DOI: 10.1128/jb.00856-08. Role of Two NtcA-Binding Sites in the Complex <i>ntcA</i> Gene Promoter of the Heterocyst-Forming Cyanobacterium <i>Anabaena</i> sp. Strain PCC 7120

Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120

10.1128/jb.00856-08

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N 2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression.

Bibliography

Olmedo-Verd, E., Valladares, A., Flores, E., Herrero, A., & Muro-Pastor, A. M. (2008). Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. Journal of Bacteriology, 190(22), 7584â7590.

Authors 5

Elvira Olmedo-Verd (first)
Ana Valladares (additional)
Enrique Flores (additional)
Antonia Herrero (additional)
Alicia M. Muro-Pastor (additional)

References 28 Referenced 14

Current protocols in molecular biology. 2007
Black, T. A., Y. Cai, and C. P. Wolk. 1993. Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol. 9 : 77-84. (10.1111/j.1365-2958.1993.tb01670.x) / Mol. Microbiol. (1993)
Buikema, W. J., and R. Haselkorn. 1991. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Dev. 5 : 321-330. (10.1101/gad.5.2.321) / Genes Dev. (1991)
10.1128/jb.172.6.3138-3145.1990
Frías, J. E., E. Flores, and A. Herrero. 1994. Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC 7120. Mol. Microbiol. 14 : 823-832. (10.1111/j.1365-2958.1994.tb01318.x) / Mol. Microbiol. (1994)
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10.1128/JB.183.2.411-425.2001
Herrero, A., A. M. Muro-Pastor, A. Valladares, and E. Flores. 2004. Cellular differentiation and the NtcA transcription factor in filamentous cyanobacteria. FEMS Microbiol. Rev. 28 : 469-487. (10.1016/j.femsre.2004.04.003) / FEMS Microbiol. Rev. (2004)
Huang, X., Y. Dong, and J. Zhao. 2004. HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc. Natl. Acad. Sci. USA 101 : 4848-4853. (10.1073/pnas.0400429101) / Proc. Natl. Acad. Sci. USA (2004)
Laurent, S., H. Chen, S. Bédu, F. Ziarelli, L. Peng, and C.-C. Zhang. 2005. Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120. Proc. Natl. Acad. Sci. USA 102 : 9907-9912. (10.1073/pnas.0502337102) / Proc. Natl. Acad. Sci. USA (2005)
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10.1128/JB.181.21.6664-6669.1999
Muro-Pastor, A. M., A. Valladares, E. Flores, and A. Herrero. 2002. Mutual dependence of the expression of the cell differentiation regulatory protein HetR and the global nitrogen regulator NtcA during heterocyst development. Mol. Microbiol. 44 : 1377-1385. (10.1046/j.1365-2958.2002.02970.x) / Mol. Microbiol. (2002)
Muro-Pastor, M. I., J. C. Reyes, and F. J. Florencio. 2001. Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276 : 38320-38328. (10.1074/jbc.M105297200) / J. Biol. Chem. (2001)
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10.1128/jb.176.5.1214-1223.1994
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Tanigawa, R., M. Shirokane, S.-I. Maeda, T. Omata, K. Tanaka, and H. Takahashi. 2002. Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. USA 99 : 4251-4255. (10.1073/pnas.072587199) / Proc. Natl. Acad. Sci. USA (2002)
10.1128/JB.00787-08
Vázquez-Bermúdez, M. F., E. Flores, and A. Herrero. 2002. Analysis of binding sites for the nitrogen-control transcription factor NtcA in the promoters of Synechococcus nitrogen-regulated genes. Biochim. Biophys. Acta 1578 : 95-98. (10.1016/S0167-4781(02)00506-7) / Biochim. Biophys. Acta (2002)
Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2002. 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett. 512 : 71-74. (10.1016/S0014-5793(02)02219-6) / FEBS Lett. (2002)
Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2003. Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942. FEMS Microbiol. Lett. 221 : 155-159. (10.1016/S0378-1097(03)00208-8) / FEMS Microbiol. Lett. (2003)
10.1128/jb.176.15.4473-4482.1994
Xu, X., J. Elhai, and C. P. Wolk. 2008. Transcriptional and developmental responses by Anabaena to deprivation of fixed nitrogen, p. 383-422. In A. Herrero and E. Flores (ed.), The cyanobacteria. Molecular biology, genomics and evolution. Caister Academic Press, Norfolk, United Kingdom. / The cyanobacteria. (2008)
Zhou, R., X. Wei, N. Jiang, H. Li, Y. Dong, K.-L. Hsi, and J. Zhao. 1998. Evidence that HetR protein is an unusual serine-type protease. Proc. Natl. Acad. Sci. USA 95 : 4959-4963. (10.1073/pnas.95.9.4959) / Proc. Natl. Acad. Sci. USA (1998)

Dates

Type	When
Created	16 years, 11 months ago (Sept. 19, 2008, 9:07 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:43 p.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 11:13 a.m.)
Issued	16 years, 9 months ago (Nov. 15, 2008)
Published	16 years, 9 months ago (Nov. 15, 2008)
Published Print	16 years, 9 months ago (Nov. 15, 2008)

Funders 0

None

BibTeX

@article{Olmedo_Verd_2008, title={Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120}, volume={190}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00856-08}, DOI={10.1128/jb.00856-08}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Olmedo-Verd, Elvira and Valladares, Ana and Flores, Enrique and Herrero, Antonia and Muro-Pastor, Alicia M.}, year={2008}, month=nov, pages={7584–7590} }

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