DOI: 10.1128/jb.00908-08. Bacterial Flagella Are Firmly Anchored

10.1128/jb.00908-08

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT There are mutants of Salmonella enterica (with mutations in fliF and fliL ) that shed flagella when they are swimming in a viscous medium or on the surface of soft agar. Filaments with hooks and the distal rod segment FlgG are recovered. We tried to extract flagellar filaments from such cells by pulling on them with an optical trap but failed, even when we used forces large enough to straighten the filaments. Thus, flagella are firmly anchored.

Bibliography

Darnton, N. C., & Berg, H. C. (2008). Bacterial Flagella Are Firmly Anchored. Journal of Bacteriology, 190(24), 8223â8224.

Authors 2

Nicholas C. Darnton (first)
Howard C. Berg (additional)

References 11 Referenced 10

Attmannspacher, U., B. E. Scharf, and R. M. Harshey. 2008. FliL is essential for swarming: motor rotation in absence of FliL fractures the flagellar rod in swarmer cells of Salmonella enterica. Mol. Microbiol. 68 : 328-341. (10.1111/j.1365-2958.2008.06170.x) / Mol. Microbiol. (2008)
Random walks in biology 1993
Berg-Sørensen, K., and H. Flyvbjerg. 2004. Power spectrum analysis for optical tweezers. Rev. Sci. Instrum. 75 : 594-612. (10.1063/1.1645654) / Rev. Sci. Instrum. (2004)
Darnton, N. C., and H. C. Berg. 2007. Force-extension measurements on bacterial flagella: triggering polymorphic transformations. Biophys. J. 92 : 2230-2236. (10.1529/biophysj.106.094037) / Biophys. J. (2007)
10.1128/JB.01501-06
Fahrner, K. A., S. M. Block, S. Krishnaswamy, J. S. Parkinson, and H. C. Berg. 1994. A mutant hook-associated protein (HAP3) facilitates torsionally-induced transformations of the flagellar filament of Escherichia coli. J. Mol. Biol. 238 : 173-186. (10.1006/jmbi.1994.1279) / J. Mol. Biol. (1994)
Kamiya, R., and S. Asakura. 1976. Flagellar transformations of Salmonella flagella in vitro. J. Mol. Biol. 106 : 167-186. (10.1016/0022-2836(76)90306-5) / J. Mol. Biol. (1976)
Macnab, R. M., and M. K. Ornston. 1977. Normal-to-curly flagellar transitions and their role in bacterial tumbling: stabilization of an alternative quaternary structure by mechanical force. J. Mol. Biol. 112 : 1-30. (10.1016/S0022-2836(77)80153-8) / J. Mol. Biol. (1977)
10.1128/jb.171.4.2075-2082.1989
10.1128/JB.182.10.2793-2801.2000
Yamaguchi, S., H. Fujita, K. Sugata, T. Taira, and T. Iino. 1984. Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella. J. Gen. Microbiol. 130 : 255-265. / J. Gen. Microbiol. (1984)

Dates

Type	When
Created	16 years, 10 months ago (Oct. 10, 2008, 8:45 p.m.)
Deposited	4 years ago (July 29, 2021, 1:42 p.m.)
Indexed	1 year, 6 months ago (Feb. 1, 2024, 1:44 p.m.)
Issued	16 years, 8 months ago (Dec. 15, 2008)
Published	16 years, 8 months ago (Dec. 15, 2008)
Published Print	16 years, 8 months ago (Dec. 15, 2008)

Funders 0

None

BibTeX

@article{Darnton_2008, title={Bacterial Flagella Are Firmly Anchored}, volume={190}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00908-08}, DOI={10.1128/jb.00908-08}, number={24}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Darnton, Nicholas C. and Berg, Howard C.}, year={2008}, month=dec, pages={8223–8224} }

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