DOI: 10.1128/jb.00946-09. Cag3 Is a Novel Essential Component of the<i>Helicobacter pylori</i>Cag Type IV Secretion System Outer Membrane Subcomplex

Cag3 Is a Novel Essential Component of theHelicobacter pyloriCag Type IV Secretion System Outer Membrane Subcomplex

10.1128/jb.00946-09

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTHelicobacter pyloristrains harboring thecagpathogenicity island (PAI) have been associated with more severe gastric disease in infected humans. ThecagPAI encodes a type IV secretion (T4S) system required for CagA translocation into host cells as well as induction of proinflammatory cytokines, such as interleukin-8 (IL-8).cagPAI genes sharing sequence similarity with T4S components from other bacteria are essential for Cag T4S function. OthercagPAI-encoded genes are also essential for Cag T4S, but lack of sequence-based or structural similarity with genes in existing databases has precluded a functional assignment for the encoded proteins. We have studied the role of one such protein, Cag3 (HP0522), in Cag T4S and determined Cag3 subcellular localization and protein interactions. Cag3 is membrane associated and copurifies with predicted inner and outer membrane Cag T4S components that are essential for Cag T4S as well as putative accessory factors. Coimmunoprecipitation and cross-linking experiments revealed specific interactions with HpVirB7 and CagM, suggesting Cag3 is a new component of the Cag T4S outer membrane subcomplex. Finally, lack of Cag3 lowers HpVirB7 steady-state levels, further indicating Cag3 makes a subcomplex with this protein.

Bibliography

Pinto-Santini, D. M., & Salama, N. R. (2009). Cag3 Is a Novel Essential Component of theHelicobacter pyloriCag Type IV Secretion System Outer Membrane Subcomplex. Journal of Bacteriology, 191(23), 7343â7352.

Authors 2

Delia M. Pinto-Santini (first)
Nina R. Salama (additional)

References 38 Referenced 42

Dates

Type	When
Created	15 years, 10 months ago (Oct. 3, 2009, 9:42 a.m.)
Deposited	1 year, 5 months ago (March 16, 2024, 6:20 p.m.)
Indexed	4 months, 2 weeks ago (April 11, 2025, 5:50 a.m.)
Issued	15 years, 9 months ago (Dec. 1, 2009)
Published	15 years, 9 months ago (Dec. 1, 2009)
Published Print	15 years, 9 months ago (Dec. 1, 2009)

Funders 0

None

BibTeX

@article{Pinto_Santini_2009, title={Cag3 Is a Novel Essential Component of theHelicobacter pyloriCag Type IV Secretion System Outer Membrane Subcomplex}, volume={191}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00946-09}, DOI={10.1128/jb.00946-09}, number={23}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Pinto-Santini, Delia M. and Salama, Nina R.}, year={2009}, month=dec, pages={7343–7352} }

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