Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
10.1128/jb.101.3.738-754.1970
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (ρ) or midpoint of thermal denaturation (T m ) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (T m – 69.4). T m appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (–1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between ρ and T m was calculated from data of 197 strains; it agrees excellently with the above two equations.

Bibliography

De Ley, J. (1970). Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid. Journal of Bacteriology, 101(3), 738–754.

Authors 1
  1. J. De Ley (first)
References 107 Referenced 354
  1. Ahrens , R. , and G. Rheinheimer . 1967 . Ueber einige sternbildende Bakterien aus der Ostsee . Kieler Meeresforsch. 23 : 127 - 136 . / Kieler Meeresforsch. / Ueber einige sternbildende Bakterien aus der Ostsee by Ahrens R. (1967)
  2. Anderson , J. R. , D. Pramer , and F. F. Davis . 1965 . Nucleic acid composition of Nitrosomonas europaea . Biochim. Biophys. Acta 108 : 155 - 157 . (10.1016/0005-2787(65)90124-3) / Biochim. Biophys. Acta / Nucleic acid composition of Nitrosomonas europaea by Anderson J. R. (1965)
  3. Auletta , A. E. , and E. R. Kennedy . 1966 . Deoxyribonucleic acid base composition of some members of the Micrococcaceae . J. Bacteriol. 92 : 28 - 34 . (10.1128/jb.92.1.28-34.1966) / J. Bacteriol. / Deoxyribonucleic acid base composition of some members of the Micrococcaceae by Auletta A. E. (1966)
  4. Belozersky A. and A. S. Spirin. 1960. Chemistry of the nucleic acids of micro-organisms p. 147-185. In E. Chargaff and J. N. Davidson (ed.) The nucleic acids vol. 3. Academic Press Inc. New York. (10.1016/B978-0-12-395718-4.50010-X)
  5. Berns , K. I. , and C. A. Thomas . 1965 . Isolation of high molecular weight DNA from Hemophilus influenzae . J. Mol. Biol. 11 : 476 - 490 . (10.1016/S0022-2836(65)80004-3) / J. Mol. Biol. / Isolation of high molecular weight DNA from Hemophilus influenzae by Berns K. I. (1965)
  6. Bovre , K. 1967 . Transformation and DNA base composition in taxonomy, with special reference to recent studies in Moraxella and Neisseria . Acta Pathol. Microbiol. Scand. 69 : 123 - 144 . (10.1111/j.1699-0463.1967.tb05132.x) / Acta Pathol. Microbiol. Scand. / Transformation and DNA base composition in taxonomy, with special reference to recent studies in Moraxella and Neisseria by Bovre K. (1967)
  7. Catlin , B. W. , and L. S. Cunningham . 1958 . Studies of extracellular and intracellular bacterial deoxyribonucleic acids . J. Gen. Microbiol. 19 : 522 - 539 . (10.1099/00221287-19-3-522) / J. Gen. Microbiol. / Studies of extracellular and intracellular bacterial deoxyribonucleic acids by Catlin B. W. (1958)
  8. Catlin , B. W. , and L. S. Cunningham . 1961 . Transforming activities and base contents of deoxyribonucleate preparations from various neisseriae . J. Gen. Microbiol. 26 : 303 - 312 . (10.1099/00221287-26-2-303) / J. Gen. Microbiol. / Transforming activities and base contents of deoxyribonucleate preparations from various neisseriae by Catlin B. W. (1961)
  9. Catlin , B. W. , and L. S. Cunningham . 1964 . Genetic transformation of Neisseria catarrhalis by deoxyribonucleate preparations having different average base composition . J. Gen. Microbiol. 37 : 341 - 352 . (10.1099/00221287-37-3-341) / J. Gen. Microbiol. / Genetic transformation of Neisseria catarrhalis by deoxyribonucleate preparations having different average base composition by Catlin B. W. (1964)
  10. Catlin , B. W. , and L. S. Cunningham . 1964 . Transforming activities and base composition of deoxyribonucleates from strains of Moraxella and Mima . J. Gen. Microbiol. 37 : 353 - 367 . (10.1099/00221287-37-3-353) / J. Gen. Microbiol. / Transforming activities and base composition of deoxyribonucleates from strains of Moraxella and Mima by Catlin B. W. (1964)
  11. Chargaff E. 1955. In E. Chargaff and J. N. Davidson (ed.) The nucleic acids vol. 1 p. 307-371. Academic Press Inc. New York.
  12. Chargaff , E. , H. M. Schulman , and H. S. Shapiro . 1957 . Protoplasts of E. coli as sources and acceptors of deoxypentose nucleic acids: rehabilitation of a deficient mutant . Nature 180 : 851 - 852 . (10.1038/180851a0) / Nature / Protoplasts of E. coli as sources and acceptors of deoxypentose nucleic acids: rehabilitation of a deficient mutant by Chargaff E. (1957)
  13. Chun , E. H. L. , M. H. Vaughan , and A. Rich . 1963 . The isolation and characterization of DNA associated with chloroplast preparations . J. Mol. Biol. 7 : 130 - 141 . (10.1016/S0022-2836(63)80042-X) / J. Mol. Biol. / The isolation and characterization of DNA associated with chloroplast preparations by Chun E. H. L. (1963)
  14. Citarella , R. V. , and R. R. Colwell . 1966 . DNA base composition of Achromobacter liquefaciens (Tulecke et al.) . Can. J. Microbiol. 12 : 418 - 420 . (10.1139/m66-058) / Can. J. Microbiol. / DNA base composition of Achromobacter liquefaciens (Tulecke et al.) by Citarella R. V. (1966)
  15. Colwell , R. R. , V. I. Adeyemo , and H. H. Kirtland . 1968 . Esterases and DNA base composition analysis of Vibrio cholerae and related vibrios . J. Appl. Bacteriol. 31 : 323 - 335 . (10.1111/j.1365-2672.1968.tb00374.x) / J. Appl. Bacteriol. / Esterases and DNA base composition analysis of Vibrio cholerae and related vibrios by Colwell R. R. (1968)
  16. Colwell , R. R. , R. V. Citarella , and P. K. Chen . 1966 . DNA base composition of Cytophaga marinoflava n. sp. determined by buoyant density measurements in CsCI . Can. J. Microbiol. 12 : 1099 - 1103 . (10.1139/m66-150) / Can. J. Microbiol. / DNA base composition of Cytophaga marinoflava n. sp. determined by buoyant density measurements in CsCI by Colwell R. R. (1966)
  17. Colwell , R. R. , R. V. Citarella , and I. Ryman . 1965 . Deoxyribonucleic acid base composition and adansonian analysis of heterotrophic, aerobic pseudomonads . J. Bacteriol. 90 : 1148 - 1149 . (10.1128/jb.90.4.1148-1149.1965) / J. Bacteriol. / Deoxyribonucleic acid base composition and adansonian analysis of heterotrophic, aerobic pseudomonads by Colwell R. R. (1965)
  18. Colwell , R. R. , and M. Mandel . 1964 . Adansonian analysis and deoxyribonucleic acid base composition of some gramnegative bacteria . J. Bacteriol. 87 : 1412 - 1422 . (10.1128/jb.87.6.1412-1422.1964) / J. Bacteriol. / Adansonian analysis and deoxyribonucleic acid base composition of some gramnegative bacteria by Colwell R. R. (1964)
  19. Colwell , R. R. , and M. Mandel . 1964 . Base composition ot deoxyribonucleic acid of marine and nonmarine vibrios deduced from buoyant-density measurements in cesium chloride . J. Bacteriol. 88 : 1816 - 1817 . (10.1128/jb.88.6.1816-1817.1964) / J. Bacteriol. / Base composition ot deoxyribonucleic acid of marine and nonmarine vibrios deduced from buoyant-density measurements in cesium chloride by Colwell R. R. (1964)
  20. Colwell , R. R. , and M. Mandel . 1965 . Adansonian analysis and deoxyribonucleic acid base composition of Serratia marcescens . J. Bacteriol. 89 : 454 - 461 . (10.1128/jb.89.2.454-461.1965) / J. Bacteriol. / Adansonian analysis and deoxyribonucleic acid base composition of Serratia marcescens by Colwell R. R. (1965)
  21. Crawford , L. V. 1963 . The physical characteristics of polyoma virus . Virology 19 : 279 - 282 . (10.1016/0042-6822(63)90065-5) / Virology / The physical characteristics of polyoma virus by Crawford L. V. (1963)
  22. Creaser , E. H. , and A. Taussig . 1957 . The purification and chromatography of bacteriophages on anion-exchange cellulose . Virology 4 : 200 - 208 . (10.1016/0042-6822(57)90057-0) / Virology / The purification and chromatography of bacteriophages on anion-exchange cellulose by Creaser E. H. (1957)
  23. Crothers , D. M. , N. R. Kallenbach , and B. H. Zimm . 1965 . The melting transition of low-molecular weight DNA: theory and experiment . J. Mol. Biol. 11 : 802 - 820 . (10.1016/S0022-2836(65)80037-7) / J. Mol. Biol. / The melting transition of low-molecular weight DNA: theory and experiment by Crothers D. M. (1965)
  24. De Ley , J. 1964 . Effect of mutation on DNA composition of some bacteria . Antonie Van Leeuwenhoek J. Microbiol. Serol. 30 : 281 - 288 . (10.1007/BF02046734) / Antonie Van Leeuwenhoek J. Microbiol. Serol. / Effect of mutation on DNA composition of some bacteria by De Ley J. (1964)
  25. De Ley , J. , M. Bernaerts , A. Rassel , and J. Guilmot . 1966 . Approach to an improved taxonomy of the genus Agrobacterium . J. Gen. Microbiol. 43 : 7 - 17 . (10.1099/00221287-43-1-7) / J. Gen. Microbiol. / Approach to an improved taxonomy of the genus Agrobacterium by De Ley J. (1966)
  26. De Ley , J. , and S. Friedman . 1964 . Deoxyribonucleic acid hybrids of acetic acid bacteria . J. Bacteriol. 88 : 937 - 945 . (10.1128/jb.88.4.937-945.1964) / J. Bacteriol. / Deoxyribonucleic acid hybrids of acetic acid bacteria by De Ley J. (1964)
  27. De Ley , J. , I. W. Park , R. Tijtgat , and J. Van Ermengem . 1966 . DNA homology and taxonomy of Pseudomonas and Xanthomonas . J. Gen. Microbiol. 42 : 43 - 56 . (10.1099/00221287-42-1-43) / J. Gen. Microbiol. / DNA homology and taxonomy of Pseudomonas and Xanthomonas by De Ley J. (1966)
  28. De Ley , J. , and J. Schell . 1963 . Deoxyribonucleic acid base composition of acetic acid bacteria . J. Gen. Microbiol. 33 : 243 - 253 . (10.1099/00221287-33-2-243) / J. Gen. Microbiol. / Deoxyribonucleic acid base composition of acetic acid bacteria by De Ley J. (1963)
  29. De Ley , J. , and J. Van Muylem . 1963 . Some applications of deoxyribonucleic acid base composition in bacterial taxonomy . Antonie Van Leeuwenhoek J. Microbiol. 29 : 344 - 358 . (10.1007/BF02046087) / Antonie Van Leeuwenhoek J. Microbiol. / Some applications of deoxyribonucleic acid base composition in bacterial taxonomy by De Ley J. (1963)
  30. Dunn , D. B. , and J. D. Smith . 1958 . The occurrence of 6-methylaminopurine in deoxyribonucleic acid . Biochem. J. 68 : 627 - 636 . (10.1042/bj0680627) / Biochem. J. / The occurrence of 6-methylaminopurine in deoxyribonucleic acid by Dunn D. B. (1958)
  31. Edelman , M. , D. Swinton , J. A. Schiff , H. T. Epstein , and B. Zeldin . 1967 . Deoxyribonucleic acid of the blue-green algae (Cyanophyta) . Bacteriol. Rev. 31 : 315 - 331 . (10.1128/br.31.4.315-331.1967) / Bacteriol. Rev. / Deoxyribonucleic acid of the blue-green algae (Cyanophyta) by Edelman M. (1967)
  32. Friedman , S. , and J. De Ley . 1965 . " Genetic species " concept in Xanthomonas. J. Bacteriol. 89 : 95 - 100 . / concept in Xanthomonas. J. Bacteriol. / Genetic species by Friedman S. (1965)
  33. Frontali , C. , L. R. Hill , and L. G. Silvestri . 1965 . The base composition of deoxyribonucleic acids of Streptomyces . J. Gen. Microbiol. 38 : 243 - 250 . (10.1099/00221287-38-2-243) / J. Gen. Microbiol. / The base composition of deoxyribonucleic acids of Streptomyces by Frontali C. (1965)
  34. Gandelman , B. , S. Zamenhof , and E. Chargaff . 1952 . The deoxypentose nucleic acids of three strains of Escherichia coli . Biochim. Biophys. Acta 9 : 399 - 401 . (10.1016/0006-3002(52)90183-2) / Biochim. Biophys. Acta / The deoxypentose nucleic acids of three strains of Escherichia coli by Gandelman B. (1952)
  35. Gasser , F. , and M. Mandel . 1968 . Deoxyribonucleic acid base composition of the genus Lactobacillus . J. Bacteriol. 96 : 580 - 588 . (10.1128/jb.96.3.580-588.1968) / J. Bacteriol. / Deoxyribonucleic acid base composition of the genus Lactobacillus by Gasser F. (1968)
  36. Gasser , F. , and M. Sebald . 1966 . Composition en bases nucleiques des bacteries du genre Lactobacillus. Ann. Inst. Pasteur 110 : 261 - 275 . / Lactobacillus. Ann. Inst. Pasteur / Composition en bases nucleiques des bacteries du genre by Gasser F. (1966)
  37. Gause , G. F. , Y. D. Dudnik , A. V. Laiko , and E. M. Netyksa . 1967 . Induction of mutants with altered DNA composition: effect of ultraviolet on Bacterium paracoli 5099 . Science 157 : 1196 - 1197 . (10.1126/science.157.3793.1196) / Science / Induction of mutants with altered DNA composition: effect of ultraviolet on Bacterium paracoli 5099 by Gause G. F. (1967)
  38. Gause , G. G. , N. P. Loshkareva , 1. B. Zbarsky , and G. F. Gause . 1964 . Deoxyribonucleic acid base composition in certain bacteria and their mutants with impaired respiration . Nature 203 : 598 - 599 . (10.1038/203598a0) / Nature / Deoxyribonucleic acid base composition in certain bacteria and their mutants with impaired respiration by Gause G. G. (1964)
  39. Green , M. 1962 . Studies on the biosynthesis of viral DNA . Cold Spring Harbor Symp. Quant. Biol. 27 : 219 - 233 . (10.1101/SQB.1962.027.001.022) / Cold Spring Harbor Symp. Quant. Biol. / Studies on the biosynthesis of viral DNA by Green M. (1962)
  40. Green , B. R. , and M. P. Gordon . 1967 . The satellite DNA's of some higher plants . Biochim. Biophys. Acta 145 : 378 - 390 . (10.1016/0005-2787(67)90056-1) / Biochim. Biophys. Acta / The satellite DNA's of some higher plants by Green B. R. (1967)
  41. Green , M. , and M. Pifia . 1963 . Biochemical studies on adenovirus multiplication . IV. Virology 20 : 199 - 207 . (10.1016/0042-6822(63)90157-0) / IV. Virology / Biochemical studies on adenovirus multiplication by Green M. (1963)
  42. Hoyer , B. H. , and N. B. Mc Cullough . 1968 . Polynucleotide homologies of Brucella deoxyribonucleic acids . J. Bacteriol. 95 : 444 - 448 . (10.1128/jb.95.2.444-448.1968) / J. Bacteriol. / Polynucleotide homologies of Brucella deoxyribonucleic acids by Hoyer B. H. (1968)
  43. Ikeda , Y. , H. Saito , K. Miura , J. Takagi , and H. Aoki . 1965 . DNA base composition, susceptibility to bacteriophages and interspecific transformation as criteria for classification in the genus Bacillus . J. Gen. Appl. Microbiol. 11 : 181 - 190 . (10.2323/jgam.11.181) / J. Gen. Appl. Microbiol. / DNA base composition, susceptibility to bacteriophages and interspecific transformation as criteria for classification in the genus Bacillus by Ikeda Y. (1965)
  44. Inman , R. B. 1964 . Transitions of DNA homopolymers . J. Mol. Biol. 9 : 624 - 637 . (10.1016/S0022-2836(64)80171-6) / J. Mol. Biol. / Transitions of DNA homopolymers by Inman R. B. (1964)
  45. Inman , R. B. , and R. L. Baldwin . 1964 . Helix-random coil transitions in DNA homopolymer pairs . J. Mol. Biol. 8 : 452 - 469 . (10.1016/S0022-2836(64)80003-6) / J. Mol. Biol. / Helix-random coil transitions in DNA homopolymer pairs by Inman R. B. (1964)
  46. Iwamura , T. , and J. Myers . 1959 . Changes in the content and distribution of the nucleic acid bases in Chlorella during the life cycle . Arch. Biochem. Biophys. 84 : 267 - 277 . (10.1016/0003-9861(59)90591-0) / Arch. Biochem. Biophys. / Changes in the content and distribution of the nucleic acid bases in Chlorella during the life cycle by Iwamura T. (1959)
  47. Jackson , J. F. , D. J. W. Moriarty , and D. J. D. Nicholas . 1968 . Deoxyribonucleic acid base composition and taxonomy of thiobacilli and some nitrifying bacteria . J. Gen. Microbiol. 53 : 53 - 60 . (10.1099/00221287-53-1-53) / J. Gen. Microbiol. / Deoxyribonucleic acid base composition and taxonomy of thiobacilli and some nitrifying bacteria by Jackson J. F. (1968)
  48. Kaiser , A. D. , and D. S. Hogness . 1960 . The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage ?dg . J. Mol. Biol. 2 : 392 - 415 . (10.1016/S0022-2836(60)80050-2) / J. Mol. Biol. / The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage ?dg by Kaiser A. D. (1960)
  49. Kaye , A. M. , R. Salomon , and B. Fridlender . 1967 . Base composition and presence of methylated bases in DNA from a blue-green alga Pkectonema boryanum . J. Mol. Biol. 24 : 479 - 483 . (10.1016/0022-2836(67)90234-3) / J. Mol. Biol. / Base composition and presence of methylated bases in DNA from a blue-green alga Pkectonema boryanum by Kaye A. M. (1967)
  50. Kingsbury , D. T. , and E. Weiss . 1968 . Lack of deoxyribonucleic acid homology between species of the genus Chlamydia . J. Bacteriol. 96 : 1421 - 1423 . (10.1128/jb.96.4.1421-1423.1968) / J. Bacteriol. / Lack of deoxyribonucleic acid homology between species of the genus Chlamydia by Kingsbury D. T. (1968)
  51. 1. Kirby , K. S. 1959 . The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method . Biochim. Biophys. Acta 36 : 117 - 124 . (10.1016/0006-3002(59)90075-7) / Biochim. Biophys. Acta / The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method by Kirby K. S. (1959)
  52. Kit , S. 1962 . Species differences in animal deoxyribonucleic acids as revealed by equilibrium sedimentation in density gradients . Nature 193 : 274 - 275 . (10.1038/193274a0) / Nature / Species differences in animal deoxyribonucleic acids as revealed by equilibrium sedimentation in density gradients by Kit S. (1962)
  53. Knight , C. A. 1954 . The chemical constitution of viruses . Advan. Virus Res. 2 : 153 - 182 . (10.1016/S0065-3527(08)60532-1) / Advan. Virus Res. / The chemical constitution of viruses by Knight C. A. (1954)
  54. Kogane , F. , and T. Yanagita . 1964 . Isolation and purification of deoxyribonucleic acid from Aspergillus oryzae conidia . J. Gen. Appl. Microbiol. 10 : 61 - 68 . (10.2323/jgam.10.61) / J. Gen. Appl. Microbiol. / Isolation and purification of deoxyribonucleic acid from Aspergillus oryzae conidia by Kogane F. (1964)
  55. LaMacchia E. H. , and M. J. Pelczar . 1966 . Analyses of deoxyribonucleic acid of Neisseria caviae and other Neisseria . J. Bacteriol. 91 : 514 - 516 . (10.1128/jb.91.2.514-516.1966) / J. Bacteriol. / Analyses of deoxyribonucleic acid of Neisseria caviae and other Neisseria by LaMacchia E. H. (1966)
  56. Lawrence , C. H. , and M. C. Clark . 1966 . Characterization of DNA from Streptomyces scabies . Can. J. Biochem. 44 : 1685 - 1688 . (10.1139/o66-190) / Can. J. Biochem. / Characterization of DNA from Streptomyces scabies by Lawrence C. H. (1966)
  57. Lawton , W. D. , B. C. Morris , and T. W. Burrows . 1968 . Gene transfer in strains of Pasteurella pseudotuberculosis . J. Gen. Microbiol. 52 : 25 - 34 . (10.1099/00221287-52-1-25) / J. Gen. Microbiol. / Gene transfer in strains of Pasteurella pseudotuberculosis by Lawton W. D. (1968)
  58. Lee , L. F. , and J. A. Boezi . 1966 . Characterization of bacteriophage gh-l for Pseudomonas putida . J. Bacteriol. 92 : 1821 - 1827 . (10.1128/jb.92.6.1821-1827.1966) / J. Bacteriol. / Characterization of bacteriophage gh-l for Pseudomonas putida by Lee L. F. (1966)
  59. Lee , K. Y. , R. Wahl , and E. Barbu . 1956 . Contenu en bases puriques et pyrimidiques des DNA des bacteries . Ann. Inst. Pasteur 91 : 212 - 224 . / Ann. Inst. Pasteur / Contenu en bases puriques et pyrimidiques des DNA des bacteries by Lee K. Y. (1956)
  60. Mandel , M. 1966 . Deoxyribonucleic acid base composition in the genus Pseudomonas . J. Gen. Microbiol. 43 : 273 - 292 . (10.1099/00221287-43-2-273) / J. Gen. Microbiol. / Deoxyribonucleic acid base composition in the genus Pseudomonas by Mandel M. (1966)
  61. Mandel , M. , and B. M. Honigberg . 1964 . Isolation and characterization of deoxyribonucleic acid of two species of Trkhomonas Donne . J. Protozool. 11 : 114 - 116 . (10.1111/j.1550-7408.1964.tb01730.x) / J. Protozool. / Isolation and characterization of deoxyribonucleic acid of two species of Trkhomonas Donne by Mandel M. (1964)
  62. Mandel , M. , and E. R. Leadbetter . 1965 . DNA base composition of myxobacteria . J. Bacteriol. 90 : 1795 - 1796 . (10.1128/jb.90.6.1795-1796.1965) / J. Bacteriol. / DNA base composition of myxobacteria by Mandel M. (1965)
  63. Mandel M. and R. Rownd. 1964. Deoxyribonucleic acid base composition in the Enterobacteriaceae: an evolutionary sequence? p. 585-597. In C. A. Leone (ed.) Taxonomic biochemistry and serology. Ronald Press Co. New York.
  64. Marmur , J. 1960 . Thermal denaturation of deoxyribonucleic acid isolated from a thermophile . Biochim. Biophys. Acta 38 : 342 - 343 . (10.1016/0006-3002(60)91251-8) / Biochim. Biophys. Acta / Thermal denaturation of deoxyribonucleic acid isolated from a thermophile by Marmur J. (1960)
  65. Marmur , J. , and P. Doty . 1962 . Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature . J. Mol. Biol. 5 : 109 - 118 . (10.1016/S0022-2836(62)80066-7) / J. Mol. Biol. / Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature by Marmur J. (1962)
  66. McDonald , W. C. , I. C. Felkner , A. Turetsky , and T. S. Matney . 1963 . Similarity in base compositions of deoxyribonucleates from several strains of Bacillus cereus and Bacillus anthracis . J. Bacteriol. 85 : 1071 - 1073 . (10.1128/jb.85.5.1071-1073.1963) / J. Bacteriol. / Similarity in base compositions of deoxyribonucleates from several strains of Bacillus cereus and Bacillus anthracis by McDonald W. C. (1963)
  67. Morowitz , H. J. , M. E. Tourtelotte , W. R. Guild , E. Castro , C. Woese , and R. C. Cleverdon . 1962 . The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, avian PPLO 5969 . J. Mol. Biol. 4 : 93 - 103 . (10.1016/S0022-2836(62)80041-2) / J. Mol. Biol. / The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, avian PPLO 5969 by Morowitz H. J. (1962)
  68. Neimark , H. C. , and J. J. Pene . 1965 . Characterization of Pleuropneumonia-like organisms by deoxyribonucleic acid composition . Proc. Soc. Exp. Biol. Med. 118 : 517 - 519 . (10.3181/00379727-118-29893) / Proc. Soc. Exp. Biol. Med. / Characterization of Pleuropneumonia-like organisms by deoxyribonucleic acid composition by Neimark H. C. (1965)
  69. Pifia , M. , and M. Green . 1965 . Biochemical studies on adenovirus multiplication . IX. Proc. Nat. Acad. Sci. U.S.A. 54 : 547 - 551 . / IX. Proc. Nat. Acad. Sci. U.S.A. / Biochemical studies on adenovirus multiplication by Pifia M. (1965)
  70. Prauser , H. 1966 . New and rare actinomycetes and their DNA base composition . Spisy Prirodoved Fak. Univ. J. E. Purkyne Brn. 475 : 268 - 270 . / Spisy Prirodoved Fak. Univ. J. E. Purkyne Brn. / New and rare actinomycetes and their DNA base composition by Prauser H. (1966)
  71. Prauser , H. , and R. Falta . 1968 . Phagensensibilitat, Zellwand-Zusammensetzung und Taxonomie von Actinomyceten . Z. Allg. Mikrobiol. 8 : 39 - 46 . (10.1002/jobm.3630080106) / Z. Allg. Mikrobiol. / Phagensensibilitat, Zellwand-Zusammensetzung und Taxonomie von Actinomyceten by Prauser H. (1968)
  72. Riley , M. , B. Maling , and M. Chamberlin . 1966 . Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes . J. Mol. Biol. 20 : 359 - 389 . (10.1016/0022-2836(66)90069-6) / J. Mol. Biol. / Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes by Riley M. (1966)
  73. Rosenkranz , H. S. , and P. D. Ellner . 1968 . Mutant of Bacterium paracoli 5099 with an altered DNA; identified as a Flavobacterium . Science 160 : 893 - 894 . (10.1126/science.160.3830.893) / Science / Mutant of Bacterium paracoli 5099 with an altered DNA; identified as a Flavobacterium by Rosenkranz H. S. (1968)
  74. Rost , K. , and H. Venner . 1964 . Untersuchungen an Nukleinsduren . X. Hoppe-Seyler's Z. Physiol. Chem. 339 : 230 - 237 . / X. Hoppe-Seyler's Z. Physiol. Chem. / Untersuchungen an Nukleinsduren by Rost K. (1964)
  75. Rosypalova , A. , J. Bohacek , and S. Rosypal . 1966 . Deoxyribonucleic acid base composition and taxonomy of violetpigmented cocci . Antonie Van Leeuwenhoek J. Microbiol. 32 : 105 - 112 . (10.1007/BF02097450) / Antonie Van Leeuwenhoek J. Microbiol. / Deoxyribonucleic acid base composition and taxonomy of violetpigmented cocci by Rosypalova A. (1966)
  76. Ruppel , H. G. , and D. von Wyck . 1965 . Ueber die Desoxyribonucleinesaure in Chloroplasten von Antirrhinum majus . Z. Pflanzenphysiol. 53 : 32 - 38 . / Z. Pflanzenphysiol. / Ueber die Desoxyribonucleinesaure in Chloroplasten von Antirrhinum majus by Ruppel H. G. (1965)
  77. Russell , W. C. and, L. V. Crawford . 1963 . Some characteristics of the deoxyribonucleic acid from herpes simplex virus . Virology 21 : 353 - 361 . (10.1016/0042-6822(63)90196-X) / Virology / Some characteristics of the deoxyribonucleic acid from herpes simplex virus by Russell W. C. (1963)
  78. Saunders , G. F. , and L. L. Campbell . 1966 . Deoxyribonucleic acid base composition of Desulfotomaculum nigrificans . J. Bacteriol. 92 : 515 . (10.1128/jb.92.2.515-515.1966) / J. Bacteriol. / Deoxyribonucleic acid base composition of Desulfotomaculum nigrificans by Saunders G. F. (1966)
  79. Saunders , G. F. , L. L. Campbell , and J. R. Postgate . 1964 . Base composition of deoxyribonucleic acid of sulfatereducing bacteria deduced from buoyant density measurements in cesium chloride . J. Bacteriol. 87 : 1073 - 1078 . (10.1128/jb.87.5.1073-1078.1964) / J. Bacteriol. / Base composition of deoxyribonucleic acid of sulfatereducing bacteria deduced from buoyant density measurements in cesium chloride by Saunders G. F. (1964)
  80. Schein , A. H. 1966 . The deoxyribonucleic acid of Micrococcus radiodurans . Biochem. J. 101 : 647 - 650 . (10.1042/bj1010647) / Biochem. J. / The deoxyribonucleic acid of Micrococcus radiodurans by Schein A. H. (1966)
  81. Schildkraut , C. L. , M. Mandel , S. Levisohn , J. E. Smith-Sonneborn , and J. Marmur . 1962 . Deoxyribonucleic acid base composition and taxonomy of some protozoa . Nature 196 : 795 - 796 . (10.1038/196795a0) / Nature / Deoxyribonucleic acid base composition and taxonomy of some protozoa by Schildkraut C. L. (1962)
  82. Schildkraut , C. L. , J. Marmur , and P. Doty . 1961 . The formation of hybrid DNA molecules and their use in studies of DNA homologies . J. Mol. Biol. 3 : 595 - 617 . (10.1016/S0022-2836(61)80024-7) / J. Mol. Biol. / The formation of hybrid DNA molecules and their use in studies of DNA homologies by Schildkraut C. L. (1961)
  83. Schildkraut , C. L. , J. Marmur , and P. Doty . 1962 . Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCI . J. Mol. Biol. 4 : 430 - 443 . (10.1016/S0022-2836(62)80100-4) / J. Mol. Biol. / Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCI by Schildkraut C. L. (1962)
  84. Sebald , M. , and M. Wron . 1963 . Teneur en bases de 1'ADN et classification des vibrions . Ann. Inst. Pasteur 105 : 897 - 910 . / Ann. Inst. Pasteur / Teneur en bases de 1'ADN et classification des vibrions by Sebald M. (1963)
  85. Sigal , N. , J. Senez , J. Le Gall , and M. Sebald . 1963 . Base composition of the deoxyribonucleic acid of sulfate reducing bacteria . J. Bacteriol. 85 : 1315 - 1318 . (10.1128/jb.85.6.1315-1318.1963) / J. Bacteriol. / Base composition of the deoxyribonucleic acid of sulfate reducing bacteria by Sigal N. (1963)
  86. Smith , M. 1963 . Deoxyribonucleic acids in crabs of the genus Cancer. Biochim. Biophys. Res. Commun. 10 : 67 - 72 . (10.1016/0006-291X(63)90270-5) / Cancer. Biochim. Biophys. Res. Commun. / Deoxyribonucleic acids in crabs of the genus by Smith M. (1963)
  87. Smith , M. 1964 . Deoxyribonucleic acids of crustacea . J. Mol. Biol. 9 : 17 - 23 . (10.1016/S0022-2836(64)80088-7) / J. Mol. Biol. / Deoxyribonucleic acids of crustacea by Smith M. (1964)
  88. Smith , M. , and D. B. Quayle . 1963 . Deoxyribonucleic acids of marine mollusca . Nature 200 : 676 . (10.1038/200676a0) / Nature / Deoxyribonucleic acids of marine mollusca by Smith M. (1963)
  89. Smith , J. D. , and G. R. Wyatt . 1951 . The composition of some microbial deoxypentose nucleic acids . Biochem. J. 49 : 144 - 148 . (10.1042/bj0490144) / Biochem. J. / The composition of some microbial deoxypentose nucleic acids by Smith J. D. (1951)
  90. Stenesh , J. , B. A. Roe , and T. L. Snyder . 1968 . Studies of the deoxyribonucleic acid from mesophilic and thermophilic bacteria . Biochim. Biophys. Acta 161 : 442 - 454 . (10.1016/0005-2787(68)90121-4) / Biochim. Biophys. Acta / Studies of the deoxyribonucleic acid from mesophilic and thermophilic bacteria by Stenesh J. (1968)
  91. Sueoka , N. 1961 . Variation and heterogeneity of base composition of deoxyribonucleic acids; a compilation ot old and new data . J. Mol. Biol. 3 : 31 - 40 . (10.1016/S0022-2836(61)80005-3) / J. Mol. Biol. / Variation and heterogeneity of base composition of deoxyribonucleic acids; a compilation ot old and new data by Sueoka N. (1961)
  92. Takayama , K. , S. Abe , and S. Kinoshita . 1965 . Taxonomic studies on glutamic acid producing bacteria. Ill. On the base composition of DNA . J. Agr. Chem. Soc. Jap. 39 : 342 - 346 . / J. Agr. Chem. Soc. Jap. / Taxonomic studies on glutamic acid producing bacteria. Ill. On the base composition of DNA by Takayama K. (1965)
  93. Tewfik , E. M. , and S. G. Bradley . 1967 . Characterization of deoxyribonucleic acids from streptomycetes and nocardiac . J. Bacteriol. 94 : 1994 - 2000 . (10.1128/jb.94.6.1994-2000.1967) / J. Bacteriol. / Characterization of deoxyribonucleic acids from streptomycetes and nocardiac by Tewfik E. M. (1967)
  94. Tonomura , B. , R. Malkin , and J. C. Rabinowitz . 1965 . Deoxyribonucleic acid base composition and clostridial species . J. Bacteriol. 89 : 1438 - 1439 . (10.1128/jb.89.5.1438-1439.1965) / J. Bacteriol. / Deoxyribonucleic acid base composition and clostridial species by Tonomura B. (1965)
  95. Vanjushin , B. F. , Belozersky, A. N. , and N. A. Kokurina . 1966 . Nucleic acids and plant evolution . Trans. Moscow Soc. Nature 24 : 7 - 25 . / Trans. Moscow Soc. Nature / Nucleic acids and plant evolution by Vanjushin B. F. (1966)
  96. Volkin , E. , L. Astrachan , and J. L. Countryman . 1958 . Metabolism of RNA phosphorus in Escherichia coli infected with bacteriophage T 7 . Virology 6 : 545 - 555 . (10.1016/0042-6822(58)90101-6) / Virology / Metabolism of RNA phosphorus in Escherichia coli infected with bacteriophage T 7 by Volkin E. (1958)
  97. Wagenbreth , D. 1961 . Ein Beitrag zur systematischeni Einordnung der Knbllchenbakterien durch Bestimmung des relativen Basengehaltes ihrer Desoxyribonucleinsauren . Flora 151 : 219 - 230 . / Flora / Ein Beitrag zur systematischeni Einordnung der Knbllchenbakterien durch Bestimmung des relativen Basengehaltes ihrer Desoxyribonucleinsauren by Wagenbreth D. (1961)
  98. Watson , J. D. , and J. W. Littlefield . 1960 . Some properties of DNA from Shope papilloma virus . J. Mol. Biol. 2 : 161 - 165 . (10.1016/S0022-2836(60)80018-6) / J. Mol. Biol. / Some properties of DNA from Shope papilloma virus by Watson J. D. (1960)
  99. Wayne , L. G. , and W. M. Gross . 1968 . Isolation of deoxyribonucleic acid from mycobacteria . J. Bacteriol. 95 : 1481 - 1482 . (10.1128/jb.95.4.1481-1482.1968) / J. Bacteriol. / Isolation of deoxyribonucleic acid from mycobacteria by Wayne L. G. (1968)
  100. Weed , L. L. 1963 . Effects of copper on Bacillus subtilis . J. Bacteriol. 85 : 1003 - 1010 . (10.1128/jb.85.5.1003-1010.1963) / J. Bacteriol. / Effects of copper on Bacillus subtilis by Weed L. L. (1963)
  101. Weil , R. 1963 . The denaturation and the renaturation of the DNA of polyoma virus . Proc. Nat. Acad. Sci. U.S.A. 49 : 480 - 486 . (10.1073/pnas.49.4.480) / Proc. Nat. Acad. Sci. U.S.A. / The denaturation and the renaturation of the DNA of polyoma virus by Weil R. (1963)
  102. Welker , N. E. , and L. L. Campbell . 1967 . Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis . J. Bacteriol. 94 : 1124 - 1130 . (10.1128/jb.94.4.1124-1130.1967) / J. Bacteriol. / Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis by Welker N. E. (1967)
  103. Wells , R. D. , E. Ohtsuka , and H. G. Khorana . 1965 . Studies on polynucleotides . J. Mol. Biol. 14 : 221 - 240 . (10.1016/S0022-2836(65)80242-X) / J. Mol. Biol. / Studies on polynucleotides by Wells R. D. (1965)
  104. Wyatt , G. R. 1951 . The purine and pyrimidine composition of deoxypentose nucleic acids . Biochem. J. 48 : 584 - 590 . (10.1042/bj0480584) / Biochem. J. / The purine and pyrimidine composition of deoxypentose nucleic acids by Wyatt G. R. (1951)
  105. Wyatt , G. R. , and S. S. Cohen . 1952 . Nucleic acids of Rickettsiae . Nature 170 : 846 - 847 . (10.1038/170846a0) / Nature / Nucleic acids of Rickettsiae by Wyatt G. R. (1952)
  106. Wyatt , G. R. , and S. S. Cohen . 1953 . The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine . Biochem. J. 55 : 774 - 782 . (10.1042/bj0550774) / Biochem. J. / The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine by Wyatt G. R. (1953)
  107. Yamaguchi , T. 1967 . Similarity in DNA of various morphologically distinct actinomycetes . J. Geni. Appl. Microbiol. 13 : 63 - 71 . (10.2323/jgam.13.63) / J. Geni. Appl. Microbiol. / Similarity in DNA of various morphologically distinct actinomycetes by Yamaguchi T. (1967)
Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 8:35 a.m.)
Deposited 4 years ago (July 29, 2021, 12:56 p.m.)
Indexed 1 year ago (Aug. 3, 2024, 6:50 p.m.)
Issued 55 years, 5 months ago (March 1, 1970)
Published 55 years, 5 months ago (March 1, 1970)
Published Print 55 years, 5 months ago (March 1, 1970)
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@article{De_Ley_1970, title={Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid}, volume={101}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.101.3.738-754.1970}, DOI={10.1128/jb.101.3.738-754.1970}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={De Ley, J.}, year={1970}, month=mar, pages={738–754} }