Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties
10.1128/jb.102.1.178-186.1970
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

An endonuclease purified ∼3,200-fold from Micrococcus luteus is active on native ultraviolet-irradiated deoxyribonucleic acid (DNA), but is inactive on unirradiated native or denatured DNA and has no activity toward irradiated denatured DNA. The major type of lesion for the nucleolytic activity is the cyclobutane pyrimidine dimer. The enzyme makes a number of single-strand breaks approximately equal to the number of dimers, but dimers are not excised. This endonuclease—a small molecular weight protein—therefore has all the attributes hypothesized for the first enzyme in the sequential steps in repair of DNA in vivo. Another paper shows that the endonuclease is able to reactivate ultraviolet-irradiated transforming DNA.

Bibliography

Carrier, W. L., & Setlow, R. B. (1970). Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties. Journal of Bacteriology, 102(1), 178–186.

Authors 2
  1. W. L. Carrier (first)
  2. R. B. Setlow (additional)
References 32 Referenced 192
  1. Bodmer , W. F. , and A. T. Ganesan . 1964 . Biochemical and genetic studies of integration and recombination in Bacillus subtilis transformation . Genetics 50 : 717 - 738 . (10.1093/genetics/50.4.717) / Genetics / Biochemical and genetic studies of integration and recombination in Bacillus subtilis transformation by Bodmer W. F. (1964)
  2. Carrier , W. L. , and R. B. Setlow . 1966 . Excision of pyrimidine dimers from irradiated deoxyribonucleic acid in vitro . Biochim. Biophys. Acta 129 : 318 - 325 . (10.1016/0005-2787(66)90374-1) / Biochim. Biophys. Acta / Excision of pyrimidine dimers from irradiated deoxyribonucleic acid in vitro by Carrier W. L. (1966)
  3. Elder , R. L. , and R. F. Beers , Jr. 1965 . Nonphotoreactivating repair of ultraviolet light-damaged transforming deoxyribonucleic acid by Micrococcus Iysodeikticus extracts . J. Bacteriol. 90 : 681 - 686 . (10.1128/JB.90.3.681-686.1965) / J. Bacteriol. / Nonphotoreactivating repair of ultraviolet light-damaged transforming deoxyribonucleic acid by Micrococcus Iysodeikticus extracts by Elder R. L. (1965)
  4. Grossman L. 1967. The preparation of phosphorus-32 labeled DNA p. 700-702. In L. Grossman and K. Moldave (ed.) Methods in enzymology vol. XII part A. Academic Press Inc. New York. (10.1016/S0076-6879(67)12107-1)
  5. Grossman , L. , J. C. Kaplan , S. R. Kushner , and I. Mahler . 1969 . Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA . Cold Spring Harbor Symp. Quant. Biol. 33 : 229 - 234 . (10.1101/SQB.1968.033.01.026) / Cold Spring Harbor Symp. Quant. Biol. / Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA by Grossman L. (1969)
  6. Hanawalt P. C. 1968. Cellular recovery from photochemical damage p. 203-251. In A. C. Giese (ed.) Photophysiology vol. IV. Academic Press Inc. New York. (10.1016/B978-1-4832-2781-8.50010-8)
  7. Haynes R. H. R. M. Baker and G. E. Jones. 1968. Genetic implications of DNA repair p. 425-465. In G. 0. Phillips (ed.) Energetics and mechanisms in radiation biology. Academic Press Inc. London.
  8. Hirs , C. H. W. , S. Moore , and W. H. Stein . 1955 . Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuciease . J. Biol. Chem. 219 : 623 - 642 . (10.1016/S0021-9258(18)65722-9) / J. Biol. Chem. / Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuciease by Hirs C. H. W. (1955)
  9. Howard-Flanders , P. 1968 . DNA repair. Annu . Rev. Biochem. 37 : 175 - 200 . (10.1146/annurev.bi.37.070168.001135) / Rev. Biochem. / DNA repair. Annu by Howard-Flanders P. (1968)
  10. Kaplan , J. C. , S. R. Kushner , and L. Grossman . 1969 . Enzymatic repair of DNA. I. Purification of two enzymes involved in the excision of thymine dimers from ultravioletirradiated DNA . Proc. Nat. Acad. Sci. U.S.A. 63 : 144 - 151 . (10.1073/pnas.63.1.144) / Proc. Nat. Acad. Sci. U.S.A. / Enzymatic repair of DNA. I. Purification of two enzymes involved in the excision of thymine dimers from ultravioletirradiated DNA by Kaplan J. C. (1969)
  11. Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
  12. Marmur , J. 1961 . A procedure for the isolation of deoxyribonucleic acid from micro-organisms . J. Mol. Biol. 3 : 208 - 218 . (10.1016/S0022-2836(61)80047-8) / J. Mol. Biol. / A procedure for the isolation of deoxyribonucleic acid from micro-organisms by Marmur J. (1961)
  13. Moriguchi , E. , and K. Suzuki . 1966 . Enzymatic breakdown of UV-irradiated DNA by the extract from Micrococcus lysodeikticus . Biochem. Biophys. Res. Commun. 24 : 195 - 202 . (10.1016/0006-291X(66)90719-4) / Biochem. Biophys. Res. Commun. / Enzymatic breakdown of UV-irradiated DNA by the extract from Micrococcus lysodeikticus by Moriguchi E. (1966)
  14. Muhammed , A. 1966 . Studies on the yeast photoreactivating enzyme. I. A method for the large scale purification and some properties of the enzyme . J. Biol. Chem. 241 : 516 - 523 . / J. Biol. Chem. / Studies on the yeast photoreactivating enzyme. I. A method for the large scale purification and some properties of the enzyme by Muhammed A. (1966)
  15. Nakayama , H. , S. Okubo , M. Sekiguchi , and Y. Takagi . 1967 . A deoxyribonuclease activity specific for ultravioletirradiated DNA: a chromatographic analysis . Biochem. Biophys. Res. Commun. 27 : 217 - 223 . (10.1016/S0006-291X(67)80064-0) / Biochem. Biophys. Res. Commun. / A deoxyribonuclease activity specific for ultravioletirradiated DNA: a chromatographic analysis by Nakayama H. (1967)
  16. Notani , N. , and S. H. Goodgal . 1966 . On the nature of recombinants formed during transformation in Haemophilus influenzae . J. Gen. Physiol. 49 : 197 - 208 . (10.1085/jgp.49.6.197) / J. Gen. Physiol. / On the nature of recombinants formed during transformation in Haemophilus influenzae by Notani N. (1966)
  17. Okubo , S. , H. Nakayama , M. Sekiguchi , and Y. Takagi . 1967 . A mutant of Micrococcus Iysodeikticus defective in a deoxyribonuclease activity specific for ultraviolet-irradiated DNA . Biochem. Biophys. Res. Commun. , 27 : 224 - 229 . (10.1016/S0006-291X(67)80065-2) / Biochem. Biophys. Res. Commun. / A mutant of Micrococcus Iysodeikticus defective in a deoxyribonuclease activity specific for ultraviolet-irradiated DNA by Okubo S. (1967)
  18. Rorsch , A. , C. van der Kamp , and J. Adema . 1964 . Dark reactivation of ultraviolet-irradiated bacteriophage deoxyribonucleic acid in vitro . Biochim. Biophys. Acta 80 : 346 - 348 . / Biochim. Biophys. Acta / Dark reactivation of ultraviolet-irradiated bacteriophage deoxyribonucleic acid in vitro by Rorsch A. (1964)
  19. Rupert , C. S. , and W. Harm . 1966 . Reactivation after photobiological damage . Advan. Radiat. Biol. 2 : 1 - 81 . (10.1016/B978-1-4832-3121-1.50006-2) / Advan. Radiat. Biol. / Reactivation after photobiological damage by Rupert C. S. (1966)
  20. Setlow J. K. 1967. The effects of ultraviolet radiation and photoreactivation p. 157-209. In M. Florkin and E. H. Statz (ed.) Comprehensive biochemistry vol. 27. Elsevier Amsterdam. (10.1016/B978-1-4831-9716-6.50013-6)
  21. Setlow , J. K. , M. E. Boling , and F. J. Bollum . 1965 . The chemical nature of photoreactivable lesions in DNA . Proc. Nat. Acad. Sci. U.S.A. 53 : 1430 - 1436 . (10.1073/pnas.53.6.1430) / Proc. Nat. Acad. Sci. U.S.A. / The chemical nature of photoreactivable lesions in DNA by Setlow J. K. (1965)
  22. Setlow , R. B. 1966 . Cyclobutane-type pyrimnidine dimers in polynucleotides . Science 153 : 379 - 386 . (10.1126/science.153.3734.379) / Science / Cyclobutane-type pyrimnidine dimers in polynucleotides by Setlow R. B. (1966)
  23. Setlow R. B. 1968. The photochemistry photobiology and repair of polynucleotides p. 257-295. In J. N. Davidson and W. E. Cohn (ed.) Progress in nucleic acid research and molecular biology vol. 8. Academic Press Inc. New York. (10.1016/S0079-6603(08)60548-6)
  24. Setlow , R. B. , and W. L. Carrier . 1966 . Pyrimidine dimers in ultraviolet-irradiated DNA's . J. Mol. Biol. 17 : 237 - 254 . (10.1016/S0022-2836(66)80105-5) / J. Mol. Biol. / Pyrimidine dimers in ultraviolet-irradiated DNA's by Setlow R. B. (1966)
  25. Setlow R. B. and W. L. Carrier. 1968. The excision of pyrimidine dimers in vivo and in vitro p. 134-141. In W. J. Peacock and R. D. Brock (ed.) Replication and recombination of genetic material. Australian Academy of Science Canberra.
  26. Setlow , R. B. , W. L. Carrier , and J. K. Setlow . 1969 . The action of an endonuclease specific for damaged DNA . Biophys. J. 9:A-57. / Biophys. J. 9:A-57. / The action of an endonuclease specific for damaged DNA by Setlow R. B. (1969)
  27. Shimada , K. , H. Nakayama , S. Okubo , M. Sekiguchi , and Y. Takagi . 1967 . An endonucleolytic activity specific for ultraviolet-irradiated DNA in wild type and mutant strains of Micrococcus Iysodeikticus . Biochem. Biophys. Res. Commun. 27 : 539 - 545 . (10.1016/S0006-291X(67)80021-4) / Biochem. Biophys. Res. Commun. / An endonucleolytic activity specific for ultraviolet-irradiated DNA in wild type and mutant strains of Micrococcus Iysodeikticus by Shimada K. (1967)
  28. Spies J. R. 1957. Colorimetric procedur.es for amino acids p. 468-471. In S. P. Colowick and N. 0 Kaplan (ed.) Methods in enzymology vol. III. Academic Press Inc. New York.
  29. Strauss , B. S. 1962 . Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme . Proc. Nat. Acad. Sci. U.S.A. 48 : 1670 - 1675 . (10.1073/pnas.48.9.1670) / Proc. Nat. Acad. Sci. U.S.A. / Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme by Strauss B. S. (1962)
  30. Strauss B. S. 1969. DNA repair mechanisms and their relation to mutation and recombination p. 1-85. In Current topics in microbiology and immunology vol. 44. Springer-Verlag New York.
  31. Strauss , B. , T. Searashi , and M. Robbins . 1966 . Repair of DNA studied with a nuclease specific for UV-induced lesions . Proc. Nat. Acad. Sci. U.S.A. 56 : 932 - 939 . (10.1073/pnas.56.3.932) / Proc. Nat. Acad. Sci. U.S.A. / Repair of DNA studied with a nuclease specific for UV-induced lesions by Strauss B. (1966)
  32. Takagi , Y. , M. Sekiguchi , S. Okubo , H. Nakayama , K. Shimada , S. Yasuda , T. Nishimoto , and H. Yoshihara . 1968 . Nucleases specific for ultraviolet light-irradiated DNA and their possible role in dark repair . Cold Spring Harbor Symp. Quant. Biol. 33 : 219 - 227 . (10.1101/SQB.1968.033.01.025) / Cold Spring Harbor Symp. Quant. Biol. / Nucleases specific for ultraviolet light-irradiated DNA and their possible role in dark repair by Takagi Y. (1968)
Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 8:35 a.m.)
Deposited 4 years ago (July 29, 2021, 12:55 p.m.)
Indexed 4 months, 2 weeks ago (April 11, 2025, 3:50 a.m.)
Issued 55 years, 4 months ago (April 1, 1970)
Published 55 years, 4 months ago (April 1, 1970)
Published Print 55 years, 4 months ago (April 1, 1970)
Funders 0

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@article{Carrier_1970, title={Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties}, volume={102}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.102.1.178-186.1970}, DOI={10.1128/jb.102.1.178-186.1970}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Carrier, W. L. and Setlow, R. B.}, year={1970}, month=apr, pages={178–186} }