The Zinc-Responsive Regulator Zur Controls a Zinc Uptake System and Some Ribosomal Proteins inStreptomyces coelicolorA3(2)
10.1128/jb.01851-06
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTIn various bacteria, Zur, a zinc-specific regulator of the Fur family, regulates genes for zinc transport systems to maintain zinc homeostasis. It has also been suggested that Zur controls zinc mobilization by regulating some ribosomal proteins. The antibiotic-producing soil bacteriumStreptomyces coelicolorcontains four genes for Fur family regulators, and one (namedzur) is located downstream of theznuACBoperon encoding a putative zinc uptake transporter. We found that zinc specifically repressed the level ofznuAtranscripts and that this level was derepressed in a Δzurmutant. Purified Zur existing as homodimers bound to theznuApromoter region in the presence of zinc, confirming the role of Zur as a zinc-responsive repressor. We analyzed transcripts for paralogous forms of ribosomal proteins L31 (RpmE1 and RpmE2) and L33 (RpmG2 and RpmG3) for their dependence on Zur and found that RpmE2 and RpmG2 with no zinc-binding motif of conserved cysteines (C's) were negatively regulated by Zur. C-negative RpmG3 and C-positive RpmE1 were not regulated by Zur. Instead, they were regulated by the sigma factor σRas predicted from their promoter sequences. TherpmE1andrpmG3genes were partially induced by EDTA in a manner dependent on σR, suggesting that zinc depletion may stimulate the σRregulatory system. This finding reflects a link between thiol-oxidizing stress and zinc depletion. We determined the Zur-binding sites withinznuAandrpmG2promoter regions by footprinting analyses and identified a consensus inverted repeat sequence (TGaaAatgatTttCA, where uppercase letters represent the nucleotides common to all sites analyzed). This sequence closely matches that for mycobacterial Zur and allows the prediction of more genes in the Zur regulon.

Bibliography

Shin, J.-H., Oh, S.-Y., Kim, S.-J., & Roe, J.-H. (2007). The Zinc-Responsive Regulator Zur Controls a Zinc Uptake System and Some Ribosomal Proteins inStreptomyces coelicolorA3(2). Journal of Bacteriology, 189(11), 4070–4077.

Authors 4
  1. Jung-Ho Shin (first)
  2. So-Young Oh (additional)
  3. Soon-Jong Kim (additional)
  4. Jung-Hye Roe (additional)
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Dates
Type When
Created 18 years, 4 months ago (April 6, 2007, 8:15 p.m.)
Deposited 1 year, 6 months ago (Feb. 13, 2024, 1:47 a.m.)
Indexed 1 month ago (July 26, 2025, 5:11 a.m.)
Issued 18 years, 2 months ago (June 1, 2007)
Published 18 years, 2 months ago (June 1, 2007)
Published Print 18 years, 2 months ago (June 1, 2007)
Funders 0

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@article{Shin_2007, title={The Zinc-Responsive Regulator Zur Controls a Zinc Uptake System and Some Ribosomal Proteins inStreptomyces coelicolorA3(2)}, volume={189}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.01851-06}, DOI={10.1128/jb.01851-06}, number={11}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Shin, Jung-Ho and Oh, So-Young and Kim, Soon-Jong and Roe, Jung-Hye}, year={2007}, month=jun, pages={4070–4077} }