Separation and Localization of Cell Wall Layers of a Gram-Negative Bacterium
10.1128/jb.104.3.1338-1353.1970
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The cell envelope of a marine pseudomonad as seen in thin section by electron microscopy has the double-membrane structure typical of other gram-negative bacteria. Cells washed with a solution containing Na + , K + , and Mg ++ at their concentrations in the growth medium, when suspended briefly in 0.5 m sucrose, lost 13% of their hexosamine in a form nonsedimentable by centrifugation at 73,000 × g . Since the resulting cells in thin section appeared unchanged, it was concluded that the material released was derived from a nonstaining, loosely bound outer layer. This same layer could be removed from the cells by washing with 0.5 m NaCl. A second nonsedimentable fraction was released after successive suspension of the cells in 0.5 m sucrose. Since this material was released only when the outer double-track structure had broken, it was concluded that it arose from a layer immediately underlying the latter layer. The three layers differed in their content of hexosamine and protein. None of the layers released contained muramic or diaminopimelic acid. The cell form remaining was rod shaped and appeared in thin section to be bounded only by its cytoplasmic membrane. This form contained all the muramic and diaminopimelic acid in the cell. Treatment with lysozyme released the muramic and diaminopimelic acid and converted the rod form to a protoplast, indicating that in the rod form (mureinoplast) a thin layer of peptidoglycan is located on the outside surface of the cytoplasmic membrane. Thus, five separate layers have been detected in the cell envelope of this marine pseudomonad.

Bibliography

Forsberg, C. W., Costerton, J. W., & MacLeod, R. A. (1970). Separation and Localization of Cell Wall Layers of a Gram-Negative Bacterium. Journal of Bacteriology, 104(3), 1338–1353.

Authors 3
  1. C. W. Forsberg (first)
  2. J. W. Costerton (additional)
  3. Robert A. MacLeod (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 3, 2020, 9:28 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 12:56 p.m.)
Indexed 1 year, 2 months ago (June 30, 2024, 5:07 p.m.)
Issued 54 years, 9 months ago (Dec. 1, 1970)
Published 54 years, 9 months ago (Dec. 1, 1970)
Published Print 54 years, 9 months ago (Dec. 1, 1970)
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@article{Forsberg_1970, title={Separation and Localization of Cell Wall Layers of a Gram-Negative Bacterium}, volume={104}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.104.3.1338-1353.1970}, DOI={10.1128/jb.104.3.1338-1353.1970}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Forsberg, C. W. and Costerton, J. W. and MacLeod, Robert A.}, year={1970}, month=dec, pages={1338–1353} }