DOI: 10.1128/jb.100.3.1316-1321.1969. Initial Characterization of a Temperature-Sensitive Rod <sup>−</sup> Mutant of <i>Bacillus subtilis</i>

Initial Characterization of a Temperature-Sensitive Rod − Mutant of Bacillus subtilis

10.1128/jb.100.3.1316-1321.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The general biological properties of a temperature-sensitive morphological mutant of Bacillus subtilis (168ts–200B) are described. At the restrictive temperature (45 C), cells grow as spheres which divide irregularly to form grapelike clusters. At the permissive temperature (30 C), the mutant grows as typical B. subtilis rods in short chains. A log-phase culture of rods (30 C) may be converted to spheres by transfer to 45 C. Reversion of spheres to rods occurs when the alternate temperature shift is made. Growth curves, deoxyribonucleic acid replication kinetics, and the morphology of mutant 168ts–200B are described.

Bibliography

Boylan, R. J., & Mendelson, N. H. (1969). Initial Characterization of a Temperature-Sensitive Rod â Mutant of Bacillus subtilis. Journal of Bacteriology, 100(3), 1316â1321.

Authors 2

Robert J. Boylan (first)
Neil H. Mendelson (additional)

References 11 Referenced 56

Adler , H. I. , W. D. Fisher , A. Cohen , and A. A. Hardigree . 1967 . Miniature Escherichia coli cells deficient in DNA . Proc. Nat. Acad. Sci. U.S.A. 57 : 321 - 326 . (10.1073/pnas.57.2.321) / Proc. Nat. Acad. Sci. U.S.A. / Miniature Escherichia coli cells deficient in DNA by Adler H. I. (1967)
Adler , H. I. , and A. A. Hardigree . 1964 . Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli . J. Bacteriol. 87 : 720 - 726 . (10.1128/jb.87.3.720-726.1964) / J. Bacteriol. / Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli by Adler H. I. (1964)
Adler , H. I. , C. E. Terry , and A. A. Hardigree . 1968 . Giant cells of Escherichia coli . J. Bacteriol. 95 : 134 - 142 . (10.1128/jb.95.1.139-142.1968) / J. Bacteriol. / Giant cells of Escherichia coli by Adler H. I. (1968)
Gross , J. D. , D. Karamata , and P. G. Hempstead . 1968 . Temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis . Cold Spring Harbor Symp. Quant. Biol. 33 : 307 - 312 . (10.1101/SQB.1968.033.01.034) / Cold Spring Harbor Symp. Quant. Biol. / Temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis by Gross J. D. (1968)
Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Thermosensitive mutants of Escherichia coli affected in the process of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 693 . (10.1101/SQB.1968.033.01.077) / Cold Spring Harbor Symp. Quant. Biol. / Thermosensitive mutants of Escherichia coli affected in the process of DNA synthesis and cellular division by Hirota Y. (1968)
Howard-Flanders , P. , E. Simson , and L. Theriot . 1964 . A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12 . Genetics 49 : 237 - 246 . (10.1093/genetics/49.2.237) / Genetics / A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12 by Howard-Flanders P. (1964)
Mendelson , N. H. , and J. D. Gross . 1967 . Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication . J. Bacteriol. 94 : 1603 - 1608 . (10.1128/jb.94.5.1603-1608.1967) / J. Bacteriol. / Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication by Mendelson N. H. (1967)
Rogers , H. J. , M. McConnell , and I. D. J. Burdett . 1968 . Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology . Nature 219 : 285 - 288 . (10.1038/219285a0) / Nature / Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology by Rogers H. J. (1968)
Walker , J. R. , and A. B. Pardee . 1967 . Conditional mutants involving septum formation in Escherichia coll . J. Bacteriol. 93 : 107 - 114 . (10.1128/jb.93.1.107-114.1967) / J. Bacteriol. / Conditional mutants involving septum formation in Escherichia coll by Walker J. R. (1967)
Walker , J. R. , and A. B. Pardee . 1968 . Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli . J. Bacteriol. 95 : 123 - 131 . (10.1128/jb.95.1.123-131.1968) / J. Bacteriol. / Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli by Walker J. R. (1968)
Yoshikawa , H. 1965 . DNA synthesis during germination of Bacillus subtilis spores . Proc. Nat. Acad. Sci. U.S.A. 53 : 1476 - 1483 . (10.1073/pnas.53.6.1476) / Proc. Nat. Acad. Sci. U.S.A. / DNA synthesis during germination of Bacillus subtilis spores by Yoshikawa H. (1965)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 8:35 a.m.)
Deposited	3 years, 11 months ago (Sept. 3, 2021, 10:57 p.m.)
Indexed	1 year, 10 months ago (Oct. 14, 2023, 3:41 a.m.)
Issued	55 years, 9 months ago (Dec. 1, 1969)
Published	55 years, 9 months ago (Dec. 1, 1969)
Published Print	55 years, 9 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Boylan_1969, title={Initial Characterization of a Temperature-Sensitive Rod − Mutant of Bacillus subtilis}, volume={100}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.100.3.1316-1321.1969}, DOI={10.1128/jb.100.3.1316-1321.1969}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Boylan, Robert J. and Mendelson, Neil H.}, year={1969}, month=dec, pages={1316–1321} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        14
      ]
    ],
    "date-time": "2023-10-14T07:41:57Z",
    "timestamp": 1697269317007
  },
  "reference-count": 11,
  "publisher": "American Society for Microbiology",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1969,
            12,
            1
          ]
        ],
        "date-time": "1969-12-01T00:00:00Z",
        "timestamp": -2678400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  },
  "abstract": "<jats:p>\n            The general biological properties of a temperature-sensitive morphological mutant of\n            <jats:italic>Bacillus subtilis</jats:italic>\n            (168ts\u2013200B) are described. At the restrictive temperature (45 C), cells grow as spheres which divide irregularly to form grapelike clusters. At the permissive temperature (30 C), the mutant grows as typical\n            <jats:italic>B. subtilis</jats:italic>\n            rods in short chains. A log-phase culture of rods (30 C) may be converted to spheres by transfer to 45 C. Reversion of spheres to rods occurs when the alternate temperature shift is made. Growth curves, deoxyribonucleic acid replication kinetics, and the morphology of mutant 168ts\u2013200B are described.\n          </jats:p>",
  "DOI": "10.1128/jb.100.3.1316-1321.1969",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T13:35:10Z",
    "timestamp": 1578058510000
  },
  "page": "1316-1321",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 56,
  "title": "Initial Characterization of a Temperature-Sensitive Rod\n            <sup>\u2212</sup>\n            Mutant of\n            <i>Bacillus subtilis</i>",
  "prefix": "10.1128",
  "volume": "100",
  "author": [
    {
      "given": "Robert J.",
      "family": "Boylan",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Microbiology, University of Maryland, School of Dentistry, Baltimore, Maryland 21201"
        }
      ]
    },
    {
      "given": "Neil H.",
      "family": "Mendelson",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Division of Biological Sciences, University of Maryland, Baltimore County, Maryland 21228"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1073/pnas.57.2.321",
      "article-title": "Miniature Escherichia coli cells deficient in DNA",
      "volume": "57",
      "author": "Adler H. I.",
      "year": "1967",
      "unstructured": "Adler , H. I. , W. D. Fisher , A. Cohen , and A. A. Hardigree . 1967 . Miniature Escherichia coli cells deficient in DNA . Proc. Nat. Acad. Sci. U.S.A. 57 : 321 - 326 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "720",
      "DOI": "10.1128/jb.87.3.720-726.1964",
      "article-title": "Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli",
      "volume": "87",
      "author": "Adler H. I.",
      "year": "1964",
      "unstructured": "Adler , H. I. , and A. A. Hardigree . 1964 . Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli . J. Bacteriol. 87 : 720 - 726 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "134",
      "DOI": "10.1128/jb.95.1.139-142.1968",
      "article-title": "Giant cells of Escherichia coli",
      "volume": "95",
      "author": "Adler H. I.",
      "year": "1968",
      "unstructured": "Adler , H. I. , C. E. Terry , and A. A. Hardigree . 1968 . Giant cells of Escherichia coli . J. Bacteriol. 95 : 134 - 142 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "307",
      "DOI": "10.1101/SQB.1968.033.01.034",
      "article-title": "Temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis",
      "volume": "33",
      "author": "Gross J. D.",
      "year": "1968",
      "unstructured": "Gross , J. D. , D. Karamata , and P. G. Hempstead . 1968 . Temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis . Cold Spring Harbor Symp. Quant. Biol. 33 : 307 - 312 .",
      "journal-title": "Cold Spring Harbor Symp. Quant. Biol."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "677",
      "DOI": "10.1101/SQB.1968.033.01.077",
      "article-title": "Thermosensitive mutants of Escherichia coli affected in the process of DNA synthesis and cellular division",
      "volume": "33",
      "author": "Hirota Y.",
      "year": "1968",
      "unstructured": "Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Thermosensitive mutants of Escherichia coli affected in the process of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 693 .",
      "journal-title": "Cold Spring Harbor Symp. Quant. Biol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1093/genetics/49.2.237",
      "article-title": "A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12",
      "volume": "49",
      "author": "Howard-Flanders P.",
      "year": "1964",
      "unstructured": "Howard-Flanders , P. , E. Simson , and L. Theriot . 1964 . A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12 . Genetics 49 : 237 - 246 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "1603",
      "DOI": "10.1128/jb.94.5.1603-1608.1967",
      "article-title": "Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication",
      "volume": "94",
      "author": "Mendelson N. H.",
      "year": "1967",
      "unstructured": "Mendelson , N. H. , and J. D. Gross . 1967 . Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication . J. Bacteriol. 94 : 1603 - 1608 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "285",
      "DOI": "10.1038/219285a0",
      "article-title": "Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology",
      "volume": "219",
      "author": "Rogers H. J.",
      "year": "1968",
      "unstructured": "Rogers , H. J. , M. McConnell , and I. D. J. Burdett . 1968 . Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology . Nature 219 : 285 - 288 .",
      "journal-title": "Nature"
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1128/jb.93.1.107-114.1967",
      "article-title": "Conditional mutants involving septum formation in Escherichia coll",
      "volume": "93",
      "author": "Walker J. R.",
      "year": "1967",
      "unstructured": "Walker , J. R. , and A. B. Pardee . 1967 . Conditional mutants involving septum formation in Escherichia coll . J. Bacteriol. 93 : 107 - 114 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1128/jb.95.1.123-131.1968",
      "article-title": "Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli",
      "volume": "95",
      "author": "Walker J. R.",
      "year": "1968",
      "unstructured": "Walker , J. R. , and A. B. Pardee . 1968 . Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli . J. Bacteriol. 95 : 123 - 131 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "1476",
      "DOI": "10.1073/pnas.53.6.1476",
      "article-title": "DNA synthesis during germination of Bacillus subtilis spores",
      "volume": "53",
      "author": "Yoshikawa H.",
      "year": "1965",
      "unstructured": "Yoshikawa , H. 1965 . DNA synthesis during germination of Bacillus subtilis spores . Proc. Nat. Acad. Sci. U.S.A. 53 : 1476 - 1483 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.100.3.1316-1321.1969",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.100.3.1316-1321.1969",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        9,
        4
      ]
    ],
    "date-time": "2021-09-04T02:57:26Z",
    "timestamp": 1630724246000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.100.3.1316-1321.1969"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  },
  "references-count": 11,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1969,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.100.3.1316-1321.1969"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.100.3.1316-1321.1969",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}