adenine, adenosine, methionine, homocysteine, and the sulfonium compound S -methylmethionine were without effect. The analogue S -adenosylethionine showed competitive inhibition. Under conditions of inhibition of protein synthesis by cycloheximide or methionine starvation, permease activity was stable. The mutant sam-p3 apparently was able to transport S -adenosylmethionine only by diffusion. Uptake by diploids containing this mutation was directly proportional to the gene dose.

Bibliography

Murphy, J. T., & Spence, K. D. (1972). Transport of S -Adenosylmethionine in Saccharomyces cerevisiae. Journal of Bacteriology, 109(2), 499â504.

Authors 2

J. T. Murphy (first)
K. D. Spence (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 8:38 a.m.)
Deposited	4 years ago (July 29, 2021, 1:01 p.m.)
Indexed	1 year, 10 months ago (Oct. 8, 2023, 4:50 a.m.)
Issued	53 years, 6 months ago (Feb. 1, 1972)
Published	53 years, 6 months ago (Feb. 1, 1972)
Published Print	53 years, 6 months ago (Feb. 1, 1972)

Funders 0

None

BibTeX

@article{Murphy_1972, title={Transport of S -Adenosylmethionine in Saccharomyces cerevisiae}, volume={109}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.109.2.499-504.1972}, DOI={10.1128/jb.109.2.499-504.1972}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Murphy, J. T. and Spence, K. D.}, year={1972}, month=feb, pages={499–504} }

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