Characterization and Evolution of Cell Division and Cell Wall Synthesis Genes in the Bacterial Phyla Verrucomicrobia , Lentisphaerae , Chlamydiae , and Planctomycetes and Phylogenetic Comparison with rRNA Genes
10.1128/jb.01797-07
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT In the past, studies on the relationships of the bacterial phyla Planctomycetes , Chlamydiae , Lentisphaerae , and Verrucomicrobia using different phylogenetic markers have been controversial. Investigations based on 16S rRNA sequence analyses suggested a relationship of the four phyla, showing the branching order Planctomycetes , Chlamydiae , Verrucomicrobia / Lentisphaerae . Phylogenetic analyses of 23S rRNA genes in this study also support a monophyletic grouping and their branching order—this grouping is significant for understanding cell division, since the major bacterial cell division protein FtsZ is absent from members of two of the phyla Chlamydiae and Planctomycetes . In Verrucomicrobia , knowledge about cell division is mainly restricted to the recent report of ftsZ in the closely related genera Prosthecobacter and Verrucomicrobium . In this study, genes of the conserved division and cell wall ( dcw ) cluster ( ddl , ftsQ , ftsA , and ftsZ ) were characterized in all verrucomicrobial subdivisions (1 to 4) with cultivable representatives (1 to 4). Sequence analyses and transcriptional analyses in Verrucomicrobia and genome data analyses in Lentisphaerae suggested that cell division is based on FtsZ in all verrucomicrobial subdivisions and possibly also in the sister phylum Lentisphaerae . Comprehensive sequence analyses of available genome data for representatives of Verrucomicrobia , Lentisphaerae , Chlamydiae , and Planctomycetes strongly indicate that their last common ancestor possessed a conserved, ancestral type of dcw gene cluster and an FtsZ-based cell division mechanism. This implies that Planctomycetes and Chlamydiae may have shifted independently to a non-FtsZ-based cell division mechanism after their separate branchings from their last common ancestor with Verrucomicrobia .

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Authors 7
  1. Martin Pilhofer (first)
  2. Kristina Rappl (additional)
  3. Christina Eckl (additional)
  4. Andreas Peter Bauer (additional)
  5. Wolfgang Ludwig (additional)
  6. Karl-Heinz Schleifer (additional)
  7. Giulio Petroni (additional)
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Dates
Type When
Created 17 years, 5 months ago (Feb. 29, 2008, 9:36 p.m.)
Deposited 4 years ago (July 29, 2021, 1:45 p.m.)
Indexed 27 minutes ago (Aug. 29, 2025, 5:28 a.m.)
Issued 17 years, 3 months ago (May 1, 2008)
Published 17 years, 3 months ago (May 1, 2008)
Published Print 17 years, 3 months ago (May 1, 2008)
Funders 0

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@article{Pilhofer_2008, title={Characterization and Evolution of Cell Division and Cell Wall Synthesis Genes in the Bacterial Phyla Verrucomicrobia , Lentisphaerae , Chlamydiae , and Planctomycetes and Phylogenetic Comparison with rRNA Genes}, volume={190}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.01797-07}, DOI={10.1128/jb.01797-07}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Pilhofer, Martin and Rappl, Kristina and Eckl, Christina and Bauer, Andreas Peter and Ludwig, Wolfgang and Schleifer, Karl-Heinz and Petroni, Giulio}, year={2008}, month=may, pages={3192–3202} }