DOI: 10.1128/jb.01562-09. Role of FlgT in Anchoring the Flagellum of <i>Vibrio cholerae</i>

Role of FlgT in Anchoring the Flagellum of Vibrio cholerae

10.1128/jb.01562-09

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Flagellar motility has long been regarded as an important virulence factor. In Vibrio cholerae , the single polar flagellum is essential for motility as well as for proper attachment and colonization. In this study, we demonstrate that the novel flagellar protein FlgT is involved in anchoring the flagellum to the V. cholerae cell. A screen for novel colonization factors by use of Tn phoA mutagenesis identified flgT . An in-frame deletion of flgT established that FlgT is required for attachment, colonization, and motility. Transmission electron microscopy revealed that while the flgT mutant is capable of assembling a phenotypically normal flagellum, the flgT population is mostly aflagellate compared to the wild-type population. Further analyses indicated that the flagellum of the flgT mutant is released into the culture supernatant from the cell upon completion of assembly. Additionally, hook basal body complexes appear to be released along with the filament. These results indicate that FlgT functions to stabilize the flagellar apparatus at the pole of the cell.

Bibliography

Martinez, R. M., Jude, B. A., Kirn, T. J., Skorupski, K., & Taylor, R. K. (2010). Role of FlgT in Anchoring the Flagellum of Vibrio cholerae. Journal of Bacteriology, 192(8), 2085â2092.

Authors 5

Raquel M. Martinez (first)
Brooke A. Jude (additional)
Thomas J. Kirn (additional)
Karen Skorupski (additional)
Ronald K. Taylor (additional)

References 46 Referenced 31

Dates

Type	When
Created	15 years, 6 months ago (Feb. 12, 2010, 9:09 p.m.)
Deposited	4 years ago (July 29, 2021, 1:16 p.m.)
Indexed	1 year ago (Aug. 20, 2024, 4:34 a.m.)
Issued	15 years, 4 months ago (April 15, 2010)
Published	15 years, 4 months ago (April 15, 2010)
Published Print	15 years, 4 months ago (April 15, 2010)

Funders 0

None

BibTeX

@article{Martinez_2010, title={Role of FlgT in Anchoring the Flagellum of Vibrio cholerae}, volume={192}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.01562-09}, DOI={10.1128/jb.01562-09}, number={8}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Martinez, Raquel M. and Jude, Brooke A. and Kirn, Thomas J. and Skorupski, Karen and Taylor, Ronald K.}, year={2010}, month=apr, pages={2085–2092} }

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