DOI: 10.1128/jb.01513-08. Chromatin Organization and Radio Resistance in the Bacterium <i>Gemmata obscuriglobus</i>

Chromatin Organization and Radio Resistance in the Bacterium Gemmata obscuriglobus
10.1128/jb.01513-08
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract
ABSTRACT The organization of chromatin has a major impact on cellular activities, such as gene expression. For bacteria, it was suggested that the spatial organization of the genetic material correlates with transcriptional levels, implying a specific architecture of the chromosome within the cytoplasm. Accordingly, recent technological advances have emphasized the organization of the genetic material within nucleoid structures. Gemmata obscuriglobus , a member of the phylum Planctomycetes , exhibits a distinctive nucleoid structure in which chromatin is encapsulated within a discrete membrane-bound compartment. Here, we show that this soil and freshwater bacterium tolerates high doses of UV and ionizing radiation. Cryoelectron tomography of frozen hydrated sections and electron microscopy of freeze-substituted cells have indicated a more highly ordered condensed-chromatin organization in actively dividing and stationary-phase G. obscuriglobus cells. These three-dimensional analyses revealed a complex network of double membranes that engulf the condensed DNA. Bioinformatics analysis has revealed the existence of a putative component involved in nonhomologous DNA end joining that presumably plays a role in maintaining chromatin integrity within the bacterium. Thus, our observations further support the notion that packed chromatin organization enhances radiation tolerance.
Bibliography
Lieber, A., Leis, A., Kushmaro, A., Minsky, A., & Medalia, O. (2009). Chromatin Organization and Radio Resistance in the Bacterium Gemmata obscuriglobus. Journal of Bacteriology, 191(5), 1439â1445.
Authors 5
Arnon Lieber (first)
Andrew Leis (additional)
Ariel Kushmaro (additional)
Abraham Minsky (additional)
Ohad Medalia (additional)
References 59 Referenced 45
Dates
Type	When
Created	16 years, 8 months ago (Dec. 12, 2008, 9:48 p.m.)
Deposited	4 years ago (July 29, 2021, 1:10 p.m.)
Indexed	1 year, 2 months ago (June 3, 2024, 3:17 a.m.)
Issued	16 years, 5 months ago (March 1, 2009)
Published	16 years, 5 months ago (March 1, 2009)
Published Print	16 years, 5 months ago (March 1, 2009)
Funders 0
None
BibTeX
@article{Lieber_2009, title={Chromatin Organization and Radio Resistance in the Bacterium Gemmata obscuriglobus}, volume={191}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.01513-08}, DOI={10.1128/jb.01513-08}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Lieber, Arnon and Leis, Andrew and Kushmaro, Ariel and Minsky, Abraham and Medalia, Ohad}, year={2009}, month=mar, pages={1439–1445} }
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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The organization of chromatin has a major impact on cellular activities, such as gene expression. For bacteria, it was suggested that the spatial organization of the genetic material correlates with transcriptional levels, implying a specific architecture of the chromosome within the cytoplasm. Accordingly, recent technological advances have emphasized the organization of the genetic material within nucleoid structures.\n            <jats:italic>Gemmata obscuriglobus</jats:italic>\n            , a member of the phylum\n            <jats:italic>Planctomycetes</jats:italic>\n            , exhibits a distinctive nucleoid structure in which chromatin is encapsulated within a discrete membrane-bound compartment. Here, we show that this soil and freshwater bacterium tolerates high doses of UV and ionizing radiation. Cryoelectron tomography of frozen hydrated sections and electron microscopy of freeze-substituted cells have indicated a more highly ordered condensed-chromatin organization in actively dividing and stationary-phase\n            <jats:italic>G. obscuriglobus</jats:italic>\n            cells. These three-dimensional analyses revealed a complex network of double membranes that engulf the condensed DNA. Bioinformatics analysis has revealed the existence of a putative component involved in nonhomologous DNA end joining that presumably plays a role in maintaining chromatin integrity within the bacterium. Thus, our observations further support the notion that packed chromatin organization enhances radiation tolerance.\n          </jats:p>",
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