DOI: 10.1128/jb.100.3.1371-1377.1969. Pyrimidine Synthesis in <i>Neurospora crassa:</i> Gene-Enzyme Relationships

Pyrimidine Synthesis in Neurospora crassa: Gene-Enzyme Relationships

10.1128/jb.100.3.1371-1377.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A new series of pyrimidine-requiring mutants of Neurospora has been isolated and all enzymes involved in pyrimidine biosynthesis are represented by at least one mutant. Among these mutants is included a single isolate for a new locus, pyr-6 . This mutant is deficient in dihydroorotase (DHOase) and represents the only enzymatic step in orotate synthesis for which no mutant previously had been found. This mutant, which mapped genetically on the right arm of linkage group V, is unlinked to any of the other pyrimidine mutants. The DHOase-deficient mutant is also characterized by an unexpected growth behavior. The pyr-1 locus has been specifically associated with a lack of dihydroorotate dehydrogenase (DHOdehase). Mutants isolated in this series for other pyrimidine loci have been related to previously isolated mutants by allelism, recombination, and accumulation studies.

Bibliography

Caroline, D. F. (1969). Pyrimidine Synthesis in Neurospora crassa: Gene-Enzyme Relationships. Journal of Bacteriology, 100(3), 1371â1377.

Authors 1

Dina F. Caroline (first)

References 27 Referenced 33

Caroline , D. F. , and R. H. Davis . 1969 . Pyrimidine synthesis in Neurospora crassa: regulation of enzyme activities . J. Bacteriol. 100 : 1378 - 1384 . (10.1128/jb.100.3.1378-1384.1969) / J. Bacteriol. / Pyrimidine synthesis in Neurospora crassa: regulation of enzyme activities by Caroline D. F. (1969)
Davis , R. H. 1960 . An enzymatic difference among pyr-3 mutants of Neurospora . Proc. Nat. Acad. Sci. U.S.A. 46 : 677 - 682 . (10.1073/pnas.46.5.677) / Proc. Nat. Acad. Sci. U.S.A. / An enzymatic difference among pyr-3 mutants of Neurospora by Davis R. H. (1960)
Davis , R. H. 1965 . Carbamyl phosphate synthesis in Neurospora crassa. II. Genetics, metabolic position, and regulation of arginine-specific carbamyl phosphokinase . Biochim. Biophys. Acta 107 : 54 - 68 . (10.1016/0304-4165(65)90388-0) / Biochim. Biophys. Acta / Carbamyl phosphate synthesis in Neurospora crassa. II. Genetics, metabolic position, and regulation of arginine-specific carbamyl phosphokinase by Davis R. H. (1965)
Davis , R. H. 1967 . Channeling in Neurospora metabolism, p. 303-322 . In H. J. Vogel , L. 0 . Lampen and V. Bryson (ed.), Organizational Biosynthesis. Academic Press Inc., New York. / H. J. Vogel / Channeling in Neurospora metabolism, p. 303-322 by Davis R. H. (1967)
Davis , R. H. , and V. W. Woodward . 1962 . The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora . Genetics 47 : 1075 - 1083 . (10.1093/genetics/47.8.1075) / Genetics / The relationship between gene suppression and aspartate transcarbamylase activity in pyr-3 mutants of Neurospora by Davis R. H. (1962)
Finck , D. , Y. Suyama , and R. H. Davis . 1965 . Metabolic role of the pyrimidine-3 locus of Neurospora . Genetics 52 : 829 - 839 . (10.1093/genetics/52.4.829) / Genetics / Metabolic role of the pyrimidine-3 locus of Neurospora by Finck D. (1965)
Gerhart , J. C. , and A. B. Pardee . 1962 . The enzymology of control by feedback inhibition . J. Biol. Chem. 237 : 891 - 896 . (10.1016/S0021-9258(18)60389-8) / J. Biol. Chem. / The enzymology of control by feedback inhibition by Gerhart J. C. (1962)
Hayward , W. S. , and W. L. Belser . 1965 . Regulation of pyrimidine biosynthesis in Serratia marcescens . Proc. Nat. Acad. Sci. U.S.A. 52 : 1483 - 1489 . (10.1073/pnas.53.6.1483) / Proc. Nat. Acad. Sci. U.S.A. / Regulation of pyrimidine biosynthesis in Serratia marcescens by Hayward W. S. (1965)
Howell , R. R. , J. R. Klinenberg , and R. S. Krooth . 1967 . Enzyme studies on diploid cell strains developed from patients with hereditary orotic aciduria . The Johns Hopkins Med. J. 120 : 81 - 88 . / The Johns Hopkins Med. J. / Enzyme studies on diploid cell strains developed from patients with hereditary orotic aciduria by Howell R. R. (1967)
Jones , M. E. 1963 . Carbamyl phosphate . Science 140 : 1373 - 1379 . (10.1126/science.140.3574.1373) / Science / Carbamyl phosphate by Jones M. E. (1963)
Koritz , S. B. , and P. P. Cohen . 1954 . Colorimetric determination of carbamylamino acids and related compounds . J. Biol. Chem. 209 : 145 - 150 . (10.1016/S0021-9258(18)65539-5) / J. Biol. Chem. / Colorimetric determination of carbamylamino acids and related compounds by Koritz S. B. (1954)
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Layne E. 1955. Spectrophotometric and turbidimetric methods for measuring proteins p. 447. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 3. Academic Press Inc. New York.
Michelson , A. M. , W. Drell , and H. K. Mitchell . 1951 . A new ribose nucleoside from Neurospora: "orotidine ." Proc. Nat. Acad. Sci. U.S.A. , 37 : 396 - 399 . (10.1073/pnas.37.7.396) / Proc. Nat. Acad. Sci. U.S.A. / A new ribose nucleoside from Neurospora: "orotidine by Michelson A. M. (1951)
Miller , R. W. , and C. T. Kerr . 1967 . Particulate dihydroorotate oxidase system from a pseudomonad . Can. J. Biochem. 45 : 1283 - 1294 . (10.1139/o67-150) / Can. J. Biochem. / Particulate dihydroorotate oxidase system from a pseudomonad by Miller R. W. (1967)
Mitchell , H. K. , M. B. Houlahan , and J. F. Nyc . 1948 . The accumulation of orotic acid by a pyrimidineless mutant of Neurospora . J. Biol. Chem. 172 : 525 - 529 . (10.1016/S0021-9258(19)52738-7) / J. Biol. Chem. / The accumulation of orotic acid by a pyrimidineless mutant of Neurospora by Mitchell H. K. (1948)
Perkins , D. D. 1964 . Multiple interchange stocks for linkage detection . Neurospora Newsl. 6 : 22 . / Neurospora Newsl. / Multiple interchange stocks for linkage detection by Perkins D. D. (1964)
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Reissig , J. L. 1963 . Spectrum of forward mutants in the pyr-3 region of Neurospora . J. Gen. Microbiol. 30 : 327 - 337 . (10.1099/00221287-30-2-327) / J. Gen. Microbiol. / Spectrum of forward mutants in the pyr-3 region of Neurospora by Reissig J. L. (1963)
Smith , L. H. , Jr. , and F. A. Baker . 1959 . Pyrimidine metabolism in man. I. The biosynthesis of orotic acid . J. Clin. Invest. 38 : 798 - 809 . (10.1172/JCI103862) / J. Clin. Invest. / Pyrimidine metabolism in man. I. The biosynthesis of orotic acid by Smith L. H. (1959)
Taylor , W. H. , and M. L. Taylor . 1964 . Enzymes of the pyrimidine pathway in Escherichia coli. II. Intracellular localization and properties of dihydroorotic dehydrogenase . J. Bacteriol. 88 : 105 - 101 10. (10.1128/jb.88.1.105-110.1964) / J. Bacteriol. / Enzymes of the pyrimidine pathway in Escherichia coli. II. Intracellular localization and properties of dihydroorotic dehydrogenase by Taylor W. H. (1964)
Taylor , W. H. , M. L. Taylor , and D. F. Eames . 1966 . Two functionally different dihydroorotic dehydrogenases in bacteria . J. Bacteriol. 91 : 2251 - 2256 . (10.1128/jb.91.6.2251-2256.1966) / J. Bacteriol. / Two functionally different dihydroorotic dehydrogenases in bacteria by Taylor W. H. (1966)
Vogel , H. J. 1956 . A convenient growth medium for Neurospora (Medium N) . Microbiol. Genet. Bull. 13 : 42 - 43 . / Microbiol. Genet. Bull. / A convenient growth medium for Neurospora (Medium N) by Vogel H. J. (1956)
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Woodward , V. W. , J. R. de Zeeuw , and A. M. Srb . 1954 . The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating . Proc. Nat. Acad. Sci. U.S.A. 40 : 192 - 200 . (10.1073/pnas.40.3.192) / Proc. Nat. Acad. Sci. U.S.A. / The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating by Woodward V. W. (1954)
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Yates , R. A. , and A. B. Pardee . 1956 . Pyrimidine biosynthesis in Escherichia coli . J. Biol. Chem. 221 : 743 - 756 . (10.1016/S0021-9258(18)65187-7) / J. Biol. Chem. / Pyrimidine biosynthesis in Escherichia coli by Yates R. A. (1956)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:23 a.m.)
Deposited	4 years, 2 months ago (June 11, 2021, 10:28 p.m.)
Indexed	2 years, 4 months ago (April 2, 2023, 7:29 p.m.)
Issued	55 years, 8 months ago (Dec. 1, 1969)
Published	55 years, 8 months ago (Dec. 1, 1969)
Published Print	55 years, 8 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Caroline_1969, title={Pyrimidine Synthesis in Neurospora crassa: Gene-Enzyme Relationships}, volume={100}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.100.3.1371-1377.1969}, DOI={10.1128/jb.100.3.1371-1377.1969}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Caroline, Dina F.}, year={1969}, month=dec, pages={1371–1377} }

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