In order to relate the biogenesis of the lactose transport system to lipid synthesis, a glycerol-requiring mutant of Escherichia coli K-12 with a specific defect in l -glycerol-3-phosphate synthesis was isolated and characterized. The defective enzyme is the biosynthetic l -glycerol-3-phosphate dehydrogenase [ l -glycerol-3-phosphate: NAD (P) oxidoreductase, EC 1.1.1.8] which functions as a dihydroxyacetone phosphate reductase to provide l -glycerol-3-phosphate for lipid synthesis. In this mutant, removal of glycerol from the growth medium results in inhibition of the synthesis of protein, deoxyribonucleic acid, and phospholipid. Inhibition of phospholipid synthesis immediately follows glycerol removal, whereas the inhibition of deoxyribonucleic acid and protein synthesis is preceded by a short lag period. Glycerol starvation does not change the turnover pattern of previously synthesized phospholipids. The blocking of lipid synthesis by glycerol starvation causes a drastic decrease in inducibility of β-galactoside transport activity relative to β-galactosidase, indicating that induction of lactose transport requires de novo lipid synthesis.

Hsu, C. C., & Fox, C. F. (1970). Induction of the Lactose Transport System in a Lipid-Synthesis-Defective Mutant of Escherichia coli. Journal of Bacteriology, 103(2), 410–416.