DOI: 10.1128/jb.100.1.231-239.1969. Characteristics of Some Multiply Recombination-Deficient Strains of <i>Escherichia coli</i>

Characteristics of Some Multiply Recombination-Deficient Strains of Escherichia coli

10.1128/jb.100.1.231-239.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA + , recC22 recB21 recA13, recC22 recB + recA13 , and recC + recB21 recA13 . All multiple rec − strains carrying recA13 were similar to AB2463, which carries recA13 alone, in their UV sensitivities, recombination deficiencies, and inabilities to induce lambda phage in a lysogen. However, whereas AB2463 shows a high rate of ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown, the multiple rec − strains showed the low level characteristic of strains carrying recC22 or recB21 alone. The strain carrying both recC22 and recB21 was similar in all properties to the single mutants, suggesting that both gene products act in the same part of the recombination and UV repair pathways. It is concluded that in a Rec + strain, the recA + product acts to inhibit DNA breakdown determined by the recC + and recB + products.

Bibliography

Willetts, N. S., & Clark, A. J. (1969). Characteristics of Some Multiply Recombination-Deficient Strains of Escherichia coli. Journal of Bacteriology, 100(1), 231â239.

Authors 2

N. S. Willetts (first)
A. J. Clark (additional)

References 15 Referenced 225

Adelberg , E. A. , and S. N. Burns . 1960 . Genetic variation in the sex factor of Escherichia coli . J. Bacteriol. 79 : 321 - 330 . (10.1128/jb.79.3.321-330.1960) / J. Bacteriol. / Genetic variation in the sex factor of Escherichia coli by Adelberg E. A. (1960)
Brooks , K. , and A. J. Clark . 1967 . Behavior of bacteriophage in a recombination deficient strain of Escherichia coli . J. Virol. 1 : 283 - 293 . (10.1128/jvi.1.2.283-293.1967) / J. Virol. / Behavior of bacteriophage in a recombination deficient strain of Escherichia coli by Brooks K. (1967)
Buttin , G. , and M. R. Wright . 1968 . DNA breakdown in E. coli . Cold Spring Harbor Symp. Quant. Biol. 33 : 259 - 269 . / Cold Spring Harbor Symp. Quant. Biol. / DNA breakdown in E. coli by Buttin G. (1968)
Clark A. J. 1967. The beginning of a genetic analysis of recombination proficiency. J. Cell. Physiol. 70(Suppl.)1: 165-180. (10.1002/jcp.1040700412)
Clark , A. J. , M. Chamberlin , R. P. Boyce , and P. Howard-Flanders . 1966 . Abnormal metabolic response to ultraviolet light of a recombination-deficient mutant of Escherichia coli K12 . J. Mol. Biol. 19 : 442 - 454 . (10.1016/S0022-2836(66)80015-3) / J. Mol. Biol. / Abnormal metabolic response to ultraviolet light of a recombination-deficient mutant of Escherichia coli K12 by Clark A. J. (1966)
Clark , A. J. , and A. D. Margulies . 1965 . Isolation and characterization of recombination-deficient mutants of Escherichia coli K12 . Proc. Nat. Acad. Sci. U.S.A. 53 : 451 - 459 . (10.1073/pnas.53.2.451) / Proc. Nat. Acad. Sci. U.S.A. / Isolation and characterization of recombination-deficient mutants of Escherichia coli K12 by Clark A. J. (1965)
Emmerson , P. T. 1968 . Recombination deficient mutants of Escherichia coli K12 that map between thyA and argA . Genetics 60 : 19 - 30 . (10.1093/genetics/60.1.19) / Genetics / Recombination deficient mutants of Escherichia coli K12 that map between thyA and argA by Emmerson P. T. (1968)
Emmerson , P. T. , and P. Howard-Flanders . 1967 . Cotransduction with thy of a gene required for genetic recombination in E. coli K-12 . J. Bacteriol. 93 : 1729 - 1731 . (10.1128/jb.93.5.1729-1731.1967) / J. Bacteriol. / Cotransduction with thy of a gene required for genetic recombination in E. coli K-12 by Emmerson P. T. (1967)
Hertman , I. , and S. E. Luria . 1967 . TransJuction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda . J. Mol. Biol. 23 : 117 - 133 . (10.1016/S0022-2836(67)80021-4) / J. Mol. Biol. / TransJuction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda by Hertman I. (1967)
Howard-Flanders , P. , and R. P. Boyce . 1966 . DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes . Radiat. Res. 6(Suppl. ): 156 - 184 . / Radiat. Res. / DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes by Howard-Flanders P. (1966)
Howard-Flanders , P. , and L. Theriot . 1966 . Mutants of Escherichia coli K12 defective in DNA repair and in genetic recombination . Genetics 53 : 1137 - 1150 . (10.1093/genetics/53.6.1137) / Genetics / Mutants of Escherichia coli K12 defective in DNA repair and in genetic recombination by Howard-Flanders P. (1966)
Ila . Howard-Flanders, P. , L. Theriot , and J. B. Stedeford . 1969 . Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12 . J. Bacteriol. 97 : 1134 - 1141 . (10.1128/jb.97.3.1134-1141.1969) / J. Bacteriol. / Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12 by Ila P. (1969)
Low , B. 1968 . Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12 . Proc. Natl. Acad. Sci. U.S.A. 60 : 160 - 167 . (10.1073/pnas.60.1.160) / Proc. Natl. Acad. Sci. U.S.A. / Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12 by Low B. (1968)
Taylor , A. L. , and C. D. Trotter . 1967 . Revised linkage map of Escherichia coli . Bacteriol. Rev. 31 : 332 - 353 . (10.1128/br.31.4.332-353.1967) / Bacteriol. Rev. / Revised linkage map of Escherichia coli by Taylor A. L. (1967)
Willetts , N. S. , A. J. Clark , and B. Low . 1969 . Genetic location of certain mutations conferring recombination deficiency in Escherichia coli . J. Bacteriol. 97 : 244 - 249 . (10.1128/jb.97.1.244-249.1969) / J. Bacteriol. / Genetic location of certain mutations conferring recombination deficiency in Escherichia coli by Willetts N. S. (1969)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:22 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:49 p.m.)
Indexed	6 days, 15 hours ago (Aug. 23, 2025, 9:54 p.m.)
Issued	55 years, 10 months ago (Oct. 1, 1969)
Published	55 years, 10 months ago (Oct. 1, 1969)
Published Print	55 years, 10 months ago (Oct. 1, 1969)

Funders 0

None

BibTeX

@article{Willetts_1969, title={Characteristics of Some Multiply Recombination-Deficient Strains of Escherichia coli}, volume={100}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.100.1.231-239.1969}, DOI={10.1128/jb.100.1.231-239.1969}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Willetts, N. S. and Clark, A. J.}, year={1969}, month=oct, pages={231–239} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        24
      ]
    ],
    "date-time": "2025-08-24T01:54:52Z",
    "timestamp": 1756000492722
  },
  "reference-count": 15,
  "publisher": "American Society for Microbiology",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1969,
            10,
            1
          ]
        ],
        "date-time": "1969-10-01T00:00:00Z",
        "timestamp": -7948800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1969,
        10
      ]
    ]
  },
  "abstract": "<jats:p>\n            Strains of\n            <jats:italic>Escherichia coli</jats:italic>\n            have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified:\n            <jats:italic>recC22 recB21 recA</jats:italic>\n            <jats:sup>+</jats:sup>\n            ,\n            <jats:italic>recC22 recB21 recA13, recC22 recB</jats:italic>\n            <jats:sup>+</jats:sup>\n            <jats:italic>recA13</jats:italic>\n            , and\n            <jats:italic>recC</jats:italic>\n            <jats:sup>+</jats:sup>\n            <jats:italic>recB21 recA13</jats:italic>\n            . All multiple\n            <jats:italic>rec</jats:italic>\n            <jats:sup>\u2212</jats:sup>\n            strains carrying\n            <jats:italic>recA13</jats:italic>\n            were similar to AB2463, which carries\n            <jats:italic>recA13</jats:italic>\n            alone, in their UV sensitivities, recombination deficiencies, and inabilities to induce lambda phage in a lysogen. However, whereas AB2463 shows a high rate of ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown, the multiple\n            <jats:italic>rec</jats:italic>\n            <jats:sup>\u2212</jats:sup>\n            strains showed the low level characteristic of strains carrying\n            <jats:italic>recC22</jats:italic>\n            or\n            <jats:italic>recB21</jats:italic>\n            alone. The strain carrying both\n            <jats:italic>recC22</jats:italic>\n            and\n            <jats:italic>recB21</jats:italic>\n            was similar in all properties to the single mutants, suggesting that both gene products act in the same part of the recombination and UV repair pathways. It is concluded that in a Rec\n            <jats:sup>+</jats:sup>\n            strain, the\n            <jats:italic>recA</jats:italic>\n            <jats:sup>+</jats:sup>\n            product acts to inhibit DNA breakdown determined by the\n            <jats:italic>recC</jats:italic>\n            <jats:sup>+</jats:sup>\n            and\n            <jats:italic>recB</jats:italic>\n            <jats:sup>+</jats:sup>\n            products.\n          </jats:p>",
  "DOI": "10.1128/jb.100.1.231-239.1969",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        3
      ]
    ],
    "date-time": "2020-01-03T14:22:18Z",
    "timestamp": 1578061338000
  },
  "page": "231-239",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 225,
  "title": "Characteristics of Some Multiply Recombination-Deficient Strains of\n            <i>Escherichia coli</i>",
  "prefix": "10.1128",
  "volume": "100",
  "author": [
    {
      "given": "N. S.",
      "family": "Willetts",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, University of California, Berkeley, California 94720"
        }
      ]
    },
    {
      "given": "A. J.",
      "family": "Clark",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, University of California, Berkeley, California 94720"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1128/jb.79.3.321-330.1960",
      "article-title": "Genetic variation in the sex factor of Escherichia coli",
      "volume": "79",
      "author": "Adelberg E. A.",
      "year": "1960",
      "unstructured": "Adelberg , E. A. , and S. N. Burns . 1960 . Genetic variation in the sex factor of Escherichia coli . J. Bacteriol. 79 : 321 - 330 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "283",
      "DOI": "10.1128/jvi.1.2.283-293.1967",
      "article-title": "Behavior of bacteriophage in a recombination deficient strain of Escherichia coli",
      "volume": "1",
      "author": "Brooks K.",
      "year": "1967",
      "unstructured": "Brooks , K. , and A. J. Clark . 1967 . Behavior of bacteriophage in a recombination deficient strain of Escherichia coli . J. Virol. 1 : 283 - 293 .",
      "journal-title": "J. Virol."
    },
    {
      "key": "p_3",
      "first-page": "259",
      "article-title": "DNA breakdown in E. coli",
      "volume": "33",
      "author": "Buttin G.",
      "year": "1968",
      "unstructured": "Buttin , G. , and M. R. Wright . 1968 . DNA breakdown in E. coli . Cold Spring Harbor Symp. Quant. Biol. 33 : 259 - 269 .",
      "journal-title": "Cold Spring Harbor Symp. Quant. Biol."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "unstructured": "Clark A. J. 1967. The beginning of a genetic analysis of recombination proficiency. J. Cell. Physiol. 70(Suppl.)1: 165-180.",
      "DOI": "10.1002/jcp.1040700412"
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "442",
      "DOI": "10.1016/S0022-2836(66)80015-3",
      "article-title": "Abnormal metabolic response to ultraviolet light of a recombination-deficient mutant of Escherichia coli K12",
      "volume": "19",
      "author": "Clark A. J.",
      "year": "1966",
      "unstructured": "Clark , A. J. , M. Chamberlin , R. P. Boyce , and P. Howard-Flanders . 1966 . Abnormal metabolic response to ultraviolet light of a recombination-deficient mutant of Escherichia coli K12 . J. Mol. Biol. 19 : 442 - 454 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "451",
      "DOI": "10.1073/pnas.53.2.451",
      "article-title": "Isolation and characterization of recombination-deficient mutants of Escherichia coli K12",
      "volume": "53",
      "author": "Clark A. J.",
      "year": "1965",
      "unstructured": "Clark , A. J. , and A. D. Margulies . 1965 . Isolation and characterization of recombination-deficient mutants of Escherichia coli K12 . Proc. Nat. Acad. Sci. U.S.A. 53 : 451 - 459 .",
      "journal-title": "Proc. Nat. Acad. Sci. U.S.A."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1093/genetics/60.1.19",
      "article-title": "Recombination deficient mutants of Escherichia coli K12 that map between thyA and argA",
      "volume": "60",
      "author": "Emmerson P. T.",
      "year": "1968",
      "unstructured": "Emmerson , P. T. 1968 . Recombination deficient mutants of Escherichia coli K12 that map between thyA and argA . Genetics 60 : 19 - 30 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "1729",
      "DOI": "10.1128/jb.93.5.1729-1731.1967",
      "article-title": "Cotransduction with thy of a gene required for genetic recombination in E. coli K-12",
      "volume": "93",
      "author": "Emmerson P. T.",
      "year": "1967",
      "unstructured": "Emmerson , P. T. , and P. Howard-Flanders . 1967 . Cotransduction with thy of a gene required for genetic recombination in E. coli K-12 . J. Bacteriol. 93 : 1729 - 1731 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "117",
      "DOI": "10.1016/S0022-2836(67)80021-4",
      "article-title": "TransJuction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda",
      "volume": "23",
      "author": "Hertman I.",
      "year": "1967",
      "unstructured": "Hertman , I. , and S. E. Luria . 1967 . TransJuction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda . J. Mol. Biol. 23 : 117 - 133 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_10",
      "first-page": "156",
      "article-title": "DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes",
      "volume": "6",
      "author": "Howard-Flanders P.",
      "year": "1966",
      "unstructured": "Howard-Flanders , P. , and R. P. Boyce . 1966 . DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes . Radiat. Res. 6(Suppl. ): 156 - 184 .",
      "journal-title": "Radiat. Res."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "1137",
      "DOI": "10.1093/genetics/53.6.1137",
      "article-title": "Mutants of Escherichia coli K12 defective in DNA repair and in genetic recombination",
      "volume": "53",
      "author": "Howard-Flanders P.",
      "year": "1966",
      "unstructured": "Howard-Flanders , P. , and L. Theriot . 1966 . Mutants of Escherichia coli K12 defective in DNA repair and in genetic recombination . Genetics 53 : 1137 - 1150 .",
      "journal-title": "Genetics"
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "1134",
      "DOI": "10.1128/jb.97.3.1134-1141.1969",
      "article-title": "Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12",
      "volume": "97",
      "author": "Ila P.",
      "year": "1969",
      "unstructured": "Ila . Howard-Flanders, P. , L. Theriot , and J. B. Stedeford . 1969 . Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12 . J. Bacteriol. 97 : 1134 - 1141 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "160",
      "DOI": "10.1073/pnas.60.1.160",
      "article-title": "Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12",
      "volume": "60",
      "author": "Low B.",
      "year": "1968",
      "unstructured": "Low , B. 1968 . Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12 . Proc. Natl. Acad. Sci. U.S.A. 60 : 160 - 167 .",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "332",
      "DOI": "10.1128/br.31.4.332-353.1967",
      "article-title": "Revised linkage map of Escherichia coli",
      "volume": "31",
      "author": "Taylor A. L.",
      "year": "1967",
      "unstructured": "Taylor , A. L. , and C. D. Trotter . 1967 . Revised linkage map of Escherichia coli . Bacteriol. Rev. 31 : 332 - 353 .",
      "journal-title": "Bacteriol. Rev."
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "244",
      "DOI": "10.1128/jb.97.1.244-249.1969",
      "article-title": "Genetic location of certain mutations conferring recombination deficiency in Escherichia coli",
      "volume": "97",
      "author": "Willetts N. S.",
      "year": "1969",
      "unstructured": "Willetts , N. S. , A. J. Clark , and B. Low . 1969 . Genetic location of certain mutations conferring recombination deficiency in Escherichia coli . J. Bacteriol. 97 : 244 - 249 .",
      "journal-title": "J. Bacteriol."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.100.1.231-239.1969",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.100.1.231-239.1969",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T16:49:08Z",
    "timestamp": 1627577348000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.100.1.231-239.1969"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1969,
        10
      ]
    ]
  },
  "references-count": 15,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1969,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.100.1.231-239.1969"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.100.1.231-239.1969",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1969,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}